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27,006 results

Showing: 5141 - 5160 of 27006 results
  1. pcDNA5/FRT/TO-GAlphasS-RLuc8

    Plasmid
    #140980
    Purpose
    Encodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAlphasS-RLuc8
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  2. dead Sa Cas9

    Plasmid
    #138162
    Purpose
    For orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dSaCas9
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    D10A + N580A
    Promoter
    CMV
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  3. pEBTet_NAD-Snifit-mito

    Plasmid
    #113916
    Purpose
    Plasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NAD-Snifit
    Expression
    Mammalian
    Promoter
    CMV TetO2
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  4. pcDNA 3.1 SIRT1-FL

    Plasmid
    #105670
    Purpose
    Mammalian expression construct of mouse SIRT1 full length isoform
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SIRT1 (Sirt1 Mouse)
    Expression
    Mammalian
    Mutation
    Synonymous base changes not affecting the protein…
    Promoter
    CMV
    Available since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  5. xCas9(3.6)-BE3

    Plasmid
    #108385
    Purpose
    Mammalian expression vector for xCas9(3.6)-BE3
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    xCas9(3.6)-BE3
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …
    Available since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
  6. VPR-ps-dSpCas9

    Plasmid
    #102855
    Purpose
    A single-chain light-controllable dSpCas9 with pdDronpa1.2 domains
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dSpCas9, pdDronpa1.2
    Use
    CRISPR and Synthetic Biology
    Tags
    NLS and VP64-p64-Rta transcription activator
    Expression
    Mammalian
    Promoter
    PGK
    Available since
    Nov. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
  7. pMOD_B2303

    Plasmid
    #91068
    Purpose
    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    Use
    CRISPR
    Promoter
    CmYLCV
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  8. pCMV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

    Plasmid
    #92390
    Purpose
    expresses Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA component in mammalian cells
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  9. MCS+

    Plasmid
    #90412
    Purpose
    A FRET sensor that report the electric potential at the inner leaflet of cell plasma membrane
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lck10+ Venus+mcherry+ strech of positively charged amino acids
    Expression
    Mammalian
    Available since
    May 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 5141 - 5160 of 27006 results