We narrowed to 2,475 results for: T2A

1. pCBH-Cas9-Suntag-BB_BbsI

   Plasmid

   #164805

   Purpose

   REDIT backbone for SunTag Cas9, pU6-gRNA-backbone(BbsI)-CBH-SpCas9-Suntag-T2A-EBFP

   Depositor

   Le Cong

   Article

   Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Antares-SEPLuc C1A4E

   Plasmid

   #141088

   Purpose

   precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES and weaker precursor of Nanoluc

   Depositor

   Jeak Ling Ding

   Article

   Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

   Insert

   Antares-T2A-puromycin-IRESv24-SEP-C1A4E
   

   Expression

   Mammalian

   Mutation

   Please see depositor comments

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK360

   Plasmid

   #71571

   Purpose

   Produces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase (UctC) with Thr2>Ala mutant (UctC-T2A)

   Depositor

   T. J. Kappock

   Article

   Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

   Insert

   acetyl-CoA:oxalate CoA-transferase
   

   Expression

   Bacterial

   Mutation

   changes threonine-2 to alanine

   Promoter

   T7

   Available Since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDG459

   Plasmid

   #100901

   Purpose

   SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

   Depositor

   Paul Thomas

   Article

   Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

   Inserts

   humanized CRISPR associated protein 9
   U6-gRNA scaffold 1
   U6-gRNA scaffold 2
   

   Use

   CRISPR and Mouse Targeting

   Tags

   3xFLAG and T2A-PuroR

   Expression

   Mammalian

   Promoter

   CBh and U6

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRosetta_v2

   Plasmid

   #136477

   Purpose

   Lentiviral GFP Reporter assay for determining appropriate dose of puromycin, blasticidin, and hygromycin

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

   Insert

   HygroR-T2A-BlastR-P2A-PuroR-F2A-EGFP
   

   Use

   Lentiviral

   Promoter

   PGK

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLCN DSB Repair Reporter (DRR)

   Plasmid

   #98895

   Purpose

   DSB Repair Reporter (DRR). Expresses GFP after cut of two inverted ISce1 sites and repair by NHEJ.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   Neomycin-T2A-ISce1-ISce1-eGFP
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. CuO-LacI-NLS–SNAPf

   Plasmid

   #227368

   Purpose

   Lentiviral expression of LacI–SNAP

   Depositor

   David Pellman

   Article

   Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

   Insert

   LacI NLS SNAPf
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. S1m tagged DANCR

   Plasmid

   #138585

   Purpose

   For in vivo tagging

   Depositor

   Yin-Yuan Mo

   Article

   Hu et al Cell Death Differ. 2019 Dec 5. pii: 10.1

   Insert

   DANCR (DANCR Human)
   

   Use

   Lentiviral

   Tags

   S1m tag

   Promoter

   MSCV

   Available Since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMK1334

   Plasmid

   #127965

   Purpose

   sgRNA expression vector compatible with CROP-Seq and imaging screens

   Depositor

   Martin Kampmann

   Article

   Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

   Insert

   EF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Aug. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Caspase-Reporter

    Plasmid

    #243673

    Purpose

    Fluorescent Reporter for Caspase 3/7 activity. Contains DEVD sequence.

    Depositor

    Matthias Wirth

    Article

    Selcen et al Cell Death Discov, 11, 368, 06 August 20

    Insert

    Split-GFP with DEVD sequence and T2A mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLQ1371-sgSTAT3-1

    Plasmid

    #121425

    Purpose

    sgSTAT3-1 sequence: GTCAGGATAGAGATAGACCAG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

    Depositor

    Stanley Qi, Ovijit Chaudhuri

    Article

    Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

    Insert

    sgSTAT3-1
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Iron Insensitive Control Construct 2

    Plasmid

    #243007

    Purpose

    Iron Insensitive Control Construct under the same expression promoter used in IronFist

    Depositor

    Roland Malli

    Article

    Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

    Inserts

    mNeonGreen
    T2A
    mCherry
    

    Use

    Gateway

    Expression

    Mammalian

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHPHS853

    Plasmid

    #228236

    Purpose

    Bxb1 attB_GA mCherry-T2A-puro, Dox-inducible EYFP reporter for integration into Bxb1 attP_GA landing pad

    Depositor

    Arvind Rasi Subramaniam

    Article

    Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d

    Inserts

    mCherry
    EYFP
    

    Tags

    HA and PuroR

    Expression

    Mammalian

    Promoter

    TRE3GV and none

    Available Since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAVS1-Puro-CAG-GCaMP6s

    Plasmid

    #120896

    Purpose

    Donor plasmid targeting human AAVS1 safe harbor locus to generate GCaMP6s gene knockin. Contains two homologous arms flanking T2A-Puro resistance gene and CAG-driven GCaMP6s gene.

    Depositor

    Xiaojun Lian

    Article

    Jiang et al iScience. 2018 Oct 12;9:27-35. doi: 10.1

    Insert

    GCaMP6s
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EFS-CjCas9-eGFP-HIF1a

    Plasmid

    #137929

    Purpose

    Expression of cjCas9 with Hif1a targeting sgRNA

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

    Insert

    CjCas9
    

    Use

    AAV and CRISPR

    Tags

    SV40NLS-HA-T2A-EGFP

    Promoter

    EF-1alpha

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQAT27

    Plasmid

    #223399

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by 2x35s and the sgRNA was driven by AtU3; Kanamycin for plant select.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-HNF4A-FOXA2-FOXA3

    Plasmid

    #149724

    Purpose

    expressing three liver promoting transcription factors (HNF4A, FOXA2, FOXA3)

    Depositor

    Baisong Lu

    Article

    Lu et al Biomaterials. 2021 Feb;269:120668. doi:

    Insert

    HNF4A-FOXA2-FOXA3
    

    Use

    Lentiviral

    Tags

    P2A, T2A

    Promoter

    CMV

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQAT25

    Plasmid

    #223397

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by AtUBQ10 and sgRNA was driven by AtU3; Hygromycin for plants select.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    AtUBQ10-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-pEF1a-tTAc

    Plasmid

    #172123

    Purpose

    Constitutive expression of InteinC-tTAC

    Depositor

    Chun Xu

    Article

    Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

    Insert

    InteinC-tTAc
    

    Use

    AAV

    Promoter

    EF1a

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLGR002 LGR Cas9 guide vector

    Plasmid

    #188320

    Purpose

    Universal backbone for CRISPR/Cas9 sgRNA expression

    Depositor

    Laralynne Przybyla

    Article

    Heo et al Genome Biol. 2024 Jan 19;25(1):25. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    PuroR-T2A-mTagBFP2

    Expression

    Mammalian

    Promoter

    pU6 5'CMV and EF1-alpha

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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