168,189 results

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RGG-BcLOV-mCh

Plasmid

#208276

Purpose

Expresses RGG-BcLOV fusion for optogenetic membrane recruitment. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

RGG-BcLOV-mCh

Expression

Mammalian

Promoter

pCMV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pEM705 Gst (Ser)

Plasmid

#109354

Purpose

GsX chimera with Gαt C-terminus (Pertussis toxin insensitive mutant)

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gst chimera

Tags

None

Expression

Mammalian

Mutation

Changed Cysteine at position 391 to Serine (Pertu…

Promoter

CAG

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-RLuc8.6-535

Plasmid

#87125

Purpose

Mammalian expression vector expressing red shifted variant of the reporter gene-Renilla luciferase (Rluc8.6)

Depositor

Sanjiv Sam Gambhir

Article

Loening et al Nat Methods. 2007 Aug;4(8):641-3. Epub 2

Insert

Renilla Luciferase

Expression

Mammalian

Mutation

RLuc8/ A123S/D154M/E155G/D162E/I163L/V185L.

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pET21b Apaf1 1-591

Plasmid

#26642

Depositor

Guy Salvesen

Article

Pop et al Mol Cell. 2006 Apr 21. 22(2):269-75.

Insert

Apoptotic protease activating factor 1 (APAF1 Human)

Tags

His

Expression

Bacterial

Mutation

Amino Acids 1-591

Available Since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only
CMV-mCherry-3’UTR of β-actin-(F30-2xdBroccoli)6

Plasmid

#190162

Purpose

Expresses 24 Broccoli-tagged beta-actin mRNA in mammalian cells

Depositor

Samie Jaffrey

Article

Li et al Angew Chem Int Ed Engl. 2020 Mar 9;59(11

Insert

mcherry_(3'UTR, No Actin)_24Broccoli

Tags

24×Broccoli

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pEBTetD-SNAP-Centrin2

Plasmid

#136785

Purpose

Mammalian expression of the centrosomal protein Centrin2 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Centrin2 (CETN2 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX GFP-HA

Plasmid

#229501

Purpose

Control HA-tagged protein for immunoprecipitation experiments.

Depositor

Davide Ruggero

Article

Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

Insert

eGFP

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-hSncg-EGFP

Plasmid

#153198

Purpose

Human gamma-synuclein (hSncg) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBID-UASC

Plasmid

#35200

Depositor

Brian McCabe

Article

Wang et al PLoS One. 2012;7(7):e42102. Epub 2012 Ju

Type

Empty backbone

Use

Drosophila transgene expression

Promoter

Drosophila Synthetic Core Promoter (DSCP)

Available Since

July 26, 2012

Availability

Academic Institutions and Nonprofits only

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pC0056 - LwCas13a-msfGFP-NES

Plasmid

#105815

Purpose

Expresses active LwaCas13a-NES for mammalian RNA targeting

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LwCas13a

Use

CRISPR and Lentiviral

Tags

NES and msfGFP-NES-3xHA

Expression

Mammalian

Promoter

EF-1a

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pMC-6-BeYDV-Rep-RepA-9

Plasmid

#197715

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

BeYDV Rep/RepA proteins: native ORFs

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-HsSAS6

Plasmid

#136789

Purpose

Mammalian expression of the centrosomal protein SAS6 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-SAS6 (SASS6 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Centrin2

Plasmid

#136784

Purpose

Mammalian expression of the centrosomal protein Centrin2 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Centrin2 (CETN2 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pDA083

Plasmid

#131389

Purpose

eGFP expression

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

eGFP

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG wildtype, catalytic active lipin 1

Plasmid

#32005

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

lipin1 (Lpin1 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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TFORF2718

Plasmid

#142131

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

LRPPRC (LRPPRC Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) mouse C/EBP beta (LAP)

Plasmid

#12557

Depositor

Peter Johnson

Insert

CCAAT/Enhancer-binding Protein beta (Cebpb Mouse)

Expression

Mammalian

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pAF0688 Kp03_attP-mCherry donor

Plasmid

#193467

Purpose

Promoterless mcherry donor for Kp03

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Kp03_attP

Expression

Mammalian

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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RNF43-SmBiT, BiBiT-Ready Vector

Plasmid

#237081

Purpose

Express RNF43-SmBiT Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

RNF43 (RNF43 Human)

Tags

SmBiT

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pBLind

Plasmid

#113109

Purpose

Vector encoding the RFP gene under blue light-inducible promoter BindF1.

