27,006 results
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Plasmid#140980PurposeEncodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGAlphasS-RLuc8
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
5xNF-kb_RE::RedF
Plasmid#124530PurposeNFKB RedFirefly luciferase reporterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNF-κβ RedF reporter
UseLuciferase and Synthetic BiologyExpressionMammalianAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 CMV sTurboID (C)-Arrestin-GFP
Plasmid#153010PurposeExpresses split-TurboID C-terminal fragment fused to arrestinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsTurboID (C)(G74)-Arrestin
TagsGFPExpressionMammalianPromoterCMVAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF-GalNAc-T2-msGFP2
Plasmid#140164PurposeExpresses the Golgi marker GalnacT2-msGFP2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGalNAc-T2-msGFP2 (GALNT2 Human)
ExpressionMammalianPromoterEF-1aAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
dead Sa Cas9
Plasmid#138162PurposeFor orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdSaCas9
TagsNLSExpressionMammalianMutationD10A + N580APromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLAMP1-miRFP670nano
Plasmid#127435PurposeLAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein miRFP670nanoDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLAMP1-miRFP670nano
TagsmiRFP670nanoExpressionMammalianPromoterCMVAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-TIS11B
Plasmid#121157PurposeMammalian expression of GFP tagged TIS11BDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertZFP36L1 (TIS11B) (ZFP36L1 Human)
ExpressionMammalianAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-TIS11B
Plasmid#121140PurposeMammalian expression of mCherry tagged TIS11BDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertZFP36L1 (TIS11B) (ZFP36L1 Human)
ExpressionMammalianAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-RAB-ICUE
Plasmid#118480PurposeddRFP-based single-color biosensor for monitoring cAMP dynamics.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRAB-ICUE
ExpressionMammalianPromoterCMVAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
hGATA-1 WT
Plasmid#118352PurposeThis plasmid expresses human GATA-1 WT.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthGATA-1 (GATA1 Human)
Tagsmyc and 6xHISExpressionMammalianMutationWTAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEBTet_NAD-Snifit-mito
Plasmid#113916PurposePlasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNAD-Snifit
ExpressionMammalianPromoterCMV TetO2Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-Flag-PGC-1alpha1
Plasmid#45501DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPGC-1alpha Isoform1 (Ppargc1a Mouse)
Tags6xHis and FlagExpressionMammalianMutationQ339K relative to NM_008904Available sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA 3.1 SIRT1-FL
Plasmid#105670PurposeMammalian expression construct of mouse SIRT1 full length isoformDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSIRT1 (Sirt1 Mouse)
ExpressionMammalianMutationSynonymous base changes not affecting the protein…PromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
xCas9(3.6)-BE3
Plasmid#108385PurposeMammalian expression vector for xCas9(3.6)-BE3DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertxCas9(3.6)-BE3
TagsNLSExpressionMammalianMutationE108G, S217A, A262T, S409I, E480K, E543D, M694I, …Available sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
VPR-ps-dSpCas9
Plasmid#102855PurposeA single-chain light-controllable dSpCas9 with pdDronpa1.2 domainsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdSpCas9, pdDronpa1.2
UseCRISPR and Synthetic BiologyTagsNLS and VP64-p64-Rta transcription activatorExpressionMammalianPromoterPGKAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
CRY2-mCherry-Miro1TM
Plasmid#102247PurposeExpresses fusion of CRY2PHR with mCherry and transmembrane domain of Miro1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianPromoterCMVAvailable sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK5 HA-Nprl2
Plasmid#99709PurposeMammalian expression of HA-Nprl2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNprl2 (NPRL2 )
TagsHAPromoterCMVAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMOD_B2303
Plasmid#91068PurposeModule B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35SDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
UseCRISPRPromoterCmYLCVAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
Plasmid#92390Purposeexpresses Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA component in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
ExpressionMammalianPromoterCMVAvailable sinceJune 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MCS+
Plasmid#90412PurposeA FRET sensor that report the electric potential at the inner leaflet of cell plasma membraneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLck10+ Venus+mcherry+ strech of positively charged amino acids
ExpressionMammalianAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by