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pCAGGS-4493NES

Plasmid

#138373

Purpose

Booster-PKA, a Red-Shifted Genetically Encoded FRET Biosensor for monitoring PKA activity.

Depositor

Michiyuki Matsuda

Publication

Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Booster-PKA

Expression

Mammalian

Promoter

CAG

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pEF-AncBE4max

Plasmid

#138270

Purpose

Expression of AncBE4max-Cas9 Base-editor from an EF1a promoter.

Depositor

Xiao Wang

Publication

Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AncBE4max

Use

CRISPR

Expression

Mammalian

Mutation

D10A

Promoter

Ef1a

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCMV-Cas9-NRCH

Plasmid

#136926

Purpose

Expresses Cas9-NRCH in mammalian cells

Depositor

David Liu

Publication

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9-NRCH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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mNeongreen-PXN

Plasmid

#129604

Purpose

In vivo visualization of focal adhesions (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Paxillin

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pME-Kaede

Plasmid

#132972

Purpose

full-length of photoconvertible kaede with stop codon in pmE Gateway vector

Depositor

Nathan Lawson

Publication

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Kaede

Use

Gateway vector

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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TGN38 EGFP

Plasmid

#128148

Purpose

Expression of TGN38 amino acids 304-357

Depositor

Jennifer Lippincott-Schwartz

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TGN38 (Tgoln2 Rat)

Tags

mEGFP

Expression

Mammalian

Mutation

Contains amino acids 304-357

Available since

Nov. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCD86-EGFP

Plasmid

#133858

Purpose

Mammalian expression of CD86 C-terminally tagged with EGFP

Depositor

Dave Piston

Publication

Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CD86 (CD86 Human)

Tags

EGFP

Expression

Mammalian

Available since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCIneoGFP-BMI1

Plasmid

#128328

Purpose

Mammalian expression vector of GFP-tagged human BMI1

Depositor

Yutaka Hata

Publication

Hossain et al Mol Cell Biol. 2018 Aug 15;38(17). pii:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BMI1 (BMI1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1+/-FLI1-C‐(K)‐DYK

Plasmid

#129497

Purpose

Mammalian expression of human FLI1

Depositor

Marjorie Brand

Publication

Palii et al Cell Stem Cell. 2019 May 2;24(5):812-820

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FLI1 (FLI1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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HA-TRIM28deltaPB

Plasmid

#124959

Purpose

Mammalian expression of Human TRIM28 with deleted PHD and BROMO domain

Depositor

Michelle Barton

Publication

Li et al Oncogene. 2017 May 25;36(21):2991-3001.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hTRIM28 (TRIM28 Human)

Tags

HA

Expression

Mammalian

Mutation

Deletion of PHD and Bromo Domain

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBT120b

Plasmid

#122604

Purpose

BE PACE selection, express gIII, luxAB and degron-GTCCA guide RNA

Depositor

David Liu

Publication

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gIII, luxAB, guide RNA

Use

Synthetic Biology

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBT376

Plasmid

#125617

Purpose

Expresses BE4max-NG in mammalian cells

Depositor

David Liu

Publication

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BE4max-NG

Expression

Mammalian

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Influenza-FPV-HA-mEGFP

Plasmid

#127810

Purpose

Expression vector Influenza A virus FPV (H7N1) hemagglutinin mEGFP fusion protein, plasma membrane localization

Depositor

Salvatore Chiantia

Publication

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Influenza A virus FPV (H7N1) hemagglutinin mEGFP fusion

Expression

Mammalian

Available since

June 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMF1762

Plasmid

#125583

Purpose

Expresses mitochondrial roGFP2 (mt-roGFP2) in mammalian cells

Depositor

Marc Fransen

Publication

Ivashchenko et al Mol Biol Cell. 2011 May;22(9):1440-51. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mt-roGFP2

Expression

Mammalian

Promoter

CMV

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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PB-TetON-dual-SunTag-Hygro

Plasmid

#121119

Purpose

Endogenous gene activation with the SunTag CRISPR activation system

Depositor

Pablo Navarro

Publication

Heurtier et al Nat Commun. 2019 Mar 7;10(1):1109. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9-GCN4x10_scFv-VP64

Use

CRISPR

Expression

Mammalian

Promoter

Tet-ON

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ArchT-BFP2-TSERex

Plasmid

#123312

Purpose

Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

Depositor

Yulong Li

Publication

Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ArchT-BFP2

Expression

Mammalian

Promoter

CAG

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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cycLuc_TEVS

Plasmid

#119207

Purpose

Circularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)

Depositor

Roman Jerala

Publication

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

cyclic luciferase with TEVs cleavage site

Use

Luciferase

Tags

6xHis and AU1 tag

Expression

Mammalian

Promoter

CMV

Available since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pSpCas9(BB)-T2A-HygR

Plasmid

#118153

Purpose

SpCas9 expression vector

Depositor

Jorge Ferrer

Publication

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hSpCas9-T2A-HygR

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Promoter

CAG and U6

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX330-sgAAVS1

Plasmid

#85802

Purpose

Cas9/CRISPR vector for cut at human AAVS1 gene intron1

Depositor

Roland Friedel

Publication

Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PPP1R12C (PPP1R12C Human)

Expression

Mammalian

Available since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMK365 (Tet-OsTIR1-loxP-PURO-loxP)

Plasmid

#121185

Purpose

OsTIR1 introduction

Depositor

Masato Kanemaki

Publication

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Tet-OsTIR1-loxP-PURO-loxP

Use

CRISPR

Mutation

OsTIR1 codon optimized for human cells

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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