30,738 results

1. pRSET-mEosEM

   Plasmid

   #132708

   Purpose

   application in Correlative light-electron microscopy

   Depositor

   Pingyong Xu

   Article

   Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   mEosEM
   

   Expression

   Bacterial

   Available Since

   Oct. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. JC Mad-1

   Plasmid

   #25626

   Depositor

   Peter Howley

   Article

   Howley et al J Virol. 1980 Dec . 36(3):878-82.

   Insert

   JC Virus Mad-1
   

   Expression

   Bacterial

   Available Since

   Oct. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

3. human p53-(82-393)

   Plasmid

   #24867

   Depositor

   Cheryl Arrowsmith

   Article

   Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

   Insert

   p53 (TP53 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains aa 82-393

   Available Since

   Aug. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pKAR5

   Plasmid

   #149461

   Purpose

   Broad-Host range expression vector, RK2 and pUC origin, AraC activator/repressor, PBAD promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   AraC-mRFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PBAD

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTriEx4-Cdc42-2G

   Plasmid

   #68814

   Purpose

   FRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)

   Depositor

   Olivier Pertz

   Article

   Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

   Insert

   Cdc42 activation reporter
   

   Tags

   His and Myc

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   CMV

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSBinit

   Plasmid

   #110100

   Purpose

   E.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal myc and 6xHisTag

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

   Type

   Empty backbone

   Tags

   myc, His

   Expression

   Bacterial

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTrcHIS-wt7D12

   Plasmid

   #125268

   Purpose

   Expresses wild type 7D12 anti-EGFR nanobodies

   Depositor

   Angus Johnston

   Article

   Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

   Insert

   7D12 nanobody
   

   Tags

   His-Tag

   Expression

   Bacterial

   Promoter

   Trc

   Available Since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. bdSUMO-GFP-HiBit

   Plasmid

   #170989

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-muGFP-HiBiT
   

   Use

   Luciferase

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. TOPO mCherry

   Plasmid

   #68490

   Purpose

   TOPO construct expressing mCherry for generation of GMAP-compatible gene

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   mCherry
   

   Use

   Gmap

   Expression

   Bacterial

   Promoter

   Plac

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAIP4_3KB5

    Plasmid

    #234225

    Purpose

    Heterologous protein expression of tripartite motif-containing protein 72 (Mg53) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    3KB5 (TRIM72 Human)
    

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pTriEx-RhoA FLARE.sc Biosensor WT

    Plasmid

    #12150

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor (RHOA Human)
    

    Tags

    6xHis

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEasyCas9

    Plasmid

    #184909

    Purpose

    Expresses the Streptococcus pyogenes Cas9 protein

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCI-neo-cOVA

    Plasmid

    #25097

    Depositor

    Maria Castro

    Article

    Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

    Insert

    cytoplasmic ovalbumin
    

    Expression

    Bacterial and Mammalian

    Available Since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBP-P_tetR-PTet*

    Plasmid

    #204077

    Purpose

    Type 0 promoter part

    Depositor

    Mikhail Shapiro

    Insert

    tetR repressor and PTet* tetracycline-inducible promoter
    

    Expression

    Bacterial

    Mutation

    None

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. SpyTag-sfGFP

    Plasmid

    #184226

    Purpose

    Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.

    Depositor

    Mark Howarth

    Article

    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

    Insert

    SpyTag-sfGFP
    

    Tags

    His6, SpyTag, and TEV protease cleavage site

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. DiLiCre 2.0

    Plasmid

    #198752

    Purpose

    A Cre recombinase that is expressed upon doxycycline when combined with the UbC-blasticidine-P2A-TETon-3G transactivator. DiLiCre 2.0 is a cre recombinase that is activated by violet light.

    Depositor

    Jacco van Rheenen

    Article

    Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:

    Insert

    Cre-PhoCI-ERT2-ERT2
    

    Use

    Lentiviral

    Tags

    Cre, kept in a photocleavable site, followed by 2…

    Expression

    Bacterial and Mammalian

    Promoter

    TRE3GV

    Available Since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDuet TDP43 WT

    Plasmid

    #27462

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Coexpression

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Jan. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMBEC6

    Plasmid

    #187598

    Purpose

    Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Gentamycin resistance

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

    Insert

    nCas9-BEC-UGI; cas6f, gfp
    

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDFDuet-MKK6-EE p38alpha

    Plasmid

    #82719

    Purpose

    Expresses constitutively active human MKK6 and murine p38 alpha.

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Inserts

    MKK6 (MAP2K6 Human)
    p38alpha (Mapk14 Mouse)
    

    Expression

    Bacterial

    Mutation

    Ser207 to Glu, Thr211 to Glu

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

20. pSL1749 (pDonor, R*MmeI)

    Plasmid

    #160733

    Purpose

    Mini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST donor DNA
    

    Expression

    Bacterial

    Promoter

    N/A

    Available Since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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