33,441 results
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Plasmid#67683PurposepMAD2 derivative designed for chromosomal gene ectopic complementation in Staphylococcus aureusDepositorTypeEmpty backboneExpressionBacterialAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only
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pCAG-Neo-PuroDTK
Plasmid#84036PurposePiggyBac cassette contains Neomycin, puromycin and TK genesDepositorTypeEmpty backboneExpressionBacterial and MammalianPromoterCAG promoterAvailable sinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDule2-3-nitroTyrosine (5B)
Plasmid#85499PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
ExpressionBacterialMutationY32H H70C D158S I159A L162RPromoterlpp (constitutive)Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPEPX-P3-sgRNAluc
Plasmid#85590PurposeIntegrate plasmid of Streptococcus pneumoniae, which can integrate sgRNA targeting luc gene, which encode luciferase, into the locus between amiF and treR. This vector can be used as the template forDepositorInsertsgRNA targeting firefly luciferase encoding gene
UseCRISPRExpressionBacterialPromoterP3Available sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTriEx-mCherry-LOV2 V416I
Plasmid#81045Purposemammalian expression of LOV V416IDepositorInsertLOV V416I
TagsmCherryExpressionBacterial, Insect, and Mamm…MutationV416IAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD-TDsmURFP-RBS-HO-1
Plasmid#80342PurposeExpresses Tandem Dimer smURFP and Heme Oxygenase-1 in bacterial cells.DepositorInsertsTandem Dimer smURFP
HO-1
Tags6 Histidine TagExpressionBacterialPromoterpBADAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUCP20T-mPlum
Plasmid#78474PurposeBroad range bacterial fluorescence labelingDepositorInsertmPlum
ExpressionBacterialAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBluescriptII[attB/Ins1]
Plasmid#74100Purpose"intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)DepositorInsertsPhi C31 attb element
Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
ExpressionBacterialAvailable sinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRSetB-Peredox
Plasmid#73806PurposeExpresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in bacteria, with N-terminal his-tagDepositorInsertPeredox
Tagshis-tag to facilitate purificationExpressionBacterialPromoterT7Available sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
Aurein1.2_(+36GFP)-Cre
Plasmid#71748PurposeAurein1.2+36GFP-Cre fusion expression in E. ColiDepositorInsert(GGS)9_Aurein1.2+36GFP-Cre
Tags6XHisExpressionBacterialAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAraH6HATT
Plasmid#63901PurposeFor expression of soluble scFv/Fab fragments in bacteria from genes selected using the pComb3H system, contains TT Fab (light and heavy chains) against tetanus toxin, used as expression control.DepositorInsertTT Fab
Tags6xHis and HA tagExpressionBacterialPromoteraraBADAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
HAI
Plasmid#71316PurposeInducible expression of human arginase I in E. coliDepositorAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Zeo-dsRedExpress
Plasmid#65040PurposeZeoR mini-Tn7 vector, mobilizable, for RFP tagging bacteriaDepositorPublicationInsertdsRedExpress
ExpressionBacterialAvailable sinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
V72 ELP
Plasmid#68938PurposeElastin-like Polypeptide (VPGxG) with 72 repeats containing Valine guest residueDepositorInsertV72 ELP
ExpressionBacterialPromoterT7Available sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCARSF63
Plasmid#64695PurposeH6-TRX-Smt3-GB1-NpuDnaEN102-CBDDepositorTypeEmpty backboneTagsCBD, HisTag, NpuDnaE-N102, Smt3, and TRXExpressionBacterialPromoterT7Available sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
MS2-Snapf
Plasmid#65860PurposeExpresses RNA-binding protein MS2 with fast SNAP fusion tagDepositorInsertsMS2
Fast SNAP
TagsHA and NLSExpressionBacterial and YeastAvailable sinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-UBE2S-UBD
Plasmid#66713PurposeExpresses the UBE2S-UBD fusion protein in E. coliDepositorAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYTK072
Plasmid#65179PurposeEncodes ConRE as a Type 5 part to be used in the Dueber YTK systemDepositorPublicationInsertConRE
ExpressionBacterialAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-iLOVf
Plasmid#63723PurposeE coli expression plasmid to produce Thrombin cleavable His tagged iLOV fusion proteinsDepositorTypeEmpty backboneTagsTEE, iLOV, 6xHis, thrombin cleavage siteExpressionBacterialPromoterT7Available sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pACYC-LIC+
Plasmid#62312PurposeE. coli pACYC based expression vector with N-terminal Hexa-HIS and a thrombin protease cleavage siteDepositorTypeEmpty backboneTags6xHis and thrombin protease cleavage siteExpressionBacterialPromoterT7-lacOAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by