233,421 results

1. pCAG-hCas9

   Plasmid

   #51142

   Purpose

   Expresses hCas9 under the CAG promoter for CRISPR

   Depositor

   Izuho Hatada

   Article

   Horii et al Int J Mol Sci. 2013 Sep 30;14(10):19774-

   Insert

   hCas9
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pCW57-GFP-2A-Cas9

   Plasmid

   #170805

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of Cas9 in combination with turbo GFP using the P2A self-cleaving peptide

   Depositor

   Morten Frodin

   Article

   Niu et al Nat Genet. 2022 Dec 5. doi: 10.1038/s415

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS420

   Plasmid

   #11259

   Depositor

   Fred Cross

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKS diaCas9_sgRNA

   Plasmid

   #74923

   Purpose

   Expresses Cas9 codon optimized for Phaeodactylum tricornutum and a sgRNA driven by a U6 promoter

   Depositor

   Per Winge

   Article

   Nymark et al Sci Rep. 2016 Apr 25;6:24951. doi: 10.10

   Inserts

   LHCF2 promoter
   diaCas9
   LHCF1 terminator
   U6 promoter
   sgRNA
   U6 3' region
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Cas9 module and sgRNA module

   Available since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223-CSNK1A1

   Plasmid

   #23355

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   CSNK1A1 (CSNK1A1 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pAAV.CMV.LacZ.bGH

   Plasmid

   #105531

   Purpose

   AAV expression of LacZ from CMV promoter

   Depositor

   James M. Wilson

   Has Service

   AAV5 and AAV8

   Insert

   LacZ
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTriEx-RhoA-wt_mScarlet-i_SGFP2

   Plasmid

   #85071

   Purpose

   Green/red FRET biosensor to monitor RhoA nucleotide loading state and activity

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   RhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)
   

   Use

   Tags

   6xHis and mScarlet-i and SGFP2

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDGE334

   Plasmid

   #153243

   Purpose

   M2E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TRE-UBC-mPD-L1-T2A-GFP

   Plasmid

   #205455

   Purpose

   lentiviral plasmid for expression of mouse PD-L1

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   mPD-L1 (Cd274 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Flag-hPIASy

    Plasmid

    #15208

    Depositor

    Ke Shuai

    Article

    Liu et al Proc Natl Acad Sci U S A. 2001 Mar 13. 9

    Insert

    PIASy (PIAS4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-tetON-NKX2-1-EF1a-TagRFP-2A-tet3G

    Plasmid

    #167942

    Purpose

    Inducible NKX2-1 overexpression

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    TTF1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    tetON

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLY080b_AAV_EFS-4D5-CD8-28BBz (HER2 CAR)

    Plasmid

    #192190

    Purpose

    HER2 CAR AAV vector PRODH2 KI (pLY080b)

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    HER2 CAR AAV vector PRODH2 KI (pLY080b) (ERBB2 Synthetic)
    

    Use

    AAV; Mammalian expression

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-mCherry-CAAX

    Plasmid

    #129285

    Purpose

    Lentiviral plasmid for expression of fluorescent reporter mCherry targeted to the plasma membrane via CAAX polybasic sequence

    Depositor

    Carlos Carmona-Fontaine

    Article

    Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2

    Insert

    Fluorescent reporter mCherry fused to CAAX polybasic sequence
    

    Use

    Lentiviral

    Tags

    CAAX

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQAT05

    Plasmid

    #223377

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GFP-ERK2

    Plasmid

    #118296

    Purpose

    Expression of Rat ERK2

    Depositor

    Philip Stork

    Article

    Horgan et al Exp Cell Res. 2003 May 1;285(2):208-20.

    Insert

    ERK2 (Mapk1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJKR-H-benM

    Plasmid

    #73925

    Purpose

    Muconate biosensor. Expresses GFP proportional to muconate concentration within the cell.

