Addgene

We narrowed to 17,056 results for: URE

Showing: 1861 - 1880 of 17056 results

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mCBFKOgRNA5

Plasmid

#84366

Purpose

Cas9-gRNA plasmid for mouse Cbfa2t2 Knockout

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

Cbfa2t2-gRNA5

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
mCBFKOgRNA2

Plasmid

#84365

Purpose

Cas9-gRNA plasmid for mouse Cbfa2t2 Knockout

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

Cbfa2t2-gRNA2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
pCMV-EPN-01*-(ΔLF)

Plasmid

#80045

Purpose

Expression of Vpr binding mutant EPN-01*

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01*(L33R)

Use

Tags

Myc

Expression

Mammalian

Mutation

Vpr binding site LF45 in p6 mutated

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01(ΔI)

Plasmid

#80048

Purpose

Expression of EPN-01 assembly mutant L33R

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01(L33R)

Use

Tags

Myc

Expression

Mammalian

Mutation

L33R

Promoter

CMV

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01(ΔL1+L2)

Plasmid

#80051

Purpose

Expression of EPN-01 mutated in both L domains

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01(deltaPTAP/deltaYP)

Use

Tags

Myc

Expression

Mammalian

Mutation

PTAP10AAAA, YP37AA

Promoter

CMV

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01*(ΔI)

Plasmid

#80041

Purpose

Expression of EPN-01 assembly mutant L33R

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01*(L33R)

Use

Tags

Myc

Expression

Mammalian

Mutation

changed Leu33 in I3-01 to Ala

Promoter

CMV

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pASKt15C+SrtA2woH

Plasmid

#65018

Purpose

Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Use

Tags

S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K, E108A

Promoter

tetracycline promoter/operator

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pUASTattb AaegOr4-G

Plasmid

#59798

Purpose

UAS-induced expression of Aedes aegypti odorant receptor 4 (OR4) cDNA allele G

Depositor

Leslie Vosshall

Article

McBride et al Nature. 2014 Nov 13;515(7526):222-7. doi

Insert

Aedes aegypti Or4 cDNA allele G

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pUASTattb AaegOr4-F

Plasmid

#59797

Purpose

UAS-induced expression of Aedes aegypti odorant receptor 4 (OR4) cDNA allele F

Depositor

Leslie Vosshall

Article

McBride et al Nature. 2014 Nov 13;515(7526):222-7. doi

Insert

Aedes aegypti Or4 cDNA allele F

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pUASTattb AaegOr4-D

Plasmid

#59795

Purpose

UAS-induced expression of Aedes aegypti odorant receptor 4 (OR4) cDNA allele D

Depositor

Leslie Vosshall

Article

McBride et al Nature. 2014 Nov 13;515(7526):222-7. doi

Insert

Aedes aegypti Or4 cDNA allele D

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pUASTattb AaegOr4-B

Plasmid

#59793

Purpose

UAS-induced expression of Aedes aegypti odorant receptor 4 (OR4) cDNA allele B

Depositor

Leslie Vosshall

Article

McBride et al Nature. 2014 Nov 13;515(7526):222-7. doi

Insert

Aedes aegypti Or4 cDNA allele B

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a-MGGG-ybbr-HIS-SdrD_B1-DK

Plasmid

#117983

Purpose

SdrD B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB

Depositor

Hermann Gaub

Article

Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

Insert

SdrG_B2

Use

Tags

HIS, sortase, and ybbr

Expression

Bacterial

Mutation

Promoter

T7

Available since

Availability

Academic Institutions and Nonprofits only

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pAW

Plasmid

#133893

Purpose

Expression of Gal4AD-Prey hybrid protein

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pBW

Plasmid

#133897

Purpose

Expression of Gal4BD-Bait hybrid protein.

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pSIRV-NF-kB-eCFP

Plasmid

#118094

Purpose

Flourescent reporter for NF-kB activity

Depositor

Peter Steinberger

Article

Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

Insert

eCFP

Use

Retroviral

Tags

Expression

Mutation

Promoter

minimal promoter with NF-kB responsive element

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pET30b_OmpAT7Sav

Plasmid

#138589

Purpose

T7-tagged streptavidin with native C-terminus and periplasmic export signal OmpA; controlled by PT7 promoter; KanR, pBR322 ori

Depositor

Markus Jeschek

Article

Jeschek et al Nature. 2016 Sep 29;537(7622):661-665. d

Insert

T7-tagged streptavidin variant with native C-terminus and OmpA signal peptide for periplasmic export

Use

Synthetic Biology

Tags

OmpA export signal and T7 tag

Expression

Bacterial

Mutation

Promoter

PT7

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_Lambda-N-HA-Peptide

Plasmid

#92005

Purpose

Expresses Lambda-N-HA peptide

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

Lambda-N-HA peptide

Use

Tags

Lambda-N-HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-DTR-GFP

Plasmid

#124364

Purpose

conditional expression of a diphtheria toxin receptor (DTR)–GFP fusion protein

Depositor

Eiman Azim, Thomas Jessell

Article

Azim et al Nature. 2014 Apr 17;508(7496):357-63. do

Has Service

AAV2

Insert

DTR-GFP

Use

AAV

Tags

GFP

Expression

Mutation

Promoter

Chicken B-actin

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAcGFP-MALAT1M

Plasmid

#120382

Purpose

Reporter for RNA nuclear localization

Depositor

Igor Ulitsky

Article

Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

Insert

MALAT1 (MALAT1 Human)

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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PMXS-NDI1

Plasmid

#72876

Purpose

expresses NDI1 in mammalian cells

Depositor

David Sabatini

Article

Birsoy et al Nature. 2014 Apr 3;508(7494):108-12. doi

Insert

NDI1 (NDI1 Budding Yeast)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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