168,165 results

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pSimple-miR302/367

Plasmid

#98748

Purpose

Episomal reprogramming

Depositor

Sara Howden, James Thomson

Article

Howden et al Stem Cell Reports. 2015 Dec 8;5(6):1109-

Insert

miR302/367 (miRNA cluster) (MIR302A, MIR367 Human)

Expression

Mammalian

Promoter

EF1alpha

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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TFORF2952

Plasmid

#144428

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TLE1 (TLE1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-DsRed FP (pMIDsRed II)

Plasmid

#52110

Purpose

a bicistronic IRES-DsRed containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

Depositor

Dario Vignali

Article

Holst et al Nat Protoc. 2006;1(1):406-17.

Type

Empty backbone

Use

Retroviral

Tags

IRES-DsRed

Expression

Mammalian

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pUAST-2x attB

Plasmid

#206362

Purpose

includes two standard UAS cassettes and a third cassette without a promoter

Depositor

Erdem Bangi

Article

Quintero et al Cell Rep. 2023 Nov 28;42(11):113370. doi

Type

Empty backbone

Expression

Insect

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pREDKI

Plasmid

#51626

Purpose

E coli Genetic Modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

I-SceI (SCEI Budding Yeast)

Use

Red recombinase and i-scei endonuclease

Expression

Bacterial

Promoter

pRha

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only

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pB-EF1a-FastFUCCI-IRES-3xnls-mTagBFP2 (JDW 1511)

Plasmid

#242599

Purpose

A PiggyBac expression vector containing the human EF1a promoter driving FastFUCCI to label cell cycle state followed by an IRES-3xnls-mTagBFP2.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110) (EEF1A1 Human)

Expression

Mammalian

Promoter

human EF1a

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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INS-CRa-Lsg-MS2

Plasmid

#247478

Purpose

sgRNA for INS activation cloned into LsgRNA-MS2 backbone

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Insert

sgRNA targeting INS promoter (INS Human, Synthetic)

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D-Ezrin T567D-BioID2-HA

Plasmid

#227682

Purpose

To over express Phospho-mimetic Ezrin under gfaABC1D promoter in astrocytes

Depositor

Cagla Eroglu

Article

Wang et al eLife 2025 [Reviewed Preprint] 14:RP1075

Insert

Ezrin (Ezr Mouse)

Tags

BioID2-HA

Expression

Mammalian

Mutation

T567D

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-CBh-mStayGold

Plasmid

#241379

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with CBh promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

CBh

Available Since

Sept. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_E_004_UAV_mScarlet-I

Plasmid

#235974

Purpose

Universal acceptor vector with mScarlet-I placeholder for lvl0 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

UAV_mScar

Use

Synthetic Biology

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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CROPseq-SFFV-eBFP2

Plasmid

#239601

Purpose

Expresses either functional CRISPR gRNAs from the hU6-promotor and empowers pairing of CRISPR screens with 3' and 5' scRNA-Seq capturing by FACS-sorting on eBFP2+ without prior resistance selection

Depositor

Christian Krebs

Article

Enk et al Sci Immunol. 2024 Jun 14;9(96):eadd6774.

Insert

spleen focus‑forming virus - promotor

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD S1406A

Plasmid

#169820

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported PKA phosphorylation site

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

S1406A; TCCC -> AGTC silent mutations at nt527…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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TH1203-pOCC177_pOEM1-N-HIS-MBP-PS-TAF15_opt-TEV-SNAP

Plasmid

#221891

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

TAF15 (TAF15 Human)

Tags

6xHis-MBP-PreScission and TEV-SNAP-tag

Expression

Insect

Mutation

wt

Promoter

PH promoter

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-RPA32

Plasmid

#211381

Purpose

mammalian expression of human RPA32 N-terminally fused to EGFP

Depositor

Helmut Pospiech

Insert

RPA32 (RPA2 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Histone H4-dimethyl-Arg3 [N309A/21R]

Plasmid

#206552

Purpose

Mammalian Expression Plasmid of anti-Histone H4-dimethyl-Arg3 (Human). Derived from hybridoma N309A/21.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Histone H4-dimethyl-Arg3 (Homo sapiens) recombinant Mouse monoclonal antibody (H4C14 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pJT166_GalL_A3A-BE3

Plasmid

#145116

Purpose

Expressing base editor A3A-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3A-BE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pET28b-6His-AcpS

Plasmid

#145376

Purpose

Expression of E. coli AcpS

Depositor

Robert Tomko

Article

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

AcpS (acpP E. coli)

Tags

6-His

Expression

Bacterial

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pKM468-EGFP

Plasmid

#108434

Purpose

ORBIT integrating plasmid for C-terminal tagging with EGFP.

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

attB-eGFP-4xGly-TEV-Flag-6xHis

Use

Orbit integrating plasmid

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ265E2

Plasmid

#164719

Purpose

Gateway entry clone (attL1 & attR5) A3A/Y130F-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

A3A(Y130F)-zCas9(D10A)-UGI

Use

CRISPR; Gateway compatible a3a/y130f-cbe_v01 entr…

Expression

Plant

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pFast-hRad1

Plasmid

#222471

Purpose

Expresses hRad1 in insect cells

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

Insert

hRad1 (RAD1 Human)

Expression

Insect

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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p5154

Plasmid

#175390

Purpose

High Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts <15kb) into dual site mouse strains (Bxb-GT/GA)

Depositor

Michael Wiles

Article

Low et al Sci Rep. 2022 Mar 31;12(1):5424. doi: 10

Type

Empty backbone

Use

Mouse Targeting; Cloning vector, to be used to de…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCT5-bac2.0

Plasmid

#119872

Purpose

Cumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coli

Depositor

Claudia Schmidt-dannert

Article

Seo et al Appl Microbiol Biotechnol. 2018 Nov 3. p

Inserts

CymR
sfGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pveg promoter with CuO sequence and PxylR promoter

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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TFORF2540

Plasmid

#142321

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SOX5 (SOX5 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_eCB2.0-IRES-mCherry-CAAX

Plasmid

#164611

Purpose

Express the endocannabinoid sensor GRAB_eCB2.0 in mammalian cells

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid sensor GRAB_eCB2.0

Expression

Mammalian

Mutation

S383T

Promoter

CMV

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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LentiGuide-puro-NLS-GFP

Plasmid

#185473

Purpose

Mammalian Expression, Lentiviral vector expressing GFP for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Type

Empty backbone

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pLX-sgRNA

Plasmid

#50662

Purpose

Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)

Depositor

Eric Lander, David Sabatini

Article

Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

Insert

sgAAVS1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pET3a-OSF-CypA

Plasmid

#79039

Purpose

Bacterial vector for cloning and propagation in DH5alpha

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OneSTrEP-FLAG- Cyclophilin A (PPIA Human)

Tags

OneSTrEP-FLAG

Expression

Bacterial

Promoter

T7

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.B

Plasmid

#103002

Purpose

non-standard AAV2 rep-AAV-PHP.B cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

Insert

Synthetic construct isolate AAV-PHP.B VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-E1799K

Plasmid

#69009

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-E1799K (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Glutamate 1799 to Lysine

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-beta-catenin deltaN90

Plasmid

#36985

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

beta-catenin deltaN90 (CTNNB1 Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

Deletion of amino acids 1-90 (Constitutively Acti…

Promoter

EF1a

Available Since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only

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