30,743 results

1. pCIRCgp41-1

   Plasmid

   #74227

   Purpose

   Vector for SICLOPPS-mediated protein circularization using the gp41-1 intein.

   Depositor

   Roland Eils, Barbara Di Ventura

   Article

   Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   gp41-1 C-intein and gp41-1 N-intein

   Expression

   Bacterial

   Promoter

   T7-lac

   Available Since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNBU2_repA3

   Plasmid

   #211912

   Purpose

   Replicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.

   Depositor

   Adam Arkin

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Feb. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJ4M_hnRNPA2_FL_WT

   Plasmid

   #139109

   Purpose

   Express FL hnRNPA2 with a C-terminal MBP tag

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

   Insert

   hnRNPA2 (HNRNPA2B1 Human)
   

   Expression

   Bacterial

   Mutation

   none

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCyPet-His

   Plasmid

   #14030

   Depositor

   Patrick Daugherty

   Article

   Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

   Insert

   CyPet
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Feb. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB Neo* PB

   Plasmid

   #138413

   Purpose

   Minimal piggyBac ITR-flanked pPgk-pEM7-Neo*-bpA positive selection cassette

   Depositor

   Mario Capecchi

   Insert

   Neo*
   

   Use

   Mouse Targeting; Piggybac

   Expression

   Bacterial and Mammalian

   Available Since

   Nov. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJMP1159

   Plasmid

   #119250

   Purpose

   rfp "test" strain

   Depositor

   Oren Rosenberg, Carol Gross, Jason Peters

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   sgRNA NT1/RR1 (rfp)
   

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBEL1092

   Plasmid

   #195635

   Purpose

   Expression/purification of 6xHis-TEV-sfGFP

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

   Insert

   sfGFP
   

   Expression

   Bacterial

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSP1E2

   Plasmid

   #20063

   Depositor

   Stephen Sligar

   Article

   Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

   Insert

   MSP1E2 (APOA1 synthetic gene)
   

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   "extended" MSP1; contains repeats of he…

   Available Since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRAM18dRGA[MCS]

   Plasmid

   #84683

   Purpose

   Can be used for the transformation of rickettsia

   Depositor

   Ulrike Munderloh

   Article

   Burkhardt et al PLoS One. 2011;6(12):e29511. doi: 10.137

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pComb3XLambda

    Plasmid

    #63892

    Purpose

    Backbone pComb3X containing PCR template for human antibody lambda light chain constant region

    Depositor

    Carlos Barbas

    Article

    Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

    Insert

    human antibody lambda light chain (IGLL5 Human)
    

    Use

    Phage display

    Expression

    Bacterial

    Promoter

    lacZ

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas9cur

    Plasmid

    #71538

    Purpose

    Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

    Depositor

    Tao Chen

    Article

    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

    Inserts

    Cas9
    Plasmid curing system
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-16b-10His-TEV-MCES_VACCA_D1_D12

    Plasmid

    #203671

    Purpose

    The D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavage

    Depositor

    Stephen Cusack

    Article

    Kyrieleis et al Structure. 2014 Mar 4;22(3):452-65. doi:

    Insert

    MCES_VACCA_D1_D12
    

    Tags

    10xHis

    Expression

    Bacterial

    Available Since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMesh1*

    Plasmid

    #175591

    Purpose

    Lac inducible, AMP Resistant, fluor labelled (CFP) gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.

    Depositor

    Greg Bokinsky

    Article

    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

    Insert

    Mesh1 (Mesh1 Fly)
    

    Tags

    Fused with a flexible GS linker to cerulean

    Expression

    Bacterial

    Mutation

    Codon optimized for translation in E.coli. AGGAGG…

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHA2

    Plasmid

    #85710

    Purpose

    This plasmid provides a BAC vector backbone containing GFP, which can be used for cloning of BAC inserts, for instance, herpesvirus genomes

    Depositor

    Martin Messerle, Ulrich Koszinowski

    Article

    Adler et al J Virol. 2000 Aug;74(15):6964-74.

    Type

    Empty backbone

    Use

    Cre/Lox; Herpesvirus cloning

    Expression

    Bacterial

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPSU30

    Plasmid

    #173830

    Purpose

    Expresses 30 kD Penn State ladder protein

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    HSTPABPACS100BCBP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPSU10

    Plasmid

    #173827

    Purpose

    Expresses 10 kD Penn State ladder protein

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    STRHSTPAB
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOSIP-TH (TetR, HK022)

    Plasmid

    #45988

    Depositor

    Keith Shearwin, Drew Endy

    Article

    St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBSV2_P0826-mCherryBb

    Plasmid

    #118242

    Purpose

    Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0826 promoter driven mCherry

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Insert

    bb0826 promoter driven mCherry
    

    Expression

    Bacterial

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. PBAD-his6-prkA-pACYC184

    Plasmid

    #41041

    Depositor

    Ichiro Matsumura

    Article

    Parikh et al Protein Eng Des Sel. 2006 Mar;19(3):113-

    Insert

    prkA
    

    Tags

    6xHis and T7 epitope tag

    Expression

    Bacterial

    Promoter

    PBAD

    Available Since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET21b-Caspase-3

    Plasmid

    #90087

    Purpose

    Expresses wild type caspase 3 in bacterial cells

    Depositor

    Clay Clark

    Article

    Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

    Insert

    Caspase 3 (CASP3 Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Wild type

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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