27,007 results
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Plasmid#126463PurposeExpression a human MOG (isoform alpha1) EGFP fusion protein in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMyelin oligodendrocyte glycoprotein (MOG Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV_ABEmax_3xHA
Plasmid#112098PurposeA:T-to-G:C base editingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertABEmax_3xHA
UseCRISPRTags3xHAExpressionMammalianPromoterCMVAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL4-AARE-luc2P-Hygro
Plasmid#101787PurposeLuferase reporter plasmid containing three tandem repeats of the amino acid response element (AARE)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3xAARE
UseLuciferaseTagsLuciferaseExpressionMammalianPromoterminimal TATA-box promoter with low basal activityAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AICSDP-13:FBL-mEGFP
Plasmid#87427PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human FBLDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFBL Homology Arms with linker-mEGFP (FBL Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available sinceMarch 15, 2017AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pCX-DI-2A
Plasmid#74671PurposeMammalian expression of DI-2A (hCD73/F2A/hCD39)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNATagsF2AExpressionMammalianPromoterpCAGGSAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Rab21
Plasmid#83421PurposeMouse Rab21 (wild type)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRab21 (Rab21 Mouse)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV10-3xFlag Skp2 WT
Plasmid#81115Purposemammalian expression of Skp2 WTDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSKP2 (SKP2 Human)
Tags3xFlagExpressionMammalianPromoterCMVAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUB_smHA_KDM5B_MS2
Plasmid#81085PurposeTranslation reporter: Encodes KDM5B (1544 aa) fused to smHA (Spaghetti Monster HA: 10 copies of an epitope tag HA).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsmHA_KDM5B_24xMS2
ExpressionMammalianAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTriEx-mCherry-LOV2 V416L
Plasmid#81036Purposemammalian expression of LOV V416L, a mutant with super long relaxation timeDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLOV V416L
TagsmCherryExpressionBacterial, Insect, and Mamm…MutationV416LAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKHR7
Plasmid#74624Purposea cmlc2::mCherry (red heart) reporter gene was inserted at the 3’end of pKHR4 mcs, that resides within donor fragments produced by I-SceI digestion. Genbank accession number is KU144824.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcmlc2::mCherry
UseZebrafish expressionPromotercmlc2Available sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKanCMV-mClover3-18aa-Tubulin
Plasmid#74253PurposeMammalian expression of N-terminal fusion of mClover3 to TubulinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTubulin
TagsmClover3ExpressionMammalianPromoterCAG and CMVAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-OptoSTIM1(Cry2(D387A))
Plasmid#70160PurposeLight-insensitive mutant of OptoSTIM1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOptoSTIM1(Cry2(D387A)) (STIM1 Mustard Weed, Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEF_dCas9
Plasmid#68416PurposeTransient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9
UseCRISPRTags3xFLAGExpressionMammalianMutationD10A/H841APromoterhuman EF1[alpha]Available sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GCGR-Tango
Plasmid#66291PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGCGR (GCGR Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Beta-2-adrenergic receptor-YFP
Plasmid#67146PurposeBeta-2-adrenergic receptor tagged with YFP on carboxyl terminusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBeta-2-adrenergic receptor-YFP (ADRB2 Aequorea victoria, Human)
TagsYFPExpressionMammalianPromoterCMVAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
G protein alpha-q-GFP
Plasmid#66080PurposeThis G protein alpha-q construct contains internal insertions of GFP and the EE epitopeDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertG protein alpha-q-EE-YFP (Gnaq Mouse)
TagsGFP was inserted internally into the alpha-q sequ…ExpressionMammalianMutationBamHI and SacI sites in alpha-q were removed with…PromoterCMVAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-TORCAR-NLS
Plasmid#64931PurposeNuclear-targeted FRET biosensor for monitoring mTORC1 kinase activity.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTORCAR-NLS (EIF4EBP1 Synthetic, Human)
TagsCerulean, Nuclear localization signal (NLS), and …ExpressionMammalianPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-ATAD2
Plasmid#65370PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertATAD2 (ATAD2 Human)
TagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAMPK alpha2 delta312X
Plasmid#60127Purposeexpresses constitutively active AMPKalpha2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAMPK alpha 2 (Prkaa2 Rat)
TagsMycExpressionMammalianMutationTruncated at residue 312Available sinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-Vinculin-N-21
Plasmid#55160PurposeLocalization: Focal Adhesions, Excitation: 587, Emission: 610DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVinculin (VCL Human)
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by