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30,881 results

Showing: 8281 - 8300 of 30881 results

  1. pGinB-8X(GGS)-dCas9-FLAG-NLS

    Plasmid
    #81205
    Purpose
    CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus
    Depositor
    David Liu
    Publication
    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pGinB-8X(GGS)-dCas9-FLAG-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    81205-plasmid-map-sequence-id-154468

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  6. pEGFP-N1-mt-ro1GFP

    Plasmid
    #82407
    Purpose
    Expression of redox sensitive GFP with a mitochondrial targeting sequence in mammalian cells
    Depositor
    S. James Remington
    Publication
    Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ro1GFP
    Tags
    leader sequence of the E1α subunit of pyruvate de…
    Expression
    Mammalian
    Mutation
    C48S/F64L/T65S/S147C/Q204C
    Promoter
    CMV
    Available since
    Sept. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    82407-plasmid-map-sequence-id-161603

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  7. pTre-Tight-luciferase-GFP (467)

    Plasmid
    #65491
    Purpose
    negative control for expression assays
    Depositor
    Roderic Guigo, Rory Johnson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    tight TRE promoter
    Available since
    Sept. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65491-plasmid-map-sequence-id-148973

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  8. CIBN-GFP-TMD

    Plasmid
    #79571
    Purpose
    expression of CIBN domain fused to GFP tagged mouse Syntaxin1 TMD domain (transmembrane domain and polybasic region)
    Depositor
    Pietro De Camilli, Olof Idevall-Hagren
    Publication
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    syntaxin 1A (Stx1a Mouse)
    Tags
    CIBN and EGFP
    Expression
    Mammalian
    Mutation
    aa 249_288 (C-terminal region (polybasic stretch …
    Promoter
    CMV
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    79571-plasmid-map-sequence-id-456519

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  11. pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB-Zeo

    Plasmid
    #78352
    Purpose
    Expresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.
    Depositor
    Michael Elowitz
    Publication
    Bintu et al Science. 2016 Feb 12;351(6274):720-4. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H2B-mCherry-T2A-rTetR-KRAB
    Use
    Synthetic Biology
    Tags
    rTetR
    Expression
    Mammalian
    Promoter
    pEF alpha
    Available since
    July 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    78352-plasmid-map-sequence-id-146664

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  13. CAG sHRPb-FKBP-post mGRASP

    Plasmid
    #73146
    Purpose
    Small fragment of split HRP and FKBP fused to the extracellular terminus of the "post mGRASP" scaffold published by Magee, in which the majority of the NLG1 extracellular domain is deleted.
    Depositor
    Alice Ting
    Publication
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sHRPb-FKBP-post mGRASP
    Tags
    HA tag
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73146-plasmid-map-sequence-id-134519

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  14. 190_SMAD4 in pmiRGlo

    Plasmid
    #78128
    Purpose
    luciferase reporter for miRNA activity
    Depositor
    Heidi Schwarzenbach
    Publication
    Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SMAD4 (SMAD4 Human)
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    3'UTR containing miRNA binding sites
    Promoter
    PGK
    Available since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    78128-plasmid-map-sequence-id-146865

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  16. Rlucrarr3-Flash2

    Plasmid
    #74130
    Purpose
    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 139 of Arrestin3.
    Depositor
    Louis Luttrell
    Publication
    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Arrestin3
    Tags
    Renilla luciferase (rLuc)
    Expression
    Mammalian
    Mutation
    Insertion of FlAsH motif (CCPGCC) following amino…
    Available since
    April 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  19. pGL4.10-VEGFprom(-500 to -1)

    Plasmid
    #66130
    Purpose
    Luciferase-based reporter for VEGF promoter region (-500 to -1 bp)
    Depositor
    David Mu
    Publication
    Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Vascular Endothelial Growth Factor-A promoter region (VEGFA Human)
    Use
    Luciferase
    Promoter
    VEGF -500 to -1
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    66130-plasmid-map-sequence-id-117222

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Showing: 8281 - 8300 of 30881 results