We narrowed to 65,691 results for: nin

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Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1

Plasmid

#138460

Purpose

lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRAB

Depositor

Jian Xu

Article

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

MCP-KRAB-IRES-zsGreen1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBAMD1-4

Plasmid

#61565

Purpose

Standardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin resistant

Depositor

Víctor de Lorenzo

Article

Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

Type

Empty backbone

Use

Synthetic Biology; Mini-tn5 vector

Available Since

Jan. 7, 2015

Availability

Academic Institutions and Nonprofits only

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Flag-HA-EIF3S3

Plasmid

#22545

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

EIF3S3(1-348aa) (EIF3H Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-nv5-ER beta

Plasmid

#22770

Depositor

Donald McDonnell

Article

Wittmann et al Cancer Res. 2007 Oct 1. 67(19):9549-60.

Insert

estrogen receptor beta (ESR2 Human)

Tags

v5

Expression

Mammalian

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

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GFP-PS9

Plasmid

#177944

Purpose

Transient mammalian expression of eGFP along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

eGFP (ORF1ab )

Expression

Mammalian

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-ccdB

Plasmid

#14147

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Gateway Cloning

Expression

Yeast

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G

Plasmid

#177960

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )

Expression

Mammalian

Mutation

D614G

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-RET

Plasmid

#23906

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RET (RET Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pEF1a-mMLH1dn

Plasmid

#174825

Purpose

Mammalian expression of mouse MLH1dn (codon optimized)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

mouse MLH1dn (codon optimized)

Expression

Mammalian

Mutation

deletion of residues 758-760

Promoter

EF1a

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCF CREB M1

Plasmid

#22969

Depositor

Marc Montminy

Article

Du et al Mol Cell Biol. 2000 Jun . 20(12):4320-7.

Insert

CREB (Creb1 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

Changed Serine 133 to Alanine

Available Since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D-TurboID(full)-HA-GPI

Plasmid

#166055

Purpose

Expresses the full length TurboID on the surface of astrocytes

Depositor

Scott Soderling

Article

Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

Insert

GPI anchored TurboID

Use

AAV

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-SPI1

Plasmid

#97039

Purpose

Expresses SPI1 in mammalian cells

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Spi-1 Proto-Oncogene (SPI1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only
pCMV-Neo-Bam APC 1-1309

Plasmid

#16509

Depositor

Bert Vogelstein

Article

Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

Insert

APC (APC Human)

Expression

Mammalian

Mutation

Codon 1309 truncation

Available Since

April 30, 2008

Availability

Academic Institutions and Nonprofits only
pET42b-HF-BE3

Plasmid

#87438

Purpose

Expresses HF-BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

Depositor

David Liu

Article

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

HF-BE3 (Apobec1 Bacillus phage PBS2, Human, Rat)

Tags

His6

Expression

Bacterial

Available Since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-U6-dgRNA-CAG-MPH

Plasmid

#106259

Purpose

Expresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backbone

Depositor

Juan Belmonte

Article

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Insert

MS2-P65-HSF1 (HSF1 Human, Synthetic)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-SF2

Plasmid

#99021

Purpose

Mammalian expression of SF2

Depositor

Honglin Chen

Article

Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

Insert

SF2 (SRSF1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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Nanog-2A-mCherry

Plasmid

#59995

Purpose

Inserts mCherry immediately downstream of the Nanog coding region

Depositor

Rudolf Jaenisch

Article

Faddah et al Cell Stem Cell. 2013 Jul 3;13(1):23-9. d

Insert

Nanog (Nanog Mouse)

Use

Mouse Targeting

Tags

2A-mCherry

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR-AIF

Plasmid

#16182

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Apoptosis-inducing factor (AIFM1 Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only

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pEJS614_pTetR-P2A-BFPnls/sgNS

Plasmid

#108650

Purpose

Non-specific Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Non-specific sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Promoter

U6 and hPGK

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pLARRY-EGFP

Plasmid

#140025

Purpose

Lentiviral backbone for cloning expressed barcodes in the 3'UTR of the EGFP mRNA

Depositor

Fernando Camargo

Article

Weinreb et al Science. 2020 Jan 23. pii: science.aaw33

Insert

EGFP

Use

Lentiviral

Promoter

EF1alpha

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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