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pCAG-H2B-GFP
Plasmid#184777PurposeExpresses H2B-GFP in human pluripotent stem cellsDepositorInsertH2BC11 (H2BC11 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
H2B-LSSmGFP
Plasmid#186539PurposeLocalization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to histone 2BDepositorInsertLSSmGFP
UseTagsHuman Histone 2B (D26G and V119I mutations)ExpressionMammalianMutationhfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206KPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRRlsinPGK_H2BGFP_WPRE
Plasmid#91788PurposeConstitutive expression of H2BGFP fusion using PGK promoter in a lentiviral backbone.DepositorInsertHuman histone H2B (H2BC11 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterPGKAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-H2B-6
Plasmid#56436PurposeLocalization: Nucleus/Histones, Excitation: 488, Emission: 507DepositorInsertH2B (H2BC11 Human)
UseTagsEGFPExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceDec. 23, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
sfGFP-H2B-6
Plasmid#56486PurposeLocalization: Nucleus/Histones, Excitation: 485, Emission: 507DepositorInsertH2B (H2BC11 Human)
UseTagssfGFPExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceJan. 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
sfGFP-H2B-C-10
Plasmid#56367PurposeLocalization: Nucleus/Histones, Excitation: 485, Emission: 507DepositorInsertH2B (H2BC11 Human)
UseTagssfGFPExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV.hSyn.flex.H2B.GFP
Plasmid#170368PurposeCre-inducible AAV vector expressing nuclear GFP.DepositorInsertflex H2B GFP
UseAAVTagsExpressionMutationPromoterhuman SynapsinAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
mPA-GFP-H2B-6
Plasmid#57137PurposeLocalization: Nucleus/Histones, Excitation: 400 / 504, Emission: 515 / 517DepositorInsertH2B (H2BC11 Human)
UseTagsmPA-GFPExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP‐RNASEH2B
Plasmid#108697PurposeFor expression of N-terminally eGFP-tagged human RNASEH2B in mammalian cellsDepositorInsertRNASEH2B (RNASEH2B Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
SOX2 KO (T2A-H2B-EGFP)
Plasmid#167969PurposeSOX2 CDS replacement with T2A-H2B-EGFPDepositorInsertSRY-Box Transcription Factor 2 (SOX2 Human)
UseCRISPR; Donor templateTagsH2B-EGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-52: HIST1H2BJ-mEGFP
Plasmid#109121PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human HIST1H2BJDepositorInsertHIST1H2BJ Homology Arms with linker-mEGFP (H2BC11 Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceMay 4, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin
Plasmid#99277PurposeDual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistanceDepositorInsertsH2B-mOrange2-IRES-EGFP
Puro
UseLentiviralTagsEGFP, H2B, IRES, PGK, and mOrange2ExpressionMutationPromoterEF1 alpha-human and PGK-mouseAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHIV-H2B-eGFP
Plasmid#91776PurposeExpresses H2B protein tagged with eGFPDepositorInsertHuman Histone H2B / eGFP (H2BC11 Human)
UseLentiviralTagseGFPExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS-H2B-EGFP
Plasmid#53744PurposeContains human Histone H2B fused to EGFP in the pCS2+ expression vectorDepositorInserthistone H2B (H2BC21 Human)
UseTagsEGFPExpressionMammalianMutationPromotersimian CMV IE94Available sinceJune 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFX-H2B-EGFP
Plasmid#175839PurposeExpress EGFP with nucleus-targeting sequence in mammalian cellsDepositorInsertEGFP with nucleus-targeting sequence
UseTagsH2B (human histone 2B)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
SOX9-T2A-H2B-EGFP repair template
Plasmid#167972PurposeSOX9 reporter repair templateDepositorInsertSRY-Box Transcription Factor 9 (SOX9 Human)
UseCRISPR; Donor templateTagsH2B-EGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Neo-CAG-M2rtTA-H2BGFP (donor AB)
Plasmid#85798PurposedonorAB targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP expression systemDepositorInsertPPP1R12C (PPP1R12C Human)
UseHuman targetingTagsExpressionMutationhomology arms for knock-in into first intronPromoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSIN-TRE-H2BeGFP-rtTA2
Plasmid#165494PurposeDoxycycline-inducible all-in-one lentivirus vector to express H2BeGFP fusion protein. System for labelling slow-cycling cells in vivo.DepositorInsertHuman histone H2B (H2BC21 Human)
UseLentiviralTagsEGFPExpressionMutation-PromoterTetO-CMVAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-loxP-H2B-sfGFP+pCI syntron-T2A-tdTomato+RbG and HsbG syntrons
Plasmid#172480PurposeH2B-sfGFP with a synthetic intron from the pCI expression vector, T2A, and tdTomato with synthetic introns derived from the rabbit beta-globin gene and the human beta-globin geneDepositorInsertH2B-sfGFP-T2A-tdTomato
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only
