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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 60,524 results for: Gene

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Showing: 2761 - 2790 of 60524 results

  4. pRelA'

    Plasmid
    #175595
    Purpose
    Tet inducible, KAN Resistant, non labelled synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.
    Depositor
    Greg Bokinsky
    Article
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    Catalytic domain of E.coli relA gene.
    Expression
    Bacterial
    Mutation
    Only the catalytic domain of the enzyme is used (…
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    175595-plasmid-map-sequence-id-348284

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  12. pLR16-Phes*

    Plasmid
    #98783
    Purpose
    Suicide vector for allelic exchange in Listeria utilizing an engineered counterselection marker for Listeria monocytogenes based on a mutated phenylalanyl-tRNA synthetase gene (pheS*).
    Depositor
    Anat Herskovits
    Article
    Argov et al Appl Environ Microbiol. 2017 Mar 2;83(6)
    Insert
    pheS
    Expression
    Bacterial
    Mutation
    A309G
    Available Since
    Jan. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    98783-plasmid-map-sequence-id-189220

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  14. pACT2-CAS9c

    Plasmid
    #51055
    Purpose
    Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.
    Depositor
    Yunde Zhao
    Article
    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1
    Insert
    Cas9c
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Bacterial and Yeast
    Promoter
    yeast ADH1 promoter
    Available Since
    Feb. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51055-plasmid-map-sequence-id-77327

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  15. pHMWGFPR

    Plasmid
    #64396
    Purpose
    High molecular weight glutenin gene promoter driving GFP expression
    Depositor
    Claudia Vickers
    Article
    Vickers et al Plant Mol Biol. 2006 Sep;62(1-2):195-214
    Insert
    High molecular weight glutenin gene promoter
    Tags
    GFP
    Expression
    Plant
    Promoter
    High molecular weight glutenin gene promoter
    Available Since
    June 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. pDONR223 SARS-CoV-2 N

    Plasmid
    #149330
    Purpose
    Gateway-compatible Entry vector, with insert of the nucleocapsid (N) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    N (N SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway Cloning
    Mutation
    Many synonymous changes due to codon optimization
    Available Since
    May 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149330-plasmid-map-sequence-id-296491

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  18. pAd-M3cherry

    Plasmid
    #61041
    Purpose
    This plasmid mediates polycistronic expression of 4 genes: Ngn3, Pdx1, Mafa, and mCherry. It can be used to convert mouse pancreatic acinar cells to induced beta-like cells in vivo.
    Depositor
    Qiao Zhou
    Article
    Li et al Nat Biotechnol. 2014 Dec;32(12):1223-30.
    Inserts
    Ngn3 (Neurog3 Mouse)
    pdx1 (Pdx1 Mouse)
    Mafa (Mafa Mouse)
    monomeric cherry
    Use
    Adenoviral
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    61041-plasmid-map-sequence-id-456476

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  19. pET28-Dnmt3a3L-sc27

    Plasmid
    #71827
    Purpose
    For bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.
    Depositor
    Albert Jeltsch
    Article
    Siddique et al J Mol Biol. 2013 Feb 8;425(3):479-91. do
    Insert
    Dnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)
    Tags
    6xHis tag
    Expression
    Bacterial
    Available Since
    Jan. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    71827-plasmid-map-sequence-id-254300

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  23. CRISPi Kit

    Plasmid Kit
    #1000000136
    Purpose
    Plasmids for performing CRISPR/Cas9 mediated gene and genome editing in Pichia pastoris.
    Depositor
    Brigitte Gasser
    Application
    Genome Editing
    Vector Type
    Yeast Expression
    Editing Type
    CRISPR
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only

  24. p-mCherry-C1-Phobos

    Plasmid
    #98166
    Purpose
    blue-shifted artificial anion conducting channelrhodopsin (aACR). Activation max 466 nm; off-kinetics 10 ms. Codon optimized for mammalian expression.
    Depositor
    Peter Hegemann
    Article
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct Phobos gene
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, T159G, G16…
    Promoter
    CMV (+enhancer)
    Available Since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98166-plasmid-map-sequence-id-182715

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  25. pUC19-E6MBW6

    Plasmid
    #103102
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    E6MBW6(Cas9 coding gene from Staphylococcus lugdunensis M23590)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    April 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    103102-plasmid-map-sequence-id-209054

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  26. pSMPUW_6.17_Ex_Ef1a-v5-EGFP-SV40-Zeo

    Plasmid
    #128453
    Purpose
    lentiviral expression vector with Ef1a promoter, N-terminal V5 tag, EGFP gene, SV40 promoter, and ZeoResistance gene
    Depositor
    Lindy Barrett
    Insert
    Ef1a promoter, N-terminal V5 tag, EGFP gene, SV40 promoter, and Zeocin Resistance gene
    Use
    Lentiviral
    Available Since
    Oct. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    128453-plasmid-map-sequence-id-261484

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Showing: 2761 - 2790 of 60524 results