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pMA-SpCas9-g6

Plasmid

#80789

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 6/16/26 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV10-mFoxP1-ES

Plasmid

#35169

Depositor

Benjamin Blencowe

Article

Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

Insert

Foxp1 (Foxp1 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3 xNoggin

Plasmid

#24960

Depositor

Edward De Robertis

Article

Pera et al Int J Dev Biol. 2005 . 49(7):781-96.

Insert

Noggin (nog.L Frog)

Expression

Mammalian

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag Rab1a N124I

Plasmid

#46778

Purpose

expression of N124I Rab1a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab1a (Rab1a Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

N124I cannot stably bind guanine nucleotide

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pLV-EF1A-CD19.8h.8TM.28.3z

Plasmid

#200670

Purpose

Modular CD8hinge-CD8transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Michele Bernasconi

Article

Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

Insert

FMC63-CD8HD-CD8TM-CD28-CD3z-P2A-EGFP (CD8A, CD28, CD247 Human)

Use

Lentiviral

Tags

MycTag

Expression

Mammalian

Promoter

EF1a-short

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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YIplac211-yoHaloTag-TLG1

Plasmid

#105277

Purpose

Plasmid for pop-in/pop-out replacement of Tlg1 with yoHaloTag-Tlg1.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Tlg1 (TLG1 Budding Yeast)

Tags

yoHaloTag

Expression

Yeast

Mutation

HaloTag has yeast optimized codons

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt1(WT)

Plasmid

#86561

Purpose

Expresses human full-length Akt1(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Tags

10xHis

Expression

Insect

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/DEST/hCx43-E2HA-stop

Plasmid

#40909

Depositor

Robin Shaw

Article

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

Connexin 43 internal HA tag (GJA1 Human)

Expression

Mammalian

Mutation

HA tag inserted in second extracellular loop

Promoter

CMV

Available Since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO DNAJB6b

Plasmid

#19474

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB6b (DNAJB6 Human)

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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EGFP-N-Ezrin

Plasmid

#202684

Purpose

fluorescent protein tagged ezrin

Depositor

Gerry Hammond

Article

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

Mus musculus EZR(1-586):EGFP (Ezr Mouse)

Expression

Mammalian

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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NES-mTagBFP2-P4Mx1

Plasmid

#108142

Purpose

PI4P biosensor

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

X.leavis map2k1.L(32-44):mTagBFP2:L. penomophila SidM(546-647)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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Zebrafish ArcLight Q175

Plasmid

#53616

Purpose

Genetically encoded voltage sensor ArcLight with improved kinetics

Depositor

Vincent Pieribone

Article

Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

Insert

Zebrafish ArcLight-Q175 (tpte Zebrafish, Aequorea victoria, Synthetic)

Expression

Mammalian

Mutation

Dr-VSD contains R153Q mutation and an amino acid …

Promoter

CMV

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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mCherry-Sec61β

Plasmid

#172445

Purpose

Expression of ER protein Sec61beta with an N-terminal mCherry tag

Depositor

Stephen Royle

Article

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Protein transport protein Sec61 subunit beta

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pFL His-p97

Plasmid

#113510

Purpose

Expression of His-p97 in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p97 (VCP Human)

Tags

6*His

Expression

Insect

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pQE80L SpyTag-ELP-SpyTag

Plasmid

#112634

Purpose

Two SpyTags linked by an elastin-like polypeptide for hydrogel formation

Depositor

Mark Howarth

Article

Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

Insert

SpyTag-ELP-SpyTag

Tags

His6

Expression

Bacterial

Mutation

central cysteine

Promoter

T5

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pTK162_mCherry-4.1G-hardened

Plasmid

#46361

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

4.1G (EPB41L2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Modified for siRNA resistance to gcTccTcaTttCgaAc…

Promoter

CMV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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FGF10-luciferase

Plasmid

#24994

Depositor

Benoit Bruneau

Article

Agarwal et al Development. 2003 Feb . 130(3):623-33.

Insert

Fgf10 promoter (Fgf10 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
pET28a-cMSP26

Plasmid

#199769

Purpose

Expresses split-intein fused MSP26 for circularization during expression (internal His-Tag)

Depositor

Franz Hagn

Article

Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

Insert

cMSP26

Tags

His6-Tag

Expression

Bacterial

Mutation

internal His-Tag

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pDendra2-H3

Plasmid

#157814

Purpose

Fluorescent reporter for histone H3 tracking

Depositor

Xin Chen

Article

Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

Insert

H3 (H3c1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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p006-Banana-Late

Plasmid

#112251

Purpose

ATF1 from Saccharomyces cerevisiae under osmY stationary phase promoter; kanamycin resistant.

Depositor

Jaroslaw Bryk

Article

Siddall et al bioRxiv 2021.06.20.449138

Insert

ATF1 under osmY promoter

Expression

Bacterial

Promoter

osmY

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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