30,743 results

1. p3998 pGEX HHR23A

   Plasmid

   #10864

   Depositor

   Peter Howley

   Article

   Kumar et al J Biol Chem. 1999 Jun 25. 274(26):18785-

   Insert

   HHR23A (RAD23A Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pSWAP_Entry_H1_alpha

   Plasmid

   #184865

   Purpose

   pSwap plasmid part with H1_alpha homology arms

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   lacZalpha,ccdB
   

   Expression

   Bacterial

   Available Since

   Jan. 3, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. strep-IRF8

   Plasmid

   #102872

   Purpose

   Express strep-tagged mouse IRF8 protein in E. coli

   Depositor

   Gary Stormo

   Article

   Chang et al BMC Mol Biol. 2018 Mar 27;19(1):5. doi:

   Insert

   IRF8 (Irf8 Mouse)
   

   Tags

   strep tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSET-RCaMP1h

   Plasmid

   #42874

   Purpose

   a single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRuby

   Depositor

   Loren Looger

   Article

   Akerboom et al Front Mol Neurosci. 2013;6:2. doi: 10.33

   Insert

   Red Fluorescent Calcium binding Protein
   

   Tags

   EK (Enterokinase) Recognition Site, His Tag (6x),…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. TOPO mKate2

   Plasmid

   #68441

   Purpose

   TOPO construct expressing mKate2 for generation of GMAP-compatible gene

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   mKate2
   

   Use

   Gmap

   Expression

   Bacterial

   Promoter

   Plac

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pASKt15C+SrtA7

   Plasmid

   #64986

   Purpose

   Expresses the P94R/E105K/E108A/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

   Insert

   sortase A
   

   Tags

   His6 tag and S tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59, P94R, E105K, E108A, D16…

   Promoter

   tetracycline promoter/operator

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBFC1171

   Plasmid

   #231994

   Purpose

   CRISPRi-ART crRNA only plasmid encoding crystal violet-inducible crRNA (for dRfxCas13d) with 2xBsaI spacer cloning site

   Depositor

   Brady Cress

   Article

   Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d

   Insert

   crRNA of dRfxCas13d
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   pJEx (Jungle Express)

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAT218

   Plasmid

   #134886

   Purpose

   Bacterial expression

   Depositor

   Thomas Howard

   Article

   Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-29b(+)_NSP4ss_SerM_NucA-myc

   Plasmid

   #218191

   Purpose

   Bacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)

   Depositor

   Florence Pojer

   Insert

   nucA
   

   Tags

   NSP4 signal secretion peptide and myc

   Expression

   Bacterial

   Mutation

   codon optimized ecoli residues - 22-266

   Promoter

   T7

   Available Since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-PKM2-WT

    Plasmid

    #122690

    Purpose

    Express His-tagged PKM2 (wild type) protein in bacteria.

    Depositor

    Wen-Ching Wang

    Article

    Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

    Insert

    Pyruvate kinase M2 isoform (PKM Human)
    

    Tags

    6His-tag

    Expression

    Bacterial

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBTK503

    Plasmid

    #110616

    Purpose

    BTK assembled broad-host-range plasmid encoding GFP driven by CP25 promoter

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Expression

    Bacterial

    Available Since

    Aug. 10, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pMBP-hLIF

    Plasmid

    #179711

    Purpose

    Expresses H6-MBP-LIF

    Depositor

    Mark Arbing

    Insert

    Leukemia inhibitory factor (LIF Human)
    

    Tags

    His6-MBP-TEV protease site

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXW109Hg(RS-RinA-E11)2

    Plasmid

    #123151

    Purpose

    Mercury sensor with three-layered amplifier cascade. Need to work with plasmid pXWP11-gfp2. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J109, PmerT, PhrpLE, PrinA

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. mt POLRMT

    Plasmid

    #205059

    Purpose

    POLRMT protein expression

    Depositor

    Smita Patel

    Article

    Ramachandran et al Nucleic Acids Res. 2017 Jan 25;45(2):861

    Insert

    POLRMT (POLRMT Human)
    

    Expression

    Bacterial

    Mutation

    Codon optimized

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET11a_3xFlag-nsp14 (SARS-CoV-2)

    Plasmid

    #169159

    Purpose

    To express SARS-CoV-2 nsp14 in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    3xFlag-nsp5CS[VATLQ]-nsp14 (ORF1ab SARS-CoV-2)
    

    Tags

    3xFlag and nsp5CS[VATLQ]: predicted nsp5 protease…

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T7

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. plYJP022-int7

    Plasmid

    #202287

    Purpose

    Plasmid containing integrase for LPattB7 site

    Depositor

    Christopher Voigt

    Article

    Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

    Insert

    int7
    

    Expression

    Bacterial

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX Grb2 SH2-SH3

    Plasmid

    #46442

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Grb2 (GRB2 Human)
    

    Tags

    GST and Thrombin site

    Expression

    Bacterial

    Mutation

    contains amino acids 1-217

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSF-KpAGO207-1251

    Plasmid

    #117854

    Purpose

    Expressed KpAGO 207-1251 in E. coli under IPTG-inducible promotor

    Depositor

    Dinshaw Patel, Kotaro Nakanishi

    Article

    Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

    Insert

    Argonaute
    

    Tags

    6xHis-SUMO encoded in vector

    Expression

    Bacterial

    Mutation

    missing first 206 amino acid residues

    Promoter

    T7

    Available Since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a T7pCONS TIR-1 sfGFP

    Plasmid

    #154463

    Purpose

    pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

    Depositor

    Daniel Daley

    Article

    Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

    Insert

    super folder GFP
    

    Tags

    His-thrombin

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-TRSter

    Plasmid

    #78865

    Purpose

    Expresses rat Thioredoxin Reductase using an open reading frame in fusion with a mutated E. coli-type SECIS element

    Depositor

    Elias Arnér

    Article

    Arner et al J Mol Biol. 1999 Oct 8;292(5):1003-16.

    Insert

    Thioredoxin Reductase (Txnrd1 Rat)
    

    Tags

    Mutated E. coli-type SECIS element (3’ end of ins…

    Expression

    Bacterial

    Promoter

    T7lac

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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