29,712 results

1. pET28b-miniE-DBCO

   Plasmid

   #181985

   Purpose

   Used to label proteins with the MiniR genetically encoded tag

   Depositor

   Kimberly Beatty

   Article

   Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

   Insert

   MiniE-DBCO
   

   Use

   Tags

   DBCO-Tag, His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcold-6xHis-HRV3Csite-HsTRBP

   Plasmid

   #41096

   Depositor

   Phillip Zamore

   Article

   Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

   Insert

   TRBP (TARBP2 Human)
   

   Use

   Tags

   6xHis-HRV3Csite

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pET11a-SUMO1

   Plasmid

   #53138

   Purpose

   E.coli expression of untagged mature human SUMO1 (amino acids 1-97)

   Depositor

   Frauke Melchior

   Article

   Pichler et al Cell. 2002 Jan 11;108(1):109-20.

   Insert

   SUMO1 (SUMO1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mature form (amino acids 1-97)

   Promoter

   T7

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRetroX-PTuner DD-M.EcoGII-v5-Telomeric repeat-binding-factor1

   Plasmid

   #122084

   Purpose

   Express M.EcoGII fused to TRF1 with detabilization domain - inducible

   Depositor

   Laure Crabbe

   Article

   Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

   Insert

   DD-linker-M.EcoGII-V5-TERF1
   

   Use

   Retroviral

   Tags

   V5 tag on Nter of TERF1

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSFDuet-1_mNeongreen

   Plasmid

   #182413

   Purpose

   Bacterial expression of mNeongreen

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   mNeongreen
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-Spike

   Plasmid

   #173921

   Purpose

   Expresses the original SARS-Cov-2 Spike Protein under a CMV Promoter

   Depositor

   Andreas Stuermer

   Insert

   SARS-2 Spike Protein (S Human, Synthetic, SARS-COV-2)
   

   Use

   Tags

   no tags, native protein

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   standard CMV

   Available since

   April 11, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pEC3079 (p7INT-Pveg_lacI)

   Plasmid

   #218505

   Purpose

   integrative plasmid enabling constitutive expression of lacI in Streptococcus, targets the attP site of phage T12 in several S. pyogenes strains via an integrase

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   lacI (lacI Escherichia coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pveg

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MBP-TEVcs(ENLYFQ/G)-His6-uTEV2Δ(220-242)-R5

   Plasmid

   #135456

   Purpose

   Bacterial expression of uTEV2Δ protease

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
   

   Use

   Tags

   MBP, His6

   Expression

   Bacterial

   Mutation

   S219V mutation improves stability, T30A/S153N mut…

   Promoter

   tac promoter

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-6xHis-(-30)GFP

   Plasmid

   #62936

   Purpose

   Expression of 6xHis-(-30)GFP in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   (-30)GFP
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUC19 Giles integrase

    Plasmid

    #170049

    Purpose

    pUC19 with Giles integrase

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Insert

    GIles Integrase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET21d-myc-BirA

    Plasmid

    #109424

    Purpose

    Bacterial expression of biotin ligase (BirA) with an N-terminal myc epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    BirA (birA )
    

    Use

    Tags

    myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PGAM5

    Plasmid

    #38976

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PGAM5 (PGAM5 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pyEvolvR (enCas9-PolI5M)

    Plasmid

    #158073

    Purpose

    Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    John Dueber

    Article

    Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

    Insert

    enCas9-PolI5M
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pT2/HB

    Plasmid

    #26557

    Depositor

    Perry Hackett

    Insert

    pT2/HB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET21b-Caspase-3

    Plasmid

    #90087

    Purpose

    Expresses wild type caspase 3 in bacterial cells

    Depositor

    Clay Clark

    Article

    Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

    Insert

    Caspase 3 (CASP3 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Wild type

    Promoter

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SIRT1.1

    Plasmid

    #13735

    Depositor

    John Denu

    Article

    Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

    Insert

    SIRT1 (SIRT1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBL6

    Plasmid

    #50549

    Purpose

    Expresses mCherry under the PLtetO-1 promoter

    Depositor

    Jeffrey Tabor

    Article

    Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET His6 TEV expression vector with BioBrick polypromoter restriction sites (14-B)

    Plasmid

    #48308

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ICMAB - pET22b T7 EncFtn Mms6

    Plasmid

    #225538

    Purpose

    Construction of intracellular magnetite accumulating bacterial systems.

    Depositor

    Urartu Seker

    Article

    Yavuz et al Small. 2022 Jul;18(26):e2200537. doi: 10

    Inserts

    Iron binding protein
    Ferroxidase
    Material binding peptite
    Iron transporter protein
    

    Use

    Tags

    no

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTU1-B-SP44s-GUS

    Plasmid

    #172430

    Purpose

    Expresses Streptomyces codon-optimised beta-D-glucuronidase

    Depositor

    Paul Freemont, Simon Moore

    Article

    Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

    Insert

    beta-D-glucuronidase
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    SP44s

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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