Constitutive lentiviral expression of human APN.

Mouse sgRNA library in backbone lentiCRISPRv2 containing 78,637 unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls

Zebrafish ubiquitous specific expression of mKate2-P2A-TurboID-dVHH[GFP]. Parton lab clone JBH

Ready-to-use AAV Retrograde particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

Encodes GCaMP6s

Expression of hFerritinL in bacterial cells

Expression of HaloTag7 in mammalian cells

Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

episomal S*KM (expressing mouse super-Sox + Klf4 + Myc) for integration-free naïve reset

Express mEGFP-tagged fusion protein, FUS_FLI1 from patient-derived sequence

Lentivirus for inducible knockdown of human ARNTL(BMAL1)

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

High efficient, K+ selective channelrhodopsin for the inhibition of neurons

VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo

AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoter

Lentiviral expression of IRF9

For mammalian expression of human MEF2B with an HA tag

Can be used to subclone in gene of interest into blue motor neuron expression vector.

Doxycyline-inducible (TetOn) human Cdt1 (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)

human RNF160 (LTN1, ZNF294) FLAG mammalian expression vector.

Mammalian expression vector of flag-tagged RECQL5

Express C-terminally FLAG tagged gp78

Ready-to-use AAV PHP.eB particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA. CMV-driven EGFP control. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

GFP-tagged Myosin-19

a spaghetti monster with 10x HA epitopes repeat upstream of the HIV frameshift sequence which is followed by FLAG epitopes in the 0ORF are separated from one another by SunTag epitopes in the -1ORF.

AAV vector for coexpression of miniDq and KORD under the control of human synapsin promoter