Depositor

Chueh Loo Poh

Article

Jayaraman et al ACS Synth Biol. 2018 Apr 20;7(4):986-994

Insert

RFP

Use

Cell-free

Expression

Bacterial

Promoter

BindF1

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pDR196

Plasmid

#36029

Depositor

Wolf Frommer, Dominique Loque, Doris Rentsch

Article

Meyer et al J Biol Chem. 2006 Mar 17;281(11):7197-20

Type

Empty backbone

Expression

Yeast

Available Since

June 21, 2012

Availability

Academic Institutions and Nonprofits only

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pZR158_Lenti-U6-gRNA-10xCS1-PP7_hPGK-PCP-P65-HSF1-Puro

Plasmid

#180273

Purpose

Lentiviral expression vector for CRISPRa-SAM sgRNA with 10x capture sequence, and PCP-P65-HSF1 cassette

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pSEVA3b61

Plasmid

#58310

Purpose

Shuttle vector: pUC ori, pTHT15 ori for use in E. coli/B. subtilis. Cannot modify orientation of cat gene; promoter too weak in E. coli, probably transcribed via repB promoter in bicistronic manner.

Depositor

Tom Ellis

Article

Wright et al ACS Synth Biol. 2014 May 13.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only

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LVXN-Neo-NSD2-E1099K

Plasmid

#86011

Purpose

express NSD2 E1099K mutant in mammalian cells

Depositor

Darrin Stuart

Article

Jaffe et al Nat Genet. 2013 Nov;45(11):1386-91. doi:

Insert

NSD2 E1099K mutant (NSD2 Human)

Tags

Flag tag D Y K D D D D K

Expression

Mammalian

Mutation

E1099K; K1002R (please see depositor comments bel…

Promoter

CMV

Available Since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only
pRRLSIN.cPPT.PGK.NeuroD2.WPRE

Plasmid

#122054

Purpose

Lentiviral expression of NeuroD2

Depositor

Stephen Tapscott

Article

Fong et al Cell Rep. 2015 Mar 31;10(12):1937-46. do

Insert

NeuroD2 (Neurod2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-EGFP-NLS-FLAG-cGAS

Plasmid

#127659

Purpose

Over-express EGFP-NLS-FLAG-cGAS

Depositor

Nicolas Manel

Article

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

cGAS (CGAS Human)

Use

Lentiviral

Tags

EGFP-NLS-Flag

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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VinculinTS-A50I/I997A

Plasmid

#111829

Purpose

Vinculin tension sensor with point mutations affecting ability to bind talin and actin

Depositor

Brenton Hoffman

Article

Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

Insert

VinculinTS (I997A)

Expression

Mammalian

Mutation

changed vinculin alanine 50 to isoleucine and iso…

Promoter

CMV

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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GST-p35

Plasmid

#24895

Depositor

Harish Pant

Article

Amin et al J Neurosci Res. 2002 Feb 1. 67(3):354-62

Insert

p35 (CDK5R1 Human)

Tags

GST

Expression

Bacterial

Mutation

Full-length p35

Available Since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(-)-EGFP-FP4-mito

Plasmid

#229692

Purpose

Transient expression of EGFP FP4-mito in mammalian cells

Depositor

Alpha Yap

Article

Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

Insert

EGFP; Mitochondira FP4 motifs from Listeria ActA

Tags

EGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV: Flag-Antares_SV40:PuroR

Plasmid

#183049

Purpose

Expresses FLAG-tagged Antares in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

Antares BRET reporter

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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