30,881 results
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Plasmid#100550PurposeExpresses ciCas9 in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertciCas9
UseCRISPR; Frt, flp-inTagsFLAGExpressionMammalianAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG MCP-VP64
Plasmid#92357PurposeCAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMCP-VP64
UseCRISPRExpressionMammalianPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCI Cas9 H2B-RFP
Plasmid#92397PurposeCAG-driven ubiquitous expression of Cas9 with Histone2B-RFP reporter controlled by IRESDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9
UseCRISPRExpressionMammalianPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP-C2-hSphK2
Plasmid#84370PurposeExpression of AcGFP-SphK2 fusion protein in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSPHK2 (SPHK2 Human)
TagsAcGFPExpressionMammalianPromoterCMVAvailable sinceAug. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
NRCoC
Plasmid#74300PurposeMammalian expression of full-length treacle fused to GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserttreacle ribosome biogenesis factor 1 (Tcof1 Mouse)
TagsGFPExpressionMammalianPromoterCMVAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
sgRNA 1 GAPDH
Plasmid#83808PurposeExpress Sg-1 sgRNA targeting GAPDHDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSgRNA
UseCRISPRAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
MCS Lck only
Plasmid#90411PurposeA control of MCS+ that associated with cell plasma membrane only by one hydrophobic interaction at the N terminus of the FRET pair.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLck10+ Venus+mcherry
ExpressionMammalianAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX335-EN475
Plasmid#86231PurposeFor transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN477.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertspCas9-nickase and sgRNA against mouse CTCF STOP Codon
UseMouse TargetingExpressionMammalianAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZS2oxySp-GFP-proD-oxyR
Plasmid#78224Purposesynthetic gene circuitDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsynthetic gene circuit
Available sinceMay 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
Sirius-mT-Sapphire_pcDNA3
Plasmid#89584PurposeGenetically encoded ratiometric fluorescent thermometerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSirius-mT-Sapphire
ExpressionMammalianPromoterCMVAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLinker-AID-3xHA-HXGPRT-LoxP
Plasmid#86553Purposeused for a TIR1-AID system with CRISPR tagging technology in Toxoplasma gondiiDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLinker-AID-3xHA-HXGPRT 3'UTR
UseExpression in toxoplasma gondiiAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTagBFP-Akt1
Plasmid#86594PurposeExpresses TagBFP-tagged human Akt1(WT)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAkt1 (AKT1 Human)
ExpressionMammalianAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
p-EGFP-C1-Flag-mFmr1(A)
Plasmid#87913PurposeExpresses mouse FMRP-EGFP fusion with S499A to prevent phosphorylation.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFmr1 (Fmr1 Mouse)
TagsFlag and eGFPExpressionMammalianMutationS499AAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
p5M-PR-A
Plasmid#89124Purposemammalian expression of full-length human progesterone receptor-A in pCMV5 with 3 restriction sites deleted in polylinkerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman progesterone receptor-A (PGR Human)
ExpressionMammalianPromoterCMVAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p5M-hPR-B
Plasmid#89114Purposemammalian expression of full-length human progesterone receptor-B in pCMV5 with 5 restriction sites deleted in polylinkerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman progesterone receptor-B (PGR Human)
ExpressionMammalianMutation5 restriction sites deleted in polylinkerPromoterCMVAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3/hArf1(WT)-mCherry
Plasmid#79419PurposeExpresses C-terminally mCherry-tagged Arf1(WT) in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertArf1 (ARF1 Human)
TagsmCherryExpressionMammalianAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-hARdelta538-614
Plasmid#89107Purposemammalian expression of human androgen receptor with deletion of amino acids 538-614, deletion of AR DNA binding domainDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthAR-538-614 (AR Human)
ExpressionMammalianMutationhAR delta 538-614PromoterCMVAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-MYO10-HMM-Nanotrap
Plasmid#87255PurposeExpression plasmid to perform NanoSPD 2.0 assays in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMYO10 (Myo10 Mouse)
TagsGFP-nanotrapExpressionMammalianMutationMyo10 is truncated to contain aa.1-941PromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFP
Plasmid#86987PurposeFor cloning and expression of sgRNA together with expression of Cas9 and TagRFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9
ExpressionMammalianPromoterCAGAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Flag Rab12 T56N
Plasmid#86789Purposemammalian expression of Rab12 T56N, constitutively GDP-bound mutantDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRab12 (RAB12 Human)
TagsFlagExpressionMammalianMutationT56N, constitutively GDP-bound mutantPromoterCMVAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only