30,738 results

1. pKO403_lacZ_Sp

   Plasmid

   #174726

   Purpose

   Bifidobacterium-E.coli shuttle vector for seamless gene cloning

   Depositor

   Tohru Suzuki

   Article

   Altaib et al Microbiol Resour Announc. 2022 Jan 20;11

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST17-UbE2G2

   Plasmid

   #15791

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2G2 (UBE2G2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. NowGFP /pQE-30

   Plasmid

   #74749

   Purpose

   NowGFP gene for bacterial expression

   Depositor

   Konstantin Lukyanov

   Article

   Sarkisyan et al Biophys J. 2015 Jul 21;109(2):380-9. doi

   Insert

   NowGFP
   

   Tags

   HisTag (with a mutation but still working)

   Expression

   Bacterial

   Mutation

   WasCFP with substitutions E6K, L42M, T43S, V68M, …

   Promoter

   T5

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVoPo-04

   Plasmid

   #187839

   Purpose

   Suicide vector to generate markerless gene deletions in Fusobacterium nucleatum

   Depositor

   Jörg Vogel

   Article

   Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOPINB-OTULIN (full-length, aa 1-352)

   Plasmid

   #61464

   Purpose

   Expresses human OTULIN (full-length) in E. coli.

   Depositor

   David Komander

   Article

   Keusekotten et al Cell. 2013 Jun 6;153(6):1312-26. doi: 10

   Insert

   OTULIN (OTULIN Human)
   

   Tags

   His6-3C

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSIBR004

   Plasmid

   #177667

   Purpose

   SIBR Intron 4 (Int4) interrupts the CDS of FnCas12a

   Depositor

   John van der Oost

   Article

   Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   lacUV5

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOSIP-KT (KanR, P21)

   Plasmid

   #45987

   Depositor

   Keith Shearwin, Drew Endy

   Article

   St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBXNH3CA_MBP

   Plasmid

   #132700

   Purpose

   expresses Avi-tagged MBP in E.coli, to use as positive control in ELISA

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

   Insert

   Maltose binding Protein
   

   Tags

   Avi and His

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pH6HTC_FAM98A-Halo

   Plasmid

   #175329

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   FAM98A (FAM98A Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Available Since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHT-6xR.ads-PxylA-DI

    Plasmid

    #47386

    Purpose

    Expresses ads under Pgrac promoter (inducible by IPTG) and expresses dxs and idi under PxylA promoter (inducible by xylose) in bacteria

    Depositor

    Heng-Phon Too

    Article

    Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

    Inserts

    ads
    dxs
    idi
    

    Tags

    6xR and His

    Expression

    Bacterial

    Promoter

    Pgrac and PxylA

    Available Since

    Oct. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pE6n

    Plasmid

    #17460

    Purpose

    modified pENTR vector for N-terminal EYFP tag fusion with your gene of interest

    Depositor

    Giovanna Benvenuto

    Article

    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

    Type

    Empty backbone

    Use

    Modified gateway entry vector

    Tags

    EYFP

    Expression

    Bacterial, Insect, Mammalia…

    Available Since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLPO1

    Plasmid

    #209975

    Purpose

    Contains Level 0 Part: replication origin (256rep) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    replication origin (256_rep)
    

    Expression

    Bacterial

    Available Since

    Sept. 13, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pQTEV-ANP32A

    Plasmid

    #31563

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    acidic (leucine-rich) nuclear phosphoprotein 32 family, member A (ANP32A Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-28a-hPKM1

    Plasmid

    #44241

    Depositor

    Lewis Cantley, Matthew Vander Heiden

    Article

    Christofk et al Nature. 2008 Mar 13;452(7184):181-6. doi

    Insert

    Pyruvate Kinase M1 (PKM Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJM100 (pUC18T-miniTn7T-gm-araC-ParaBAD)

    Plasmid

    #110554

    Purpose

    miniTn7 delivery plasmid with araC-ParaBAD inducible promoter

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    araC-ParaBAD
    

    Expression

    Bacterial

    Promoter

    AraC-ParaBAD

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBiex-1 GFPNanobody_SNAP

    Plasmid

    #82711

    Purpose

    Expresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

    Insert

    GFP Nanobody
    

    Tags

    6xHis Purification tag, FLAG purification tag, an…

    Expression

    Bacterial and Insect

    Promoter

    T7 Promoter

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-Ran(Q69L)

    Plasmid

    #42048

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    Ran (RAN Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Q69L

    Available Since

    Feb. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21a-GluRS-His

    Plasmid

    #124109

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Glutamyl-tRNA ligase (gltX Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET51b-His-TEV-SNAP-tag

    Plasmid

    #167269

    Purpose

    Overexpression of SNAP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAP-tag
    

    Tags

    His x10

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSNAP-tag (T7) Vector

    Plasmid

    #101137

    Purpose

    Expression of protein of interest with SNAP-Tag in bacterial cells

    Depositor

    New England Biolabs, Ana Egana

    Type

    Empty backbone

    Tags

    SNAP-tag (SNAP26b)

    Expression

    Bacterial

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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