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30,881 results

Showing: 7541 - 7560 of 30881 results

  1. pCB-2

    Plasmid
    #210388
    Purpose
    All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces
    Depositor
    Gilles van Wezel
    Publication
    Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9
    CymR
    sgRNA cassette
    Use
    CRISPR; Integrative vector for use in streptomyces
    Mutation
    BsaI-flanked RFP cassette inserted in place of sp…
    Promoter
    SP11 and SP30
    Available since
    Jan. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    210388-plasmid-map-sequence-id-418474

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  9. pcDNA3.1-ERBB2-V5/HIS

    Plasmid
    #201103
    Purpose
    expression of human ERBB2 receptor tyrosine kinase in mammalian cells
    Depositor
    Pavel Krejčí
    Publication
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human ERBB2 receptor tyrosine kinase, full length, wildtype (ERBB2 Human)
    Tags
    V5/His
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    201103-plasmid-map-sequence-id-394492

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  10. pSL3718 (pQCascade_Pse)

    Plasmid
    #200899
    Purpose
    Expresses human codon-optimized Pseudoalteromonas Cas7, Cas8, Cas6, and TniQ from a CMV promoter, in a polycistronic vector format separated by T2A sequences.
    Depositor
    Samuel H. Sternberg
    Publication
    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PseCas7, PseCas8, PseCas6, PseTniQ
    Tags
    NLS (TniQ) and NLS, T2A (Cas7, Cas8, Cas6)
    Expression
    Mammalian
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    200899-plasmid-map-sequence-id-393105

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  11. TRUPATH Triple Gagust

    Plasmid
    #196054
    Purpose
    Encodes a G alpha subunit (GNAT3) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAlphaGustducin-RLuc8 (GNAT3 Human)
    GGamma1-GFP2 (GNGT1 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    196054-plasmid-map-sequence-id-393090

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  13. pHrD-IRES-Fluc

    Plasmid
    #194250
    Purpose
    Human RNAP1 promoter region driving firefly luciferase expression from IRES. Constructed by Ghoshal, et al. J Biol Chem (2004). Please cite this original manuscript!
    Depositor
    Samson Jacob
    Publication
    McCool et al RNA. 2022 Nov 2. pii: rna.079149.122. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Firefly luciferase
    Use
    Luciferase
    Promoter
    human rDNA promoter and beginning of 5' ETS …
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    194250-plasmid-map-sequence-id-391338

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  14. HA-APP-mGFP

    Plasmid
    #196696
    Purpose
    Expresses APP695 with an N-terminal HA tag and a C-terminal TagGFP2 for single molecule tracking and other measusements in mammalian cells
    Depositor
    Martino Calamai
    Publication
    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Amyloid Precursor Protein 695 isoform (APP Human)
    Tags
    HA and TagGFP2
    Expression
    Mammalian
    Available since
    April 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    196696-plasmid-map-sequence-id-391081

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  15. pCAGGS SARS-CoV-2 XBB Spike

    Plasmid
    #195287
    Purpose
    Expresses codon-optimized full length SARS-CoV-2 XBB Spike
    Depositor
    Marceline Côté
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 XBB Spike (S )
    Expression
    Mammalian
    Mutation
    Contains the following mutations: T19I, LPPA24-27…
    Promoter
    chicken β-actin promoter, CMV enhancer
    Available since
    Feb. 8, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    195287-plasmid-map-sequence-id-384139

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  17. FEMP

    Plasmid
    #191973
    Purpose
    FRET-based indicator of ER-mitochondria proximity (FEMP)
    Depositor
    Luca Scorrano
    Publication
    Naon et al Proc Natl Acad Sci U S A. 2016 Oct 4;113
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AkapYFP-Tav2a-CFP-sac1
    Expression
    Mammalian
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    191973-plasmid-map-sequence-id-379638

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Showing: 7541 - 7560 of 30881 results