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27,466 results

Showing: 10821 - 10840 of 27466 results

  7. pcDNA3.1-Hygro(+)-PSTCD-cGAS-delta DBD

    Plasmid
    #102606
    Purpose
    Mammalian expression of mutant cGAS with deletion of DNA binding domain fused to PSTCD tag
    Depositor
    Nicolas Manel
    Publication
    Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cGAS (CGAS Human)
    Expression
    Mammalian
    Mutation
    delta K173-I220 and delta H390-C405
    Promoter
    CMV
    Available since
    Jan. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    102606-plasmid-map-sequence-id-202129

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  8. pCAG-EnvARGCD

    Plasmid
    #100800
    Purpose
    express fusion protein of EnvA (ASLV-A envelope protein) extracellular and transmembrane domains with rabies virus SAD B19 strain glycoprotein intracellular domain
    Depositor
    Ian Wickersham
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    fusion protein of EnvA extracellular and transmembrane domain with rabies virus glycoprotein intracellular domain
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Dec. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    100800-plasmid-map-sequence-id-189516

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  12. pEGFP-NMHC-IIA-S1916A

    Plasmid
    #101040
    Purpose
    Expresses GFP-MYH9 construct (human myosin IIA) carrying mutation of serine 1916 to alanine, driven by CMV promoter. Thus inhibits PKC phosphorylation of the tail. Also carries S1915A mutation.
    Depositor
    Tom Egelhoff
    Publication
    Rai et al J Biol Chem. 2017 Feb 24;292(8):3099-311
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Non-muscle myosin IIA heavy chain (MYH9 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    S1916A, S1917A
    Promoter
    CMV
    Available since
    Nov. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101040-plasmid-map-sequence-id-198956

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  14. [Bla][IRES][GFP]

    Plasmid
    #97193
    Purpose
    Vector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.
    Depositor
    Edward Boyden
    Publication
    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    beta-Lactamase
    GFP
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    97193-plasmid-map-sequence-id-197876

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  15. pX330-Flag-deSpCas9

    Plasmid
    #92114
    Purpose
    Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead/inactive eSpCas9 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A, K848A, K1003A, R1060A
    Promoter
    Cbh
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92114-plasmid-map-sequence-id-197615

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  18. p330 VQR

    Plasmid
    #101730
    Purpose
    Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs
    Depositor
    Andrew Holland
    Publication
    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 VQR
    Tags
    3xFLAG-NLS and NLS
    Expression
    Mammalian
    Mutation
    VQR (D1135V, R1335Q, and T1337R mutations in Cas9)
    Available since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101730-plasmid-map-sequence-id-192072

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  19. BPK3258 - human expression plasmid for eSpCas9(1.1)

    Plasmid
    #101176
    Purpose
    Human expression plasmid for SpCas9 eSpCas9(1.1) variant: CMV-T7-hSpCas9-eSpCas9(1.1)(K848A, K1003A, R1060A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9-eSpCas9(1.1)(K848A/K1003A/R1060A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    K848A/K1003A/R1060A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101176-plasmid-map-sequence-id-193999

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  20. pCMV sMTase (eGFP)

    Plasmid
    #89930
    Purpose
    Expresses sMTase system for targeted DNA methylation in mammalian cells. Contains an eGFP marker expressed off separate promoter.
    Depositor
    Carl Novina
    Publication
    Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-MC-IRES-MN
    Tags
    Flag and HA
    Expression
    Mammalian
    Mutation
    deactivated Cas9
    Promoter
    CMV
    Available since
    Sept. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    89930-plasmid-map-sequence-id-178000

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Showing: 10821 - 10840 of 27466 results