159,258 results

1. gRNA-CasRx_SD1-pSV40-TagBFP

   Plasmid

   #221004

   Purpose

   Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.

   Depositor

   Benjamin Blencowe, Mikko Taipale

   Article

   Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

   Insert

   CasRx 30nt processed direct repeat with SD1 mutation
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   DR1 A>T

   Promoter

   U6

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pON.mCherry

   Plasmid

   #84821

   Purpose

   Constitutive Expression plasmid with mCherry fluorescent protein

   Depositor

   Howard Shuman

   Article

   Gebhardt et al PLoS One. 2017 Mar 3;12(3):e0173116. doi

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tac Promoter

   Available since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO GFP HSPH1

   Plasmid

   #19480

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

   Insert

   HSPH1 (HSPH1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO V5 HSPH1

   Plasmid

   #19506

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

   Insert

   heat shock 105kDa/110kDa protein 1 (HSPH1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSIN-TRE-H2BeGFP-rtTA2

   Plasmid

   #165494

   Purpose

   Doxycycline-inducible all-in-one lentivirus vector to express H2BeGFP fusion protein. System for labelling slow-cycling cells in vivo.

   Depositor

   Hector G. Palmer

   Article

   Puig et al J Clin Invest. 2018 Aug 31;128(9):3887-3

   Insert

   Human histone H2B (H2BC21 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   -

   Promoter

   TetO-CMV

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDH-EF1-Luc2-P2A-tdTomato

   Plasmid

   #72486

   Purpose

   Express Luc2 and tdTomato under EF1 promoter

   Depositor

   Kazuhiro Oka

   Insert

   Luc2-P2A-tdTomato
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Jan. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-Puro-TDP-43-WT

   Plasmid

   #133753

   Purpose

   Wild type human TDP-43 in pLVX-Puro lentiviral vector

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. cEF.tk-GFP

   Plasmid

   #33308

   Depositor

   Linzhao Cheng

   Article

   Pomper et al Cell Res. 2009 Mar;19(3):370-9.

   Insert

   HSV-TK/GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETDuet1_6xHis-TEV-Lambda-Protein-Phosphatase

   Plasmid

   #223747

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   Lambda-PPase
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. FUW-Luc-mCherry-puro

    Plasmid

    #183685

    Purpose

    Lentiviral vector expressing luciferase and mCherry.

    Depositor

    Andrew Kung

    Article

    Kimbrel et al Mol Imaging. 2009 May-Jun;8(3):140-7.

    Insert

    Luc-E2A-mCherry-T2A-PuroR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Human Ubiquitin C (UbC) promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDH_Str-KDEL_ManII-SBP-mCherry

    Plasmid

    #65259

    Purpose

    synchronize trafficking of ManII from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin-KDEL and truncated Mannosidase II
    

    Use

    Lentiviral

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV_hSyn1-SIO-stGtACR2-FusionRed

    Plasmid

    #105677

    Purpose

    Expresses a soma-targeted GtACR2 in Cre-expressing neurons

    Depositor

    Ofer Yizhar

    Article

    Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

    Has Service

    AAV Retrograde, AAV1, and AAV5

    Insert

    stGtACR2
    

    Use

    AAV

    Tags

    FusionRed and Kv2.1

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn1

    Available since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. IRE1 alpha-pcDNA3.EGFP

    Plasmid

    #13009

    Depositor

    Fumihiko Urano

    Article

    Lipson et al Cell Metab. 2006 Sep . 4(3):245-54.

    Insert

    Inositol Requiring Enzyme-1 (ERN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-Mito4x-jRCaMP1b

    Plasmid

    #127873

    Purpose

    Red-shifted fluorescent reporter for mitochondrial matrix Ca2+

    Depositor

    Timothy Ryan

    Article

    Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

    Insert

    Red-shifted mitochondrial matrix Ca2+ indicator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCIP-Flag-TfebAA

    Plasmid

    #79014

    Purpose

    For mammalian expression of a Flag-tagged, constitutively active version (S142A, S211A) of Tfeb.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    Tfeb (Tfeb Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S142A, S211A

    Promoter

    pCAGGs

    Available since

    July 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDUAL CD81 (GFP)

    Plasmid

    #86980

    Purpose

    Lentiviral expression construct encoding CD81 and GFP from separate promoters

    Depositor

    Joe Grove

    Insert

    CD81 (CD81 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV/hPGK

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. sgRNA_expression_vector

    Plasmid

    #210212

    Purpose

    An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.

