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VCP(R155H)-EGFP

Plasmid

#23972

Depositor

Nico Dantuma

Article

Tresse et al Autophagy. 2010 Feb 22. 6(2):.

Insert

Valosin containing protein (VCP Human)

Tags

6xHis, EGFP, and HA

Expression

Mammalian

Mutation

R155H

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

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pk7-GR-GFP

Plasmid

#15534

Depositor

Ian Macara

Article

Carey et al J Cell Biol. 1996 Jun . 133(5):985-96.

Insert

Glucocorticoid receptor (NR3C1 Human)

Tags

GFP

Expression

Mammalian

Mutation

The glucocorticoid receptor was cloned into the X…

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

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pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE

Plasmid

#20945

Purpose

3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Use

Lentiviral

Expression

Mammalian

Mutation

H134R mutation in ChR2

Promoter

hSyn

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

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Human SHIP1 (clone hp51CN)

Plasmid

#123347

Purpose

Expression of human SHIP1

Depositor

Christophe Erneux

Article

Drayer et al Biochem Biophys Res Commun. 1996 Aug 5;2

Insert

INPP5D (INPP5D Human)

Expression

Mammalian

Available Since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-Tol1.zf1

Plasmid

#61388

Purpose

mRNA synthesis of tol1 transposase codon modified for zebrafish

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Insert

tol1.zf1

Use

Zebrafish expression

Promoter

sp6

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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AiP14132 - pAAV-AiE0779m_3xC2-minBG-jGCaMP8m-WPRE3-BGHpA (Alias: CN4132)

Plasmid

#191735

Purpose

AI plasmid ALIAS: AiP14132. Expression of jGCaMP8m in striatal direct pathway medium spiny neurons (D1-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

jGCaMP8m

Use

AAV

Mutation

none

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsXRN1

Plasmid

#66596

Purpose

expresses lambdaN-HA-tagged XRN1 in mammalian cells

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

XRN1 (XRN1 Human)

Tags

lambdaN-HA

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pACBSR-hyg

Plasmid

#87830

Purpose

Expresses lambda-red system with hyg selection marker

Depositor

Pep Charusanti

Article

Huang et al BMC Res Notes. 2014 Jan 8;7:13. doi: 10.

Insert

hyg

Tags

None

Expression

Bacterial

Mutation

None

Promoter

bla

Available Since

Aug. 5, 2017

Availability

Academic Institutions and Nonprofits only

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mEmerald-Integrin-Beta1-N-18

Plasmid

#54129

Purpose

Localization: Integrin/Focal Adhesions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Integrin-Beta1 (ITGB1 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3 CDK7 HA (P#633)

Plasmid

#14647

Depositor

Matija Peterlin

Article

Kanazawa et al Oncogene. 2003 Aug 28. 22(36):5707-11.

Insert

Cdk7 (CDK7 Human)

Tags

HA

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pLIX403_RPS2_APOBEC_HA_P2A_mRuby_Capture1

Plasmid

#194703

Purpose

Inducible lentiviral expression, TRE-RPS2-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA (Ribo-STAMP, RPS2)

Depositor

Eugene Yeo

Article

Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

Insert

RPS2 (RPS2 Human)

Use

Lentiviral

Tags

APOBEC1-HA-P2A-mRuby

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-i53

Plasmid

#92170

Purpose

recombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant (i53) that is a selective inhibitor of 53BP1

Depositor

Daniel Durocher

Article

Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

Insert

i53

Use

AAV

Tags

FLAG

Expression

Mammalian

Mutation

UbvG08 with I44A mutation and no terminal Glycines

Promoter

CMV

Available Since

July 13, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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GFP scFv [N86/38]

Plasmid

#182095

Purpose

Mammalian Expression of GFP scFv. Derived from hybridoma N86/38.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting GFP

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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Jun lab tunable CRISPRi strain

Bacterial Strain

#86400

Purpose

Strain SJ_XTL219 Genotype: MG1655 PBAD_dcas9 ΔlacI ΔaraE araFGH<>spec lacY A177C, galM<pBBa-J23119-tet-sacB-handle-term

Depositor

Suckjoon Jun

Article

Li et al Sci Rep. 2016 Dec 20;6:39076. doi: 10.10

Bacterial Resistance

Spectinomycin and hygromycin

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
MSCV-mir-17-92

Plasmid

#64100

Purpose

expresses mir-17-92

Depositor

Lin He

Article

Olive et al Elife. 2013 Oct 15;2:e00822. doi: 10.755

Insert

miR-17-92 (MIR17HG Human)

Expression

Mammalian

Promoter

MSCV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pYS29

Plasmid

#202086

Purpose

Parent vector for producing universal minicircle donors for HaloTag N-terminal knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

NT HygR-T2A-Halo Minicircle Parent

Use

CRISPR

Tags

HaloTag

Promoter

None

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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TRE-KRAB-dCas9-IRES-GFP

Plasmid

#85556

Purpose

2nd generation lentiviral vector expressing a KRAB-dCas9 fusion protein and GFP

Depositor

Eric Lander

Article

Fulco et al Science. 2016 Sep 29. pii: aag2445.

Insert

KRAB-dCas9-IRES-GFP

Use

CRISPR and Lentiviral

Tags

2x NLS, HA Tag, and KRAB domain

Expression

Mammalian

Promoter

TRE3G

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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qTAG-C-miRFP670nano3-Blast-H2BC11

Plasmid

#207762

Purpose

Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

H2BC11 Homology Arms flanking a miRFP670nano3-Blast Cassette (H2BC11 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 29, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-CAG-RLuc

Plasmid

#83282

Purpose

recombinant AAV vector packaging single-stranded Renilla Luciferase under the CAG promoter (includes WPRE element)

Depositor

Mark Kay

Insert

Renilla Luciferase

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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Flag Nprl2 pRK5

Plasmid

#46333

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Nprl2 (NPRL2 Human)

Tags

Flag

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pET21a-Traptavidin

Plasmid

#24753

Depositor

Mark Howarth

Article

Chivers et al Nat Methods. 2010 May;7(5):391-3. doi: 1

Insert

Traptavidin

Tags

His-6

Expression

Bacterial

Mutation

Streptavidin with 2 amino acid mutations: S52G an…

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

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pPalmitoyl-mTurquoise2

Plasmid

#36209

Purpose

In vivo visualization of the membrane (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

p63(1-29) (ARHGEF25 Human)

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CamKII 0.4.Cre.SV40

Plasmid

#105558

Purpose

AAV expression of Cre from CamKII promoter

Depositor

James M. Wilson

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

Cre

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CamKII

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-HA-ATAD3A-IRES-Hygro

Plasmid

#211469

Purpose

Lentivirus backbone expressing ATAD3A with an HA-tag at the N-terminal

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ATAD3A (ATAD3A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EF-1a

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-PDGFRA

Plasmid

#116769

Purpose

Lentiviral expression of PDGFRA

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PDGFRA (PDGFRA Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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LifeAct-GFP-Fis1

Plasmid

#182576

Purpose

Expresses LifeAct-GFP targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence

Depositor

Uri Manor

Article

Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

Insert

LifeAct-GFP-Fis1

Tags

Fis1 and LifeAct

Expression

Mammalian

Available Since

April 7, 2022

Availability

Academic Institutions and Nonprofits only

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LARRYv2-EGFP library 2

Pooled Library

#233216

Purpose

Barcoding pooled library

Depositor

Alejo Rodriguez-Fraticelli

Article

Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

Species

Synthetic

Use

Lentiviral

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only
pT3-EF1aH NICD1

Plasmid

#86500

Purpose

Mammalian expression of Myc tagged NICD1

Depositor

Xin Chen

Article

Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

Insert

Notch1 (C-term) (Notch1 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

Cytoplasmic domain only (AA 1753-2531)

Promoter

EF1a

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab5A Q79L

Plasmid

#28046

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rab5A (RAB5A Human)

Tags

EGFP on N-term

Expression

Mammalian

Mutation

Q79L

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cyto-ExRai-AktAR2

Plasmid

#184048

Purpose

Cytosol-targeted ExRai-AktAR2.

Depositor

Jin Zhang

Article

Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

Insert

Cyto-ExRai-AktAR2

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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