29,712 results

1. pBR322-Pdp1_NTD-GFP-HiBiT

   Plasmid

   #198317

   Purpose

   Loads HiBiT-tagged GFP into PVCs using Pdp1 packaging domain; also contains Pnf

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   Pdp1_NTD-GFP-HiBiT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNS207

   Plasmid

   #35041

   Depositor

   Stephen Sligar

   Article

   Nikkila et al Eur J Biochem. 1991 Dec 5;202(2):309-13.

   Insert

   cytochrome b562
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   March 2, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pBad-CA

   Plasmid

   #105665

   Purpose

   expresses thermostable variant of human carbonic anhydrase II with C-terminal 6x his tag

   Depositor

   Ryan Mehl

   Article

   Mehl et al 2020

   Insert

   human carbonic anhydrase II thermostable variant (CA2 Human)
   

   Use

   Tags

   6x his tag

   Expression

   Bacterial

   Mutation

   Promoter

   pBad/arabinose

   Available since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKM493

   Plasmid

   #140191

   Purpose

   ORBIT integrating plasmid for C-terminal tagging with cleavable EGFP.

   Depositor

   Kenan Murphy

   Article

   Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

   Insert

   TEV-Flag-Gly4-eGFP
   

   Use

   Tags

   Flag + eGFP

   Expression

   Bacterial

   Mutation

   Promoter

   PGroEL

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDF-MCM4/6

   Plasmid

   #116951

   Purpose

   Expresses human MCM4 and human MCM6

   Depositor

   James Chong

   Article

   Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

   Inserts

   Human MCM6 (MCM6 Human)
   Human MCM4 (MCM4 Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPBAD-yhjH

   Plasmid

   #111613

   Purpose

   Arabinose-inducible expression of the E. coli cyclic di-GMP phosphodiesterase gene yhjH

   Depositor

   Tim Tolker-Nielsen

   Article

   Christensen et al Infect Immun. 2013 Aug;81(8):2705-13. do

   Insert

   yhjH
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A52V

   Promoter

   Available since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpyTag-sfGFP

   Plasmid

   #184226

   Purpose

   Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.

   Depositor

   Mark Howarth

   Article

   Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

   Insert

   SpyTag-sfGFP
   

   Use

   Tags

   His6, SpyTag, and TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pComb3XLambda

   Plasmid

   #63892

   Purpose

   Backbone pComb3X containing PCR template for human antibody lambda light chain constant region

   Depositor

   Carlos Barbas

   Article

   Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

   Insert

   human antibody lambda light chain (IGLL5 Human)
   

   Use

   Phage display

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacZ

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC-GFP-HS

   Plasmid

   #133305

   Purpose

   "Burdensome" GFP expressing plasmid carrying hok/sok toxin-antitoxin for plasmid stabilisation

   Depositor

   Chris Barnes

   Article

   Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

   Insert

   hok/sok toxin-antitoxin cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB33eCPX

    Plasmid

    #23336

    Depositor

    Patrick Daugherty

    Article

    Rice et al Protein Eng Des Sel. 2008 Jul . 21(7):43

    Insert

    Enhanced Circularly Permuted Outer Membrane Protein X
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. CJ8421_04975 Rptr2 recording plasmid

    Plasmid

    #194211

    Purpose

    CJ8421_04975 Rptr2 targeted DNA sequence

    Depositor

    Chase Beisel

    Article

    Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

    Insert

    CJ8421_04975 Rptr2 DNA target
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Nkx2.1

    Plasmid

    #15540

    Depositor

    John Rubenstein

    Article

    Shimamura et al Development. 1995 Dec . 121(12):3923-33.

    Insert

    Nkx2.1 (Nkx2-1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBAD-LSSmApple

    Plasmid

    #191259

    Purpose

    Bacterial expression plasmids for Long Stokes shift red fluorescent protein LSSmApple

    Depositor

    Robert Campbell

    Article

    Ejike et al Biochemistry. 2024 Jan 2;63(1):171-180.

    Insert

    LSSmApple
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFloat.cspA_v1

    Plasmid

    #133071

    Purpose

    Entry vector for cloning genes under a cold-shock cspA promoter for expression in bacteria. Version 1.0.

    Depositor

    Kacper Rogala, David Sabatini

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    3C-His6 and His6-3C

    Expression

    Bacterial

    Mutation

    Promoter

    cspA

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPFO*

    Plasmid

    #120400

    Purpose

    Expresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.

    Depositor

    Arun Radhakrishnan

    Article

    Das et al Proc Natl Acad Sci U S A. 2013 Jun 25;11

    Insert

    Mutant form of Perfringolysin O
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Amino acids 1-27 are deleted, starts at amino aci…

    Promoter

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAP03-acc(3)IV

    Plasmid

    #69862

    Purpose

    pCAP03-acc(3)IV is a upgraded version of the pCAP01(Plasmid #59981)

    Depositor

    Bradley Moore

    Article

    Tang et al ACS Chem Biol. 2015 Oct 21.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMO9075

    Plasmid

    #117481

    Purpose

    empty complementation vector for Desulfovibrio: contains promoter from Aph(3')-IIa but no RBS, contains pBG1 and pMB1 replicons, SpecR

    Depositor

    Judy Wall

    Article

    Keller et al Methods Enzymol. 2011;497:503-17. doi: 1

    Insert

    Aph(3')-IIa promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Aph(3')-IIa promoter

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-His6MBP-A3A-Gly6His6

    Plasmid

    #187822

    Purpose

    Expression human APOBEC3A in E.coli.

    Depositor

    Hao Wu

    Article

    Fabyanic et al Nat Biotechnol. 2023 Aug 28. doi: 10.103

    Insert

    APOBEC3A (APOBEC3A Human)
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7 promoter (on the backbone)

    Available since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB6K-mcherry-iATPsnFR1.1

    Plasmid

    #227239

    Purpose

    Inducible ATP sensor for Pseudomonas putida

    Depositor

    Fuzhong Zhang

    Article

    Mu et al Nat Commun. 2024 Jun 21;15(1):5299. doi:

    Insert

    iATPsnFR1.1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEB2-E2-Crimson

    Plasmid

    #104010

    Purpose

    Plasmid encoding E2-Crimson

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    E2-Crimson
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to DsRed (R2D, S4T, K5E, E10P,…

    Promoter

    proC

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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