    Depositor

    Albert Jeltsch

    Article

    Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. P2X4-pHluorin123

    Plasmid

    #52926

    Purpose

    express P2X4 protein with pHluorin at 123rd position

    Depositor

    Baljit Khakh

    Article

    Xu et al J Gen Physiol. 2014 Jul;144(1):81-104. d

    Insert

    mP2X4-pHluorin123
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 23, 2014

    Availability

    Academic Institutions and Nonprofits only

19. 3xFLAG-NanoLuc

    Plasmid

    #189975

    Purpose

    Part 3-4-5 plasmid to introduce 3xFLAG-NanoLuc into circRNAs

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    NanoLuc
    

    Use

    Cloning

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    N/A

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT3-EF1a-NICD1

    Plasmid

    #46047

    Depositor

    Xin Chen

    Article

    Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

    Insert

    Notch1 (C-term) (Notch1 Mouse)
    

    Use

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Cytoplasmic domain initiating at amino acid 1753

    Promoter

    EF1a

    Available since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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21. pETCON-HtsptLB12v.3

    Plasmid

    #45121

    Depositor

    David Baker

    Article

    Procko et al J Mol Biol. 2013 Sep 23;425(18):3563-75.

    Insert

    HtsptLB12v.3
    

    Use

    Tags

    Aga2p for yeast surface display and c-myc epitope…

    Expression

    Yeast

    Mutation

    Promoter

    GAL

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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22. SPLICS LY-MT Long P2A

    Plasmid

    #213613

    Purpose

    Detect the long-range Lysosome-Mitochondria contact

    Depositor

    Tito Calì

    Article

    Giamogante et al Nat Commun. 2024 Feb 19;15(1):1516. doi:

    Insert

    SPLICS LY-MT Long P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1-P2X2-YFP

    Plasmid

    #22400

    Depositor

    Henry Lester

    Article

    Khakh et al J Neurosci. 2005 Jul 20. 25(29):6911-20.

    Insert

    ATP-gated P2X channel 2 (P2rx2 Rat)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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24. pSpCas9n(BB)-2A-Puro (PX462) V2.0

    Plasmid

    #62987

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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25. pT7-7 asyn WT

    Plasmid

    #36046

    Depositor

    Hilal Lashuel

    Article

    Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

    Insert

    alpha-synuclein (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-RSV-SpCas9

    Plasmid

    #85450

    Purpose

    Express SpCas9 in mammalian cells

    Depositor

    Hetian Lei

    Article

    Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

    Insert

    pRSV
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    pRSV

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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27. pLVX-Puro-TDP-43-A315T

    Plasmid

    #133755

    Purpose

    Lentiviral expression of human TDP-43 A315T disease mutant in mammalian cells.

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed alanine 315 to threonine

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mRNA-CleanCap-mNeonGreen

    Plasmid

    #188248

    Purpose

    Template plasmid to transcribe mRNA encoding mNeonGreen

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV hSyn GFP-Cre

    Plasmid

    #175381

    Purpose

    expresses GFP-Cre under the control of a human synapsin promoter

    Depositor

    Jason Aoto

    Insert

    GFP-Cre recombinase
    

    Use

    AAV

    Tags

    Cre is fused to GFP. GFP is fused to additional n…

    Expression

    Mutation

    Promoter

    human synapsin

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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30. pC.HA Vav2

    Plasmid

    #14554

    Depositor

    Joan Brugge

    Article

    Moores et al Mol Cell Biol. 2000 Sep . 20(17):6364-73

    Insert

    Vav2 (VAV2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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