Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

30,881 results

Showing: 11521 - 11540 of 30881 results

  2. pcDNA5-FRT-TO-Nsp16-HF

    Plasmid
    #157725
    Purpose
    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Publication
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nsp16-HF (ORF1ab SARS-CoV-2)
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized
    Available since
    Aug. 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157725-plasmid-map-sequence-id-307930

    Enlarge

  3. pCX-Lyn-SpiCee

    Plasmid
    #140837
    Purpose
    mammalian expression of Lyn-SpiCee, a lipid raft-targeted variant of the calcium buffer SpiCee that prevents downstream signaling
    Depositor
    Xavier Nicol
    Publication
    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyn-SpiCee
    Tags
    mRFP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140837-plasmid-map-sequence-id-304814

    Enlarge

  4. pCX-SpiCee-Kras

    Plasmid
    #140838
    Purpose
    mammalian expression of SpiCee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the calcium buffer SpiCee that prevents downstream signaling
    Depositor
    Xavier Nicol
    Publication
    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpiCee-Kras
    Tags
    mRFP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140838-plasmid-map-sequence-id-304816

    Enlarge

  7. p2,0_3M5M_eGFP

    Plasmid
    #135474
    Purpose
    Encodes Marburg virus minigenome with eGFP reporter gene under control of a T7 RNA polymerase promoter
    Depositor
    Elke Mühlberger
    Publication
    Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Marburg virus minigenome, eGFP reporter
    Expression
    Mammalian
    Mutation
    None
    Promoter
    T7
    Available since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    135474-plasmid-map-sequence-id-268921

    Enlarge

  8. p2,0_3M5M_luciferase

    Plasmid
    #135475
    Purpose
    Encodes Marburg virus minigenome with luciferase reporter gene under control of a T7 RNA polymerase promoter
    Depositor
    Elke Mühlberger
    Publication
    Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Marburg virus minigenome, luciferase reporter
    Expression
    Mammalian
    Mutation
    None
    Promoter
    T7
    Available since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    135475-plasmid-map-sequence-id-301586

    Enlarge

  9. XLone-BSD SARS-CoV2 N P2A mCherry

    Plasmid
    #154398
    Purpose
    PiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and mCherry
    Depositor
    Xiaoping Bao
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV2 N Protein (N SARS-CoV2)
    Use
    Piggybac
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    TRE3G
    Available since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154398-plasmid-map-sequence-id-304591

    Enlarge

  11. SPDK3889(TRV-RNA2-pPEBV-MCS-AtMet-tRNA)

    Plasmid
    #149277
    Purpose
    Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs
    Depositor
    Savithramma Dinesh-Kumar
    Publication
    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Modified TRV RNA2 with PEBV promoter and AtMet-tRNA mobile RNA sequence
    Use
    T-dna vector
    Mutation
    T2318C (DNA) in PEBV coat protein sequence
    Available since
    June 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    149277-plasmid-map-sequence-id-300516

    Enlarge

  12. pGBW-m4134388

    Plasmid
    #152696
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    June 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152696-plasmid-map-sequence-id-300227

    Enlarge

  14. pGBW-m4134900

    Plasmid
    #151911
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 spike prefusion-stabilized
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 spike prefusion-stabilized (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    d1-15 d1209-1273 R682A R685G K986P V987P
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    151911-plasmid-map-sequence-id-300222

    Enlarge

  15. pHLuc Control

    Plasmid
    #141091
    Purpose
    pH-stable bioluminescent reporter that serves as the control counterpart of pHLuc; utilizes eGFP instead of SEP
    Depositor
    Jeak Ling Ding
    Publication
    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Antares-T2A-puromycin-IRESv24-eGFP-Nanoluc
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    141091-plasmid-map-sequence-id-296869

    Enlarge

  16. mApple-hCOL1A1

    Plasmid
    #140113
    Purpose
    Expresses fluorescently-tagged human Type I procollagen α1 chain with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Type I procollagen α1 (COL1A1 Human)
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    COL1A1 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available since
    May 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140113-plasmid-map-sequence-id-296772

    Enlarge

  20. pDEST40-2XHA-Src-K298M

    Plasmid
    #140314
    Purpose
    Expresses HA-tagged human Src-K298M in mammalian cells for structure-function studies
    Depositor
    Mark Moasser
    Publication
    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Src mutant (SRC Human)
    Tags
    2X HA
    Expression
    Mammalian
    Mutation
    K298M
    Promoter
    CMV
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140314-plasmid-map-sequence-id-291897

    Enlarge

Showing: 11521 - 11540 of 30881 results