Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

30,881 results

Showing: 7881 - 7900 of 30881 results

  2. BRCA1-plvxt

    Plasmid
    #119281
    Purpose
    Doxycycline-inducible human BRCA1
    Depositor
    Joan Massague
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BRCA1 (BRCA1 Human)
    Expression
    Mammalian
    Promoter
    TRE
    Available since
    Jan. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    119281-plasmid-map-sequence-id-237541

    Enlarge

  4. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA

    Plasmid
    #134925
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.
    Depositor
    Tomoji Mashimo
    Publication
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134925-plasmid-map-sequence-id-267605

    Enlarge

  5. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA

    Plasmid
    #134922
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.
    Depositor
    Tomoji Mashimo
    Publication
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134922-plasmid-map-sequence-id-267588

    Enlarge

  7. pDONR221_SLC19A1

    Plasmid
    #132266
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Publication
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SLC19A1 (SLC19A1 Human)
    Expression
    Mammalian
    Available since
    Nov. 19, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    132266-plasmid-map-sequence-id-263654

    Enlarge

  8. pDONR221_SLC27A4

    Plasmid
    #132283
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Publication
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SLC27A4 (SLC27A4 Human)
    Expression
    Mammalian
    Available since
    Nov. 14, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    132283-plasmid-map-sequence-id-263267

    Enlarge

  9. pDONR221_SLC44A2

    Plasmid
    #132214
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Publication
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SLC44A2 (SLC44A2 Human)
    Expression
    Mammalian
    Available since
    Nov. 4, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    132214-plasmid-map-sequence-id-261918

    Enlarge

  10. Calreticulin-SuperNova-KDEL

    Plasmid
    #131857
    Purpose
    Expresses SuperNova fused to ER protein calreticulin and KDEL ER-retention signal
    Depositor
    Geert van den Bogaart
    Publication
    Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SuperNova
    Tags
    KDEL ER-retention signal and Signal sequence of h…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    131857-plasmid-map-sequence-id-261768

    Enlarge

  14. CA-Stat3-t2A-mCherry

    Plasmid
    #127540
    Purpose
    Expresses constitutively active (CA) mouse Stat3 and mCherry via t2A linker.
    Depositor
    Jennifer Mitchell
    Publication
    Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Stat3 (Stat3 Mouse)
    Expression
    Mammalian
    Mutation
    A662C and N664C. Residues changed to Cys renders …
    Promoter
    CMV
    Available since
    July 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127540-plasmid-map-sequence-id-251258

    Enlarge

  16. LbCas12a-2C-NLS in pCSDest

    Plasmid
    #126638
    Purpose
    Expresses LbCas12a-2C-NLS in mammalian cell
    Depositor
    Scot Wolfe
    Publication
    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LbCas12a-2C-NLS
    Use
    CRISPR
    Tags
    3xHuman influenza hemagglutinin (HA)-Tag
    Expression
    Mammalian
    Promoter
    CMV IE94 promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126638-plasmid-map-sequence-id-247967

    Enlarge

  17. pCMV-OptoTGFBRs

    Plasmid
    #118942
    Purpose
    Mammalian expression plasmid of OptoTGFBRs (optogenetically-activated TGFb receptors)
    Depositor
    Won Do Heo
    Publication
    Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OptoTGFBRs (Tgfb1, Tgfbr2 Mouse, Mustard Weed)
    Tags
    tdTomato
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    118942-plasmid-map-sequence-id-247889

    Enlarge

  18. pcDNA3.1D/mEGFP + CrPV IGR IRES-nLuc-3XFLAG-CL1PEST

    Plasmid
    #127332
    Purpose
    Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CrPV IRES
    Depositor
    Jeremy Wilusz
    Publication
    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nanoLuciferase
    Tags
    3xFLAG and CL1/PEST
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127332-plasmid-map-sequence-id-247773

    Enlarge

  19. pDmelOR-mRho.V5.mER.hOr47a

    Plasmid
    #126478
    Purpose
    Human codon-optimized D. melanogaster Orco (hOrco) and Or47a (hOr47a). Or47a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells
    Depositor
    Bill Hansson
    Publication
    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Or47a (Or47a Fly)
    Orco (Orco Fly)
    Tags
    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens and codon optim…
    Promoter
    CMV
    Available since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    126478-plasmid-map-sequence-id-246565

    Enlarge

  20. aCat TL1 (1-907 mTFP1-TSmod-Venus STOP)

    Plasmid
    #101297
    Purpose
    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.
    Depositor
    Alpha Yap
    Publication
    Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    alpha-Catenin
    Expression
    Mammalian
    Mutation
    Contains amino acids 1-906 of alpha-Catenin
    Available since
    June 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101297-plasmid-map-sequence-id-246082

    Enlarge

Showing: 7881 - 7900 of 30881 results