We narrowed to 6,986 results for: Cad

1. pLentiGuide_mCherry_sgEPAS1#2

   Plasmid

   #183123

   Purpose

   pLentiGuide modified to express mCherry and an sgRNA targeting EPAS1

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   sgEPAS1-2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-ChrimsonR-LSSmOrange

   Plasmid

   #149691

   Purpose

   For packaging and expression of ChrimsonR-LSSmOrange in neurons via AAV

   Depositor

   Robert Malenka

   Article

   Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

   Insert

   ChrimsonR
   

   Use

   AAV

   Tags

   LSSmOrange

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEN_hU6miR-Luc-A

   Plasmid

   #25756

   Purpose

   Entry vector with human U6 promoter driving control Luciferase miR30-based shRNA.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Insert

   Luciferase miR-shRNA
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRFP shRNA-resistant TrkB WT

   Plasmid

   #72714

   Purpose

   Mammalian expression of TrkB fused to RFP in C-terminus, shRNA resistant

   Depositor

   Freddy Jeanneteau

   Article

   Arango-Lievano et al Proc Natl Acad Sci U S A. 2015 Nov 16. p

   Insert

   TrkB
   

   Use

   Tags

   RFP

   Expression

   Mammalian

   Mutation

   shRNA-resistant TrkB (T300C, T301A, C302G, C306A,…

   Promoter

   CMV

   Available since

   Feb. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pET:ACAT:GFP

   Plasmid

   #60740

   Purpose

   A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'ACAT' between -11 and -8.

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. V32M,L84M CoV-2 ORF8

   Plasmid

   #159934

   Purpose

   Protein overexpression in E.coli

   Depositor

   James Hurley

   Article

   Flower et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

   Insert

   CoV-2 ORF8 18-121 (ORF8 CoV-2)
   

   Use

   Tags

   6x Histidine, TEV cleavable

   Expression

   Bacterial

   Mutation

   V32M, L84M

   Promoter

   T7

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3_PKA-Cα(K8,9A)-mEGFP

   Plasmid

   #168490

   Purpose

   Mammalian expression of the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP.

   Depositor

   Haining Zhong

   Article

   Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

   Insert

   the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCNL-N1_PTS2

   Plasmid

   #65676

   Purpose

   Expresses PTS2-CNL in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   peroxisomal targeting signal 2
   

   Use

   Luciferase

   Tags

   CNL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. TAL3344

   Plasmid

   #42807

   Depositor

   Keith Joung

   Article

   Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

   Insert

   ZebrafishCommunity-prothymosin alpha a-left (ptmaa Zebrafish)
   

   Use

   t7

   Tags

   3X Flag and WT FOKI

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 19, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pGEMT-Pax7bait-T2a-Cherry-pA

    Plasmid

    #111154

    Purpose

    knock in bait for Axolotl Pax7 locus

    Depositor

    Elly Tanaka

    Article

    Fei et al Proc Natl Acad Sci U S A. 2017 Nov 21;11

    Insert

    Pax7bait-T2a-Cherry-pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pWZL blast goosecoid

    Plasmid

    #14095

    Depositor

    Bob Weinberg

    Article

    Hartwell et al Proc Natl Acad Sci U S A. 2006 Dec 12. 1

    Insert

    goosecoid (Gsc Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pRS416 Gal-RNQ1 153-405-YFP

    Plasmid

    #18689

    Depositor

    Susan Lindquist

    Article

    Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

    Insert

    Rnq1 153-405
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Prion domain of Rnq1

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

13. HL 818 (=HL 716 + pHL 98)

    Plasmid

    #30006

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Insert

    pLlacO-1:DsrA-mcherry, pLtetO-1:rpoS::gfp
    

    Use

    Synthetic Biology

    Tags

    mcherry, GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pSB74

    Plasmid

    #19591

    Depositor

    Jay Keasling

    Article

    Bang et al Appl Environ Microbiol. 2000 Sep . 66(9)

    Insert

    thiosulfate reductase (phsB S. enterica serovar Typhimurium LT2)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

15. TAL3178

    Plasmid

    #41352

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-sox2-Left (sox2 Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

16. 821 pGEX2T PTEN 1-274

    Plasmid

    #10741

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Amino acids 1-274.

