We narrowed to 13,216 results for: CRISPR-Cas9

1. pLL3.7-hSt3Cas9 (pXK-1721)

   Plasmid

   #208828

   Purpose

   CRISPR/hSt3Cas9 expression vector for Animal gene editing, harboring the humanized St3Cas9 gene and the corresponding optimized SP1.gRNA. Key Construct: mU6- SP1.gRNA-CMV-hSt3Cas9.

   Depositor

   Kun Xu

   Article

   Xu et al Cell Mol Life Sci. 2015 Jan;72(2):383-99

   Type

   Empty backbone

   Use

   CRISPR; Stcas9

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-dHeFSpCas9-VP64-6xHis

   Plasmid

   #92119

   Purpose

   Expression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive HeFSpCas9-NLS-3xFLAG-VP64
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, NLS, and VP64

   Expression

   Bacterial

   Mutation

   D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

   Promoter

   T7

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. PET-HypaSpCas9-NLS-6xHis

   Plasmid

   #207384

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   HypaSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N692A, M694A, Q695A, H698A

   Promoter

   T7

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. PET-HeFSpCas9-NLS-6xHis

   Plasmid

   #207391

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   HeFSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

   Promoter

   T7

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PET-evoSpCas9-NLS-6xHis

   Plasmid

   #207389

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   evoSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M495V, Y515N, K526E, R661Q

   Promoter

   T7

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. nSaCas9 P742 miniABEMax V82G

   Plasmid

   #135385

   Purpose

   Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (P742 )

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSaCas9 P742 miniABEMax V82G
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   (V82G)

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. nSaCas9 Q731 miniABEMax V82G

   Plasmid

   #135386

   Purpose

   Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (Q731 )

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSaCas9 Q731 miniABEMax V82G
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   (V82G)

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. nSaCas9 Q737 miniABEMax V82G

   Plasmid

   #135387

   Purpose

   Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (Q737 )

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSaCas9 Q737 miniABEMax V82G
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   (V82G)

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. nSaCas9 S740 miniABEMax V82G

   Plasmid

   #135388

   Purpose

   Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (S740 )

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSaCas9 S740 miniABEMax V82G
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   (V82G)

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. nSaCas9 T128 miniABEMax V82G

    Plasmid

    #135389

    Purpose

    Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (T128 )

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSaCas9 T128 miniABEMax V82G
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. nSaCas9 D127 miniABEMax V82G

    Plasmid

    #135366

    Purpose

    Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (D127 )

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSaCas9 D127 miniABEMax V82G
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. nSaCas9 D126 miniABEMax V82G

    Plasmid

    #135367

    Purpose

    Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (D126 )

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSaCas9 D126 miniABEMax V82G
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. nSaCas9 E739 miniABEMax V82G

    Plasmid

    #135368

    Purpose

    Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E739 )

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSaCas9 E739 miniABEMax V82G
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. nSaCas9 E743 miniABEMax V82G

    Plasmid

    #135369

    Purpose

    Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E743 )

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSaCas9 E743 miniABEMax V82G
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. nSaCas9 E745 miniABEMax V82G

    Plasmid

    #135370

    Purpose

    Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E745 )

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSaCas9 E745 miniABEMax V82G
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. nSaCas9 F733 miniABEMax V82G

    Plasmid

    #135371

    Purpose

    Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (F733 )

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSaCas9 F733 miniABEMax V82G
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. nSaCas9 N730 miniABEMax V82G

    Plasmid

    #135372

    Purpose

    Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (N730 )

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSaCas9 N730 miniABEMax V82G
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. nSaCas9 E125 miniABEMax V82G

    Plasmid

    #135373

    Purpose

    Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E125 )

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSaCas9 E125 miniABEMax V82G
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. nSaCas9 E131 miniABEMax V82G

    Plasmid

    #135374

    Purpose

    Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E131 )

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSaCas9 E131 miniABEMax V82G
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. nSaCas9 L132 miniABEMax V82G

    Plasmid

    #135375

    Purpose

    Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (L132 )

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSaCas9 L132 miniABEMax V82G
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge