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Showing: 3761 - 3800 of 8751 results

  6. PB-CT3G-CERP2-MG

    Plasmid
    #175503
    Purpose
    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G-mCMV
    Available since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175503-plasmid-map-sequence-id-345096

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  9. pRDA_118

    Plasmid
    #133459
    Purpose
    U6 promoter expresses customizable Spyo-guide; EF1a promoter provides puromycin resistance. This vector is a derivative of the lentiGuide vector, with minor modifications to the tracrR
    Depositor
    John Doench
    Article
    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1
    Insert
    control guide
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    133459-plasmid-map-sequence-id-261403

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  12. pDIV643

    Plasmid
    #226182
    Purpose
    Expresses a color marker (uidA) cassette for disruption on KU70 gene in K. phaffii.
    Depositor
    Uffe Mortensen
    Article
    Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi
    Insert
    uidA marker flanked with targeting regions to KU70 gene in K.phaffii
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    TEF1p
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226182-plasmid-map-sequence-id-449156

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  19. pFC330

    Plasmid
    #87842
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9 and pyrG selection marker
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    pyrG
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans tef1 promoter and native pro…
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    87842-plasmid-map-sequence-id-189665

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  26. pDual-eGFP

    Plasmid
    #63215
    Purpose
    Expression of eGFP in bacteria and in mammalian cells. Used as a reporter for quantifying gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Article
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV-EF1α hybrid (CEF)
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only

  27. pFUSE-rabbitFc1-IL2ss-VHH(G08)-His-Myc

    Plasmid
    #209857
    Purpose
    Plasmid coding for a nanobody (termed G08) against FGFR3 (mus musculus) with x6 HisTag, x3 myc, and Fabbit FC gamma 1
    Depositor
    Tim Brown
    Inserts
    IL2-ss
    VHH (G08 variant)
    Rabbit Fc Gamma 1
    Use
    Tags
    x3 MycTag and x6 HisTag
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1-HTLV promoter
    Available since
    Nov. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    209857-plasmid-map-sequence-id-414817

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  29. pCSIIhyg-rtTA

    Plasmid
    #139480
    Purpose
    Expresses rtTA, Tet-ON reverse tetracycline-controlled trans-activator in mammalian cells.
    Depositor
    Kazuhiro Aoki
    Article
    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2
    Insert
    rtTA, Tet-ON reverse transactivator
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human EF1a promoter
    Available since
    March 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    139480-plasmid-map-sequence-id-276014

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  33. PB-CT3G-ERP2-MG

    Plasmid
    #175501
    Purpose
    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G-mCMV
    Available since
    Oct. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    175501-plasmid-map-sequence-id-344442

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  39. pAC1430

    Plasmid
    #127981
    Purpose
    AMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
    Insert
    Cpf1
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    codon optimized for Aspergillus nidulans
    Promoter
    Aspergillus nidulans tef1 promoter
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    127981-plasmid-map-sequence-id-254494

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Showing: 3761 - 3800 of 8751 results