30,738 results

1. pET21a-HisRS-His

   Plasmid

   #124111

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Histidyl-tRNA ligase (hisS Escherichia coli)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYES2.1-TRP1

   Plasmid

   #11007

   Depositor

   Francisco Malagon

   Insert

   TRP1 (TRP1 Budding Yeast)
   

   Tags

   V5 and his6

   Expression

   Bacterial and Yeast

   Mutation

   ORF-TRP1 without stop codon

   Available Since

   Dec. 16, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-HA-AR-DBD-LBD

   Plasmid

   #171237

   Purpose

   Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain and Ligand-binding domain (DBD-LBD)

   Depositor

   Steven Balk

   Article

   Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

   Insert

   2xHA-tagged human androgen receptor trancate:DNA-binding domain and Ligand-binding domain (AR Human)
   

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   AR truncate

   Promoter

   CMV

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET22b-msfGFP-NPM1

   Plasmid

   #237670

   Purpose

   For expression of msfGFP-NPM1 in Ecoli

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   NPM1 (NPM1 Human)
   

   Tags

   msfGFP

   Expression

   Bacterial

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. T7-LacO-LgBiT

   Plasmid

   #216779

   Purpose

   Expresses LgBiT in bacteria

   Depositor

   Alex Dunn

   Article

   Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

   Insert

   LgBiT
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. NylCA-TS_pET28a

   Plasmid

   #215419

   Purpose

   Expression construct for NylCA-TS gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylCA-TS
   

   Expression

   Bacterial

   Mutation

   S111G/A137L/E263Q

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEVOL-ABK

   Plasmid

   #126035

   Purpose

   Efficient expression of tRNA synthetase/tRNA for the incorporation of a photo-cross-linker, 3’-azibutyl-N-carbamoyl-lysine (AbK) into recombinant protein by amber codon (TAG) suppression in E. coli.

   Depositor

   Andrea Musacchio

   Article

   Pan et al Nat Commun. 2019 Sep 6;10(1):4046. doi:

   Inserts

   tRNA synthetase 1
   tRNA synthetase 2
   tRNA for TAG codon
   

   Expression

   Bacterial

   Mutation

   L274M, C313A, Y349F

   Promoter

   araBAD promoter, glnS promoter, and proK promoter

   Available Since

   Sept. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJL1-CRM197-4xDQNAT

   Plasmid

   #128395

   Purpose

   In vitro expression of CRM197 with C terminal 4xDQNAT glycosylation sites

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Diphtheria toxin
   

   Tags

   4xDQNAT glycosylation tag and 6xHis tag

   Expression

   Bacterial

   Mutation

   Inactivating G52E mutation

   Promoter

   T7

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDNA2.0 6H-TEV-KRAS

   Plasmid

   #111849

   Purpose

   Expresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

   Depositor

   Kenneth Westover

   Article

   Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

   Insert

   KRAS (KRAS Human)
   

   Tags

   6xHis-tag and TEV protease cleavage sequence

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. ClCry4 Y319D

    Plasmid

    #160649

    Purpose

    Expression of protein for various experiments including finding interaction partners

    Depositor

    Joseph Takahashi

    Article

    Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

    Insert

    Clcry4 PHR Y319D
    

    Tags

    Hexa his

    Expression

    Bacterial

    Available Since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETduet+p300HAT

    Plasmid

    #157793

    Purpose

    Expression of p300 HAT domain

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    p300HAT (EP300 Human)
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSETa mEos2

    Plasmid

    #20341

    Purpose

    bacterial expression of an EosFP variant, mEos2 (a photoconvertible fluorescent protein)

    Depositor

    Loren Looger

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    mEos-2
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-30a-L6KD-PT linker-PEP-Mtu ΔI-CM-xylanase

    Plasmid

    #231128

    Purpose

    Expresses xylanase via a cSAT2.0 scheme

    Depositor

    Xiaofeng Yang

    Article

    Huang et al Trends Biotechnol. 2024 Nov 12:S0167-779

    Insert

    xylanase
    

    Tags

    cSAT2.0

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. LgBiT-ST

    Plasmid

    #207633

    Purpose

    A plasmid encoding LgBiT fused to SpyTag

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    LgBiT-SpyTag
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pELxG4

    Plasmid

    #172570

    Purpose

    Inducible gene expression, pET origin, LuxR activator, PLuxB promoter, GFPmut3, Carbenicillin Resistance Marker.

    Depositor

    Christopher Reisch

    Insert

    LuxR-GFPmut3
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PLuxB

    Available Since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX6P1-DEST

    Plasmid

    #119749

    Purpose

    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag.

    Depositor

    Martin Reijns, Andrew Jackson

    Type

    Empty backbone

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21a-CysRS-His

    Plasmid

    #124107

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Cysteinyl-tRNA ligase (cysS Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST17-His10-ccdB

    Plasmid

    #166145

    Purpose

    Gateway destination vector for generation of N-terminal His10-tagged proteins in E. coli.

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Type

    Empty backbone

    Tags

    His10

    Expression

    Bacterial

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a/Cas9-Cys

    Plasmid

    #53261

    Purpose

    Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine

    Depositor

    Hyongbum Kim

    Article

    Ramakrishna et al Genome Res. 2014 Apr 2.

    Insert

    Cas9-Cys
    

    Tags

    6X His tag, HA epitope, and NLS

    Expression

    Bacterial

    Mutation

    Additional Cysteine is added to the C-terminal re…

    Promoter

    T7 Promoter

    Available Since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQmod3C-GG

    Plasmid

    #191350

    Purpose

    Clostridium expression vector (pCB102 origin, cmR) with drop-out Plac-RFP cassette flanked with BsaI sites

    Depositor

    Andrew Tolonen

    Article

    Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

    Insert

    Plac-RFP (IPTG-inducible red fluorescent protein)
    

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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