Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

30,881 results

Showing: 9381 - 9400 of 30881 results

  2. StrKDEL-IRES-ManII-mScarlet-i

    Plasmid
    #117274
    Purpose
    Expresses an Str-KDEL Hook for the RUSH system and separately mannosidase II-mScarlet-i
    Depositor
    David Stephens
    Publication
    McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    partial sequence of mannosidase II (Man2a1 Mouse)
    Streptavidin-KDEL
    Tags
    mScarlet-i
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 116 of ManII
    Promoter
    CMV and IRES
    Available since
    Jan. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    117274-plasmid-map-sequence-id-233028

    Enlarge

  3. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.4) (RTW1028)

    Plasmid
    #114084
    Purpose
    CAG promoter expression plasmid for human codon optimized enAsBE1.4
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.4)
    Tags
    2x SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A, human codon optimi…
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114084-plasmid-map-sequence-id-230536

    Enlarge

  10. TagBFP2-INPP4BC842A-CAAX

    Plasmid
    #116858
    Purpose
    inactive plasma membrane targetted PI(3,4)P2 phosphatase
    Depositor
    Gerry Hammond
    Publication
    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    INPP4B (INPP4B Human)
    Tags
    HRAS(172-189) and TagBFP2
    Expression
    Mammalian
    Mutation
    catalytic cysteine 842 changed to alanine
    Available since
    Oct. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    116858-plasmid-map-sequence-id-224772

    Enlarge

  14. pEYFP-N1-eKR2

    Plasmid
    #115337
    Purpose
    Expresses enhanced sodium pump for light-driven extrusion of sodium; activation with green light; codon-optimized for human/mouse
    Depositor
    Peter Hegemann
    Publication
    Grimm et al Sci Rep. 2018 Jun 18;8(1):9316. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eKR2
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    115337-plasmid-map-sequence-id-222075

    Enlarge

  16. pTfR-mScarlet-I

    Plasmid
    #112954
    Purpose
    In vivo visualization of Transferrin receptors (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pTfR
    Tags
    mScarlet-I
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    112954-plasmid-map-sequence-id-327653

    Enlarge

  17. pcDNA3-Venus-173-N-ORAI1

    Plasmid
    #87618
    Purpose
    N-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.
    Depositor
    Jin Zhang
    Publication
    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Venus173-N-ORAI1 (ORAI1 Human, Synthetic)
    Tags
    6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87618-plasmid-map-sequence-id-176683

    Enlarge

Showing: 9381 - 9400 of 30881 results