27,006 results
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Plasmid#163681PurposeFar-red fluorescent calcium indicator FR-GECO1aDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFR-GECO1a
ExpressionMammalianAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPP394
Plasmid#158800PurposePlasmid containing Homo sapiens codon optimized CasΦ-1 and a SapI-GG stuffer spacer for editing in human cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCasPhi-1
ExpressionMammalianAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-eA3A-BE5-NG-BlastR
Plasmid#152998PurposeHigh purity C-to-T base editorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteA3A-BE4 T44D+S45A-nSpCas9-UGI-UGI
UseCRISPRExpressionMammalianPromoterCMVAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1.Gαi3-LgB91
Plasmid#134359PurposeNanoluc complementation assay. Expression of Gαi3 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi3. Addition of the HA epitope at N terminus of Gαi3.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGαi3-LgB91 (GNAI3 Human)
TagsHAExpressionBacterial and MammalianPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GGamma1-GFP2
Plasmid#140989PurposeEncodes a G gamma subunit (GNGT1) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGGamma1-GFP2 (GNGT1 Human)
TagsGFP2 and GSAG linkerExpressionMammalianPromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pK18-hACE2
Plasmid#149449PurposeExpresses human ACE2 driven by the K18 promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAngiotensin-converting enzyme 2 (ACE2 Human)
ExpressionMammalianPromoterK18Available sinceJune 1, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pmCherry-C1ab-Prkd1
Plasmid#139314PurposeDiacylglycerol biosensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry:Mus musculus Prkd1(138-343) (Prkd1 Mouse)
ExpressionMammalianMutationT208A, N313DAvailable sinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTfR-mNeonGreen
Plasmid#129608PurposeIn vivo visualization of Transferrin receptors (can be used for colocalization studies)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTransferrin
ExpressionMammalianPromoterCMVAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMGS36 (GFP-ARF16-PB1)
Plasmid#126581PurposeConstruct to express GFP-ARF16-PB1 under the control of the CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOsARF16-PB1 domain
TagsGFPExpressionMammalianAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
SaABEmax
Plasmid#119814PurposeA-to-G base editorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertecTadA-ecTadA*(7.10)-nSaCas9
ExpressionMammalianAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330-LMNA-gRNA1
Plasmid#122507Purposeexpresses WT spCas9 and a chimeric gRNA targeting human LMNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLMNA-gRNA1
UseCRISPRExpressionMammalianPromoterU6Available sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSF4 TetCMV intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA
Plasmid#119945PurposeExpresses SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeatsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRenilla luciferase
Tags24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …ExpressionBacterial and MammalianPromoterTetCMVAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAVS1-Neo-CAG-M2rtTA-H2BGFP (donor AB)
Plasmid#85798PurposedonorAB targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP expression systemDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPPP1R12C (PPP1R12C Human)
UseHuman targetingMutationhomology arms for knock-in into first intronAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TNFR2-GFP
Plasmid#111207Purposefluorescent fusion proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTNFRSF1B (TNFRSF1B Human)
TagsEGFPExpressionMammalianMutationS175P, D306GAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZ M2rtTA_CAGG TetON-Sox10
Plasmid#115240PurposeRMCE donor vector for inducible expression of SOX10 constitutive expression of m2rtTA (generated from plasmid #112668)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSOX10 (SOX10 Human)
ExpressionMammalianAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-NLS-AcrIIA4
Plasmid#113037PurposeExpression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSV40 NLS-AcrIIA4
UseCRISPR and Synthetic BiologyExpressionMammalianAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGW1-mCherry-EGFP-PIM
Plasmid#111759PurposeHigh expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 additionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP
ExpressionMammalianMutationVal24Glu, Tyr80Cys, Ala95Thr mutations in second …PromoterCMVAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEBTet_NADP-Snifit-mito
Plasmid#113919PurposePlasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNADP-Snifit
ExpressionMammalianPromoterCMV TetO2Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-29: LMNB1-mTagRFP-T
Plasmid#114403PurposeHomology arms and mTagRFPT-linker sequence for N-terminus tagging of human LMNB1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLMNB1 Homology Arms with mTagRFPT-linker (LMNB1 Human)
UseCRISPR; Donor templateTagsmTagRFPT-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available sinceSept. 4, 2018AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
tdTomato-KIF1A
Plasmid#102850PurposeOverexpresses KIF1A and cell fill TdTomatoDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKIF1A (Kif1a Rat)
TagstdTomatoExpressionMammalianPromoterCMVAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only