We narrowed to 5,900 results for: pcas

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pCA-G-intron(Neo)-T(FLLFLNL)

Plasmid

#40020

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tdT-3Myc
beta-globin intron
Neo

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta globin promoter and CMV enhance…

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pMS7: pCascade(AvCAST)_entry

Plasmid

#168140

Purpose

Inducible expression of AvCAST Cascade proteins. Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-AaCas12b(D977A)-2AeGFP

Plasmid

#121962

Purpose

Mammalian expression, Transcription regulation, deactivated AaCas12b

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

Catalytically deactivated AaCas12b

Tags

3xHA tag

Expression

Mammalian

Mutation

D977A

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBT374_pattB-pCA-GFP-pA-attBSwa

Plasmid

#52553

Purpose

for PhiC31 integrase-mediated cassette exchange

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Expression

Mammalian

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-hHIF1 alpha-IGd2

Plasmid

#22535

Purpose

To be used for the RNAi screening purposes (not for HDAC4 expression); see paper

Depositor

Connie Cepko

Article

Chen et al Science. 2009 Jan 9. 323(5911):256-9.

Insert

hypoxia inducible factor 1 alpha (HIF1A Human)

Expression

Mammalian

Available Since

Nov. 9, 2009

Availability

Academic Institutions and Nonprofits only
pCAG-MCP-VP64-2AmScarlet

Plasmid

#121963

Purpose

Mammalian expression, Transcription regulation module

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

VP64 module fused with MCP, recruited by MS2 hairpin

Expression

Mammalian

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-Exon4-PAFAH1B1

Plasmid

#92433

Purpose

expresses Cas9 and gRNA targeting PAFAH1B1

Depositor

Ralf Brandes, Matthias Leisegang

Article

Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

Insert

PAFAH1B1 (PAFAH1B1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS/Daxx myc S502A

Plasmid

#19281

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

Daxx myc (Daxx Mouse)

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pCAG-ChroME(F243Y)-mRuby2-ST

Plasmid

#109127

Purpose

Modified cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

ChroME(F243Y)-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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161+80pCalb2/mutCREB(-2)-like

Plasmid

#66751

Purpose

luciferase reporter for Calb2 promoter (-161to +80, mutant)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp +80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

GCGC (-2) to TAGA

Promoter

Calb2

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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-161+80pCalb2/mutNRF-1(-41)-like

Plasmid

#66744

Purpose

luciferase reporter for Calb2 promoter (-161to +80, mutant)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp+80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CGC (-41) to ATT

Promoter

Calb2

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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-161+80pCalb2/dmutNRF-1(-41;-35)-like

Plasmid

#66741

Purpose

luciferase reporter for Calb2 promoter (-161to +80, mutant)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp+80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CGCAGGCGC (-41;-35) to ATTAGGATT

Promoter

Calb2

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-Chronos(M140T)-mRuby2-ST

Plasmid

#109129

Purpose

Modified cation channelrhodopsin Chronos fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

Chronos(M140T)-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-NS1-HilA

Plasmid

#153327

Purpose

Triggers secretion of SptP120 fused to the NS1 monobody from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-NS1 Monobody

Use

Salmonella expression

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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Osa-pCAMBIA-1301-STTM408-3p

Plasmid

#84217

Purpose

Disturb the expression of miR408-3p in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR408-3p

Expression

Bacterial, Plant, and Yeast…

Promoter

2x35S

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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PX543 SpCas9(del175-307)

Plasmid

#58888

Purpose

Truncated form of SpCas9 without REC2 domain

Depositor

Feng Zhang

Article

Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Mutation

del amino acids 175-307

Available Since

Sept. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-eSpCas9n(1.1)-2A-EGFP-L2

Plasmid

#141052

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

eSpCas9(1.1)-2A-EGFP

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pCA-G-intron(Neo)-T(LNL)

Plasmid

#36907

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tdTomato-3Myc
beta-globin intron
Neo

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only

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Osa-pCAMBIA-1301-STTM396

Plasmid

#84206

Purpose

Disturb the expression of miR396 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR396

Expression

Bacterial, Plant, and Yeast…

Promoter

2x35S

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS-rat PKA-RIIbeta

Plasmid

#45529

Depositor

Haining Zhong

Article

Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

Insert

PKA regulatory subunit RII beta

Use

With chicken beta actin promoter and wpre

Expression

Mammalian

Available Since

July 18, 2013

Availability

Academic Institutions and Nonprofits only

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