    Depositor

    George Church

    Article

    Rogers et al Proc Natl Acad Sci U S A. 2016 Feb 8. pi

    Insert

    muconate biosensing apparatus
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBK-CMV Pig12

    Plasmid

    #16506

    Depositor

    Bert Vogelstein

    Article

    Polyak et al Nature. 1997 Sep 18. 389(6648):300-5.

    Insert

    Pig12 (PTGES Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pcdna3.1 hciap2mut

    Plasmid

    #8339

    Depositor

    Jon Ashwell

    Article

    Li et al Nature 2002 Mar 21;416(6878):345-7.

    Insert

    ciap2mut (BIRC3 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    H574A

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pOTTC352 - pAAV CaMKIIa eNpHR3.0-iRFP

    Plasmid

    #47904

    Purpose

    An AAV vector that expresses engineered halorhodopsin 3.0 (fused to iRFP) under the CaM Kinase IIa promoter.

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    eNpHR3.0
    

    Use

    AAV

    Tags

    iRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    Sept. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-N-WASP

    Plasmid

    #47406

    Depositor

    Peter McPherson

    Article

    Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

    Insert

    N-WASP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-EF1α-F-FLEX-mNaChBacMut-T2A-tdTomato

    Plasmid

    #60660

    Purpose

    Flp-dependent expression of mNaChBacMut-T2A-tdTomato

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Insert

    FLEX-mNaChBacMut-T2A-tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    E191K

    Promoter

    EF1α

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSEMS-Halo7Tag-hFis

    Plasmid

    #111136

    Purpose

    hFis with selflabeling tag, e.g. for fluorescence microscopy

    Depositor

    Karin Busch

    Article

    Appelhans et al Nano Lett. 2012 Feb 8;12(2):610-6. doi:

    Insert

    human fission factor1 (FIS1 Human)
    

    Use

    Tags

    Halo7-tag

    Expression

    Mammalian

    Mutation

    *

    Promoter

    CMV

    Available since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDONR221_SLC7A8

    Plasmid

    #132233

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC7A8 (SLC7A8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. phU1-PspCas13b-non-targeting (pEZ36)

    Plasmid

    #229053

    Purpose

    Cloning backbone for PspCas13b crRNA (Human U1 promoter)

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSLQ10704

    Plasmid

    #183957

    Purpose

    U6-SKSKSO(crRNA array)-EF1a-PuroR-T2A-BFP

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Insert

    SKSKSO array
    

    Use

    Lentiviral

    Tags

    EF1a-Puro-T2A-TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBAD-iPASnFR-L

    Plasmid

    #225238

    Purpose

    Bacterial expression of iPASnFR-L

    Depositor

    Loren Looger

    Article

    Tamura et al bioRxiv 2024.08.21.609037

    Insert

    iPASnFR-L
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Flag-hPIASx alpha

    Plasmid

    #15209

    Depositor

    Ke Shuai

    Article

    Arora et al J Biol Chem. 2003 Jun 13. 278(24):21327-

    Insert

    PIASx alpha (PIAS2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

28. pRM14

    Plasmid

    #163693

    Purpose

    hsp70 promoter inducible expression of DHB:mNeonGreen-P2A-H2B:mScarlet

    Depositor

    David Matus

    Article

    Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

    Insert

    DHB:mNeonGreen-P2A-H2B:mScarlet (HELB, H2BC11 Human)
    

    Use

    Unspecified; Zebrafish expression

    Tags

    mNeonGreen and mScarlet

    Expression

    Mutation

    Promoter

    HSP70

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCis mCherry-SS Himar1 A7

    Plasmid

    #84687

    Purpose

    Can be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.

    Depositor

    Ulrike Munderloh

    Article

    Munderloh et al Intracellular Pathogens II: Rickettsiale

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJRH-1187 VSVGmut

    Plasmid

    #201913

    Purpose

    Expresses a VSV-G variant (K47Q R354A) defective for LDL-R receptor binding (CAG promoter)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    VSV-G (K47Q, R354A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K47Q, R354A

    Promoter

    CAG

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge