We narrowed to 7,609 results for: trac

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pCFJ2369 - intron GG1 - 250bp

Plasmid

#159877

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG1 - 250bp

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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tol2-mpx-lyn-GFP

Plasmid

#35037

Depositor

Anna Huttenlocher

Article

Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

Insert

Lyn-GFP (lyn Zebrafish)

Use

Zebrafish expression

Tags

GFP

Expression

Mammalian

Available Since

March 5, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-EFS-DIO-GRAB_ATP1.0

Plasmid

#167581

Purpose

Express the ATP sensor GRAB_ATP1.0 in Cre positive cells

Depositor

Yulong Li

Article

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP sensor GRAB_ATP1.0 (P2RY1 Human)

Use

AAV

Promoter

EFS

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10

Plasmid

#198450

Purpose

Produces PVCs retargeted with VHH7 anti-mouse MHC class II nanobody; also contains truncated spike tip protein (Pvc10).

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10

Expression

Bacterial

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLV.UBI.GFP.miR292T

Plasmid

#115972

Purpose

miR-292 regulated GFP expression

Depositor

Johan Jakobsson

Article

Sachdeva et al Proc Natl Acad Sci U S A. 2010 Jun 22;10

Insert

GFP.miR292T

Use

Lentiviral

Tags

GFP

Promoter

ubiquitin

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Lifeact-(GGS)X6-HaloTag C58

Plasmid

#171435

Purpose

Expression of Lifeact-(GGS)X6-HaloTag C58 in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

Lifeact-(GGS)X6-HaloTag C58

Expression

Mammalian

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLV(cmv)-NEO-FSF-tdTomato Cre reporter

Plasmid

#160551

Purpose

labeling hypoxic cells, Cre reporter

Depositor

Marc Vooijs

Article

Vermeer et al Dis Model Mech. 2020 Jul 30;13(7). pii:

Insert

tdTomato

Use

Lentiviral

Promoter

CMV

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc12-HiBiT

Plasmid

#198308

Purpose

Produces PVCs tagged with HiBiT on the baseplate; used as assembly/loading control for payload loading assays

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc12-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pN-PITCh-3XHA

Plasmid

#127881

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HA tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-3XHA (PPP2CA Human)

Use

CRISPR

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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hBACCS2

Plasmid

#67625

Purpose

Mammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

hBACCS2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pBluescriptSKII+ U6-sgRNA(F+E) ACTB

Plasmid

#74705

Purpose

Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of ACTB mRNA

Depositor

Eugene Yeo

Article

Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

Insert

U6 promoter driving sgRNA targeting the 3'UTR of ACTB mRNA

Use

CRISPR

Expression

Mammalian

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#112924

Purpose

piggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant marker

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo

Use

Piggybac transposon vector

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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delCMV-mCherry

Plasmid

#214143

Purpose

Control sensor

Depositor

Leif Dehmelt

Article

Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

Insert

mCherry

Expression

Mammalian

Promoter

delCMV

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCFJ2092 - [1-2] ENTR - Fluor - mNeon(PATC)

Plasmid

#159853

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - mNeon(PATC)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pPy-CAG-Cre::ERT2-IRES-BSD

Plasmid

#48760

Purpose

CAG promoter driving Cre-ERT2 fusion-IRES-Blasticidin resistance

Depositor

Austin Smith

Article

Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

Insert

Cre::ERT2

Use

Ppy

Tags

ERT2

Expression

Mammalian

Promoter

CAG

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-Lifeact-miRFP703

Plasmid

#178530

Purpose

Expresses Lifeact-miRFP703 in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

Lifeact-miRFP703

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pGAN230

Plasmid

#131122

Purpose

reverse yTRAP, RNQ1-aTF reporting on tetR-mKate2

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Expression

Yeast

Promoter

SUP35

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMM441

Plasmid

#61271

Purpose

Also referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.

Depositor

Timothy Lu

Article

Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

Insert

Cas9, tracrRNA, crRNA

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-jRex-GECO

Plasmid

#169259

Purpose

AAV transfer plasmid for neuronal expression of jRex-GECO.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

JRex-GECO

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pPI138

Plasmid

#113229

Purpose

extrachromosomal expression vector, expression of LifeAct-GFP

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

LifeAct

Use

Dictyostelium discoideum expression vector, extra…

Tags

GFP

Promoter

act15

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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