We narrowed to 7,177 results for: cas9 plasmid

1. pLC133

   Plasmid

   #198639

   Purpose

   Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.

   Depositor

   David Bikard

   Article

   Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

   Insert

   dCas9
   

   Use

   Tags

   3x-FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pST_119_LVL2 cam

   Plasmid

   #179333

   Purpose

   NT-CRISPR plasmid for integration of multiple gRNAs.

   Depositor

   Anke Becker

   Article

   Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

   Insert

   Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, sfGFP dropout to be replaced with gRNAs
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. eGFPbait-E2A-KalTA4-pA donor vector

   Plasmid

   #61069

   Purpose

   for CRISPR/Cas9 mediated insertion of E2A-KalTA4; to be used in combination with a eGFP specific sgRNA (e.g. Plasmid 61051)

   Depositor

   Filippo Del Bene

   Article

   Auer et al Genome Res. 2014 Jan;24(1):142-53. doi:

   Inserts

   eGFPbait
   KalTA4
   

   Use

   Zebrafish expression

   Tags

   E2A

   Expression

   Mutation

   Promoter

   Available since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHRdSV40_scFv_GCN4_sfGFP-VPR-GB1_NLS

   Plasmid

   #79373

   Purpose

   Recruits VPR to a compatible Cas9 protein for transcriptional activation

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   GCN4-sfGFP-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHRdSV40_scFv_GCN4_sfGFP_p65-hsf1_GB1_NLS

   Plasmid

   #79372

   Purpose

   Recruits p65-hsf1 to a compatible Cas9 protein for transcriptional activation

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   GCN4-sfGFP-p65-hsf1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSP2283

   Plasmid

   #70702

   Purpose

   Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. L40C-CRISPR.EFS.PAC

   Plasmid

   #89393

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), PAC (Puromycin resistance) coexpression, EFS Promoter driven

   Depositor

   Dirk Heckl

   Article

   Reimer et al Haematologica. 2017 Jun 1. pii: haematol

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSP2266

   Plasmid

   #70705

   Purpose

   Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCY5.1nh

   Plasmid

   #160299

   Purpose

   Yeast CRISPR plasmid targeting the natMX and hphMX cassettes

   Depositor

   Amir Aharoni

   Article

   Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

   Inserts

   sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
   URA3MX selection cassette
   sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
   Cas9
   

   Use

   CRISPR

   Tags

   8xHis-tag and SV40 NLS

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCY5.1kn

    Plasmid

    #160298

    Purpose

    Yeast CRISPR plasmid targeting the kanMX and natMX cassettes

    Depositor

    Amir Aharoni

    Article

    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

    Inserts

    sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
    URA3MX selection cassette
    sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
    Cas9
    

    Use

    CRISPR

    Tags

    8xHis-tag and SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMTF1.1.0-gDNA

    Plasmid

    #113797

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MTF1

    Depositor

    Kevin White

    Insert

    MTF1 (MTF1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZBTB9.1.0-gDNA

    Plasmid

    #112469

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZBTB9

    Depositor

    Kevin White

    Insert

    ZBTB9 (ZBTB9 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZBTB5.1.0-gDNA

    Plasmid

    #112399

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZBTB5

    Depositor

    Kevin White

    Insert

    ZBTB5 (ZBTB5 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZNF436.1.0-gDNA

    Plasmid

    #113767

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF436

    Depositor

    Kevin White

    Insert

    ZNF436 (ZNF436 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZFX.1.0-gDNA

    Plasmid

    #112462

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZFX

    Depositor

    Kevin White

    Insert

    ZFX (ZFX Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMXD1.1.0-gDNA

    Plasmid

    #112446

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MXD1

    Depositor

    Kevin White

    Insert

    MXD1 (MXD1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUSF2.1.0-gDNA

    Plasmid

    #112457

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor USF2

    Depositor

    Kevin White

    Insert

    USF2 (USF2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZNF331.1.0-gDNA

    Plasmid

    #112461

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF331

    Depositor

    Kevin White

    Insert

    ZNF331 (ZNF331 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVEZF1.1.0-gDNA

    Plasmid

    #112437

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor VEZF1

    Depositor

    Kevin White

    Insert

    VEZF1 (VEZF1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pZNF416.1.0-gDNA

    Plasmid

    #112468

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF416

    Depositor

    Kevin White

    Insert

    ZNF416 (ZNF416 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge