29,712 results

1. pET15b His6-FKBPF36V

   Plasmid

   #73180

   Purpose

   Expressing FKBPF36V protein in washing out shield-1 ligand for FKBPdd

   Depositor

   Thomas Wandless

   Article

   Egeler et al J Biol Chem. 2011 Sep 9;286(36):31328-36

   Insert

   FKBP(F36V) (FKBP1A Human)
   

   Use

   Tags

   His 6x

   Expression

   Bacterial

   Mutation

   F36V

   Promoter

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

2. GST-mDia1(FH1FH2DAD)-His

   Plasmid

   #85822

   Purpose

   contains the formin homolgy domains: FH2 is required for nucleation, processive tracking of barbed-end of actin filaments ; FH1 permits enhancement of actin polymerization rate.

   Depositor

   Antoine Jégou, Guillaume Romet-Lemonne

   Article

   Jegou et al Nat Commun. 2013;4:1883. doi: 10.1038/nc

   Insert

   Protein diaphanous homolog 1 (Diaph1 Mouse)
   

   Use

   Tags

   8*His and GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mOrange2-pBAD

   Plasmid

   #54531

   Purpose

   Localization: Protein Expression Vector , Excitation: 549, Emission: 565

   Depositor

   Roger Tsien, Michael Davidson, Nathan Shaner

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Type

   Empty backbone

   Use

   Tags

   6xHis and mOrange2

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNCS-mCyRFP1

   Plasmid

   #84545

   Purpose

   Bacterial expression of mCyRFP1

   Depositor

   Michael Lin

   Article

   Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   mCyRFP1
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBHM2406

   Plasmid

   #173442

   Purpose

   pRS316-mNeonGreen-CBP-2XFLAG-HYGB, contains C. neoformans optimized mNeonGreen and HYG marker for use as PCR template

   Depositor

   Hiten Madhani

   Article

   Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

   Insert

   mNeonGreen-CBP-2XFLAG-HYGB
   

   Use

   Tags

   CBP-2xFLAG

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Lac-I-SceI-Tet

   Plasmid

   #17655

   Depositor

   Tom Misteli

   Article

   Soutoglou et al Nat Cell Biol. 2007 Jun . 9(6):675-82.

   Insert

   lac I-SceI tet
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 30, 2008

   Availability

   Academic Institutions and Nonprofits only

7. P25-pZE-SUMO-PbTTA

   Plasmid

   #204634

   Purpose

   ColE1 ori, KanR, TetR, Tet promoter with a codon optimized pbTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.

   Depositor

   Aditya Kunjapur

   Article

   Jones et al Commun Biol. 2023 Sep 11;6(1):929. doi:

   Insert

   His-SUMO-TEV-PbTTA
   

   Use

   Synthetic Biology

   Tags

   His Tag; SUMO Tag; TEV Protease Site

   Expression

   Bacterial

   Mutation

   Promoter

   PLtetO-1 promoter

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRAD1

   Plasmid

   #47697

   Purpose

   General cloning vector for use in E. coli or D. radiodurans

   Depositor

   Mary Lidstrom

   Article

   Meima et al Appl Environ Microbiol. 2000 Sep;66(9):3

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   repU

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCS2(+)-zGrad

   Plasmid

   #119716

   Purpose

   Plasmid to generate zGrad mRNA to inactivate proteins in zebrafish

   Depositor

   Holger Knaut

   Article

   Yamaguchi et al Elife. 2019 Feb 8;8. pii: 43125. doi: 10

   Insert

   zGrad
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GST HP1 alpha

    Plasmid

    #24074

    Depositor

    Naoko Tanese

    Article

    Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

    Insert

    HP1 alpha (CBX5 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pKDsgRNA-FRT

    Plasmid

    #183241

    Purpose

    anhydrotetracycline (aTC) inducible sgRNA that targets FRT scar sites and arabinose inducible lambda Red

    Depositor

    Karin Schnetz

    Article

    Rangarajan et al Microbiology (Reading). 2022 Apr;168(4).

    Insert

    sgRNA-FRT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P-tet

    Available since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMON-HsNMT1

    Plasmid

    #67475

    Purpose

    Expression of human N-myristoyltransferase 1 for N-myristoylation of ARF family proteins in bacteria

    Depositor

    Richard Kahn

    Article

    Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

    Insert

    HsNMT1 (NMT1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

13. SpyDock

    Plasmid

    #124618

    Purpose

    Expresses SpyDock to bind SpyTag non-covalently for affinity purification

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyDock
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    E77A mutation prevents isopeptide bond formation,…

    Promoter

    T7

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJM101(pUC18T-miniTn7T-gm-lacIq-Ptac)

    Plasmid

    #110558

    Purpose

    miniTn7 delivery plasmid with lacIq-Ptac inducible promoter

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    lacIq-Ptac
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacIq-Ptac

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX-4T-3-mR7BD

    Plasmid

    #79149

    Purpose

    bacterial expression of GST-mR7BD (Rab7 binding domain of murine RILP)

    Depositor

    Aimee Edinger

    Article

    Romero Rosales et al Mol Biol Cell. 2009 Jun;20(12):2831-40.

    Insert

    R7BD (Rilp Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 220-299

    Promoter

    tac

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pnEA-vH_His-TEV-SEPT2_SEPT6

    Plasmid

    #174497

    Purpose

    bacterial co-expression of human SEPT2 and of human SEPT6

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN2 (SEPTIN2 Human)
    SEPTIN6 (SEPTIN6 Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKL10-mRuby-iATPSnFR1.0 bb118

    Plasmid

    #158980

    Purpose

    Constitutive expression (sigma 70 promoter) of the ratiometric mRuby-iATPSnFR1.0 ATP indicator in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

    Insert

    mruby-iATPSNfR1.0
    

    Use

    Tags

    mruby3

    Expression

    Bacterial

    Mutation

    codon optimized for e. coli from Addgene#102557

    Promoter

    Bba_J23118 (sigma 70)

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX-5X-1-Full-Length-MePCE

    Plasmid

    #113539

    Purpose

    Produces an N-terminal GST-tagged full length MePCE protein.

    Depositor

    Blerta Xhemalce

    Article

    Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

    Insert

    MePCE (MEPCE Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pZS*12-MS2-GFP

    Plasmid

    #26245

    Depositor

    Philippe Cluzel

    Article

    Guet et al Nucleic Acids Res. 2008 Jul . 36(12):e73

    Insert

    MS2-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    five amino acid linker (Ser, Gly, Gly, Gly, Gly) …

    Promoter

    Available since

    Nov. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBb(RK2)1k-GFPuv

    Plasmid

    #106394

    Purpose

    IPTG-inducible trc promoter expressing gfpuv on RK2 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge