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27,466 results

Showing: 4701 - 4720 of 27466 results

  1. pDAC435

    Plasmid
    #195238
    Purpose
    Mammalian expression of Csm complex from separate promoters; RFP backbone (Used for RNA knockdown)
    Depositor
    Jennifer Doudna
    Publication
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FLAG-NLS-Csm1;FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; RFP
    Expression
    Mammalian
    Available since
    Jan. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    195238-plasmid-map-sequence-id-382946

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  3. pcDNA3.1-mhYFP

    Plasmid
    #186533
    Purpose
    Monomeric hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cells
    Depositor
    Gregory Petsko
    Publication
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Monomeric hyperfolder YFP
    Expression
    Mammalian
    Mutation
    hfYFP-S147P/L195M/V206K
    Promoter
    CMV
    Available since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186533-plasmid-map-sequence-id-368148

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  5. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

    Plasmid
    #183411
    Purpose
    piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.
    Depositor
    Azim Surani
    Publication
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tet-on 3G
    Use
    CRISPR and Synthetic Biology; Piggybac
    Expression
    Mammalian
    Promoter
    EF1A
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    183411-plasmid-map-sequence-id-361257

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  8. AttB_ACE2_IRES-mCherry-H2A-P2A-PuroR

    Plasmid
    #171594
    Purpose
    Strong human ACE2 abundance from Bxb1 landing pad
    Depositor
    Kenneth Matreyek
    Publication
    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ACE2 (ACE2 Human)
    mCherry
    H2A
    Pac
    Use
    Synthetic Biology; Promoterless bxb1 dna recombin…
    Expression
    Mammalian
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    171594-plasmid-map-sequence-id-335073

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  11. pC1-CMV-mito-DAAO

    Plasmid
    #141132
    Purpose
    Expresses DAAO with mitochondrial localization signal in mammalian cells
    Depositor
    Vsevolod Belousov
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mito-DAAO
    Tags
    2 mitochondrial localization signals and 7xHis
    Expression
    Mammalian
    Mutation
    deleted DAAO amino acids 367-368
    Promoter
    CMV
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    141132-plasmid-map-sequence-id-323598

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  16. pcDNA5/FRT/TO-GAlphaQ-RLuc8

    Plasmid
    #140982
    Purpose
    Encodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAlphaQ-RLuc8 (GNAQ Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140982-plasmid-map-sequence-id-300744

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  17. pcDNA3-sACE2(WT)

    Plasmid
    #145147
    Purpose
    Mammalian expression plasmid for soluble ACE2 (protease domain)
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    145147-plasmid-map-sequence-id-292583

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Showing: 4701 - 4720 of 27466 results