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

17. D. vulgaris Cas5c/pHMGWA

    Plasmid

    #81182

    Purpose

    Expresses Desulfovibrio vulgaris Cas5c in E. coli with TEV-cleavable N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

    Insert

    Cas5c
    

    Use

    CRISPR

    Tags

    6xHis - MBP - TEV protease site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. D. vulgaris Cas8c/pHMGWA

    Plasmid

    #81183

    Purpose

    Expresses Desulfovibrio vulgaris Cas8c in E. coli with TEV-cleavable N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

    Insert

    Cas8c
    

    Use

    CRISPR

    Tags

    6xHis - MBP - TEV protease site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GFP-mROCK1-delta5

    Plasmid

    #101293

    Purpose

    Mammalian expression of Rock1-GFP for the study of localisation signaling.

    Depositor

    Alpha Yap

    Article

    Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

    Insert

    Rock1 (Rock1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    375-727 aa

    Promoter

    CMV

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYNL-C1_H2B

    Plasmid

    #65702

    Purpose

    Expresses YNL-H2B in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    histone H2B
    

    Use

    Luciferase

    Tags

    YNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. plIVMD164

    Plasmid

    #160936

    Purpose

    In vivo mRNA display construct for GFP

    Depositor

    Saeed Tavazoie

    Article

    Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

    Insert

    MCP-sfGFP-HIS tag, 3'UTR MS2 Stem Loop
    

    Use

    Tags

    HIS tag, 3'UTR MS2 Stem Loop

    Expression

    Yeast

    Mutation

    Promoter

    MET25

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Desmocollin2_tail-GST

    Plasmid

    #36992

    Depositor

    Kathleen Green

    Article

    Nekrasova et al J Cell Biol. 2011 Dec 26;195(7):1185-203

    Insert

    Dsc2 (DSC2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Intracellular domain of Dsc2 (nt 2,603–3,158)

    Promoter

    Available since

    July 31, 2012

    Availability

    Academic Institutions and Nonprofits only

23. TAL3179

    Plasmid

    #41353

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-sox2-Right (sox2 Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

24. pZF-BCB-GCN

    Plasmid

    #127476

    Purpose

    coding sequence for synthetic zinc finger ZF-BCB-GCN

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-BCB synthetic transcription factor fused with GCN4 leucine zipper
    

    Use

    Cell-free transcription–translation

    Tags

    GCN4 leucine zipper

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA5/FRT/TO/intron/FlagCter

    Plasmid

    #113548

    Purpose

    Mammalian expression plasmid that can be used with the Flp-In T-REx system.

    Depositor

    Blerta Xhemalce

    Article

    Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

    Insert

    Flag
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCDNA3_PKA-Cα(K8,9A)-mPAGFP

    Plasmid

    #168493

    Purpose

    Mammalian expression of the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

    Depositor

    Haining Zhong

    Article

    Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

    Insert

    the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMGF173

    Plasmid

    #96999

    Purpose

    NLS-GFP-lacZ leu1 insertion vector

    Depositor

    Adam Frost, Wesley Sundquist

    Article

    Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    lacZ
    

    Use

    Tags

    NLS-GFP

    Expression

    Yeast

    Mutation

    Promoter

    Pnmt1

    Available since

    Aug. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pZF-BCB-PDZ

    Plasmid

    #127478

    Purpose

    coding sequence for synthetic zinc finger ZF-BCB-PDZ

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-BCB synthetic transcription factor fused with the alpha 1-syntrophin PDZ domain
    

    Use

    Cell-free transcription–translation

    Tags

    alpha 1-syntrophin PDZ domain

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDY4

    Plasmid

    #53589

    Purpose

    Produces an active Lactuca sativa (lettuce) lycopene epsilon-cyclase in E. coli. Use with pAC-LYC or pAC-LYCipi to produce epsilon-carotene in E. coli.

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Proc Natl Acad Sci U S A. 2001 Feb 27;98

    Insert

    lcyE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. vector plix 4.03

    Plasmid

    #121130

    Purpose

    inducible expression in cells

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    empty cassete for contol of miR expression
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge