32,693 results
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Plasmid#206208PurposeContains two plasmids. Constitutive expression of BsmAIM and inducible expression of BsaIRDepositorInsertBsamAIM and BsaIR
ExpressionBacterialAvailable SinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-Myc
Plasmid#119757PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Myc tag.DepositorTypeEmpty backboneTagsGST and MycExpressionBacterialPromotertac promoterAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pS448∙CsR
Plasmid#122096PurposeCRISPR/Cas9 counterselection in Gram-negative bacteriaDepositorInsertsCas9
sgRNA
UseSynthetic BiologyExpressionBacterialPromoterPem7 and PmAvailable SinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQFT-mNG
Plasmid#203675PurposepQFT with mNeonGreen under control of PQJDepositorInsertmNeonGreen
ExpressionBacterialPromoterPQJAvailable SinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDonor14-N7HELIX-KanR
Plasmid#190663PurposeKanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequenceDepositorInsertKanR, R6K origin of replication
ExpressionBacterialPromoterNAAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p1-GST-Rnf31
Plasmid#63134PurposeExpression of human RNF31/HOIP codon optimized for E. coli and insect cellsDepositorInsertRnf31 (RNF31 Synthetic, Human)
TagsGSTExpressionBacterialMutationSynthetic based on human protein sequencePromotertacAvailable SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFPN1-human Dynamin 1aa
Plasmid#22163DepositorAvailable SinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNP566
Plasmid#222724PurposepNP561 - IS621 WT Target with T7 Inducible IS621 RecombinaseDepositorInsertIS621 Recombinase
ExpressionBacterialAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS316-RGR-GFP
Plasmid#51056PurposeExpress self-cleaving-ribozyme-flanked sgRNA cassette (RGR) targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'.DepositorInsertRGR-GFP
UseCRISPRExpressionBacterial and YeastPromoterYeast ADH1 promoterAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBAD/His-miRFP670nano
Plasmid#127427PurposeMonomeric near-infrared fluorescent protein miRFP670nano in bacterial plasmidDepositorInsertmiRFP670nano
TagsHisExpressionBacterialPromoteraraCAvailable SinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTW211b5
Plasmid#136927PurposeDirected evolution of SpCas9DepositorInsertCas9(N)-NpuN
UseCRISPRExpressionBacterialMutationsee manuscriptAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSELIS-RamR
Plasmid#202631PurposeEnables dual growth and fluorescence based enrichment of RamR biosensorsDepositorInsertSh ble
UseSynthetic BiologyExpressionBacterialPromoterLambda promoterAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSiMPlc_N + pSiMPlc_C
Plasmid#134312PurposeMaintenance of 2 plasmids with a single antibiotic in E. coliDepositorInsertcmR_gp41-1 N-intein + cmR_gp41-1 C-intein
ExpressionBacterialAvailable SinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET11a-link-NGFP
Plasmid#52732PurposeIPTG-inducible expression of link-NGFP negative control for in vivo split GFP assembly assayDepositorInsertlink-NGFP
TagsHis tagExpressionBacterialPromoterT7Available SinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTW358f
Plasmid#227935PurposeExpress hIAPP-T7nDepositorInserthIAPP-T7n
ExpressionBacterialAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET.HaloTag.v7
Plasmid#226197Purposebacterial expression plasmid for HaloTag v7, N-terminus 6xHIS tag for affinity purificationDepositorInsertHaloTag.v7
Tags6xHISExpressionBacterialPromoterT7Available SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
BRD4
Plasmid#38942PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pETMCSI-T4P_Y320A
Plasmid#122522PurposeExpression in BL21(DE3) PlysS of mutant T4 DNA polymerase Y320A for easy cloning and mutagenesisDepositorInsertgp43 - DNA polymerase (43 Escherichia virus T4)
ExpressionBacterialMutationtyrosine 320 changed to alaninePromoterT7 promoterAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUCP20T-E2Crimson
Plasmid#78473PurposeBroad range bacterial fluorescence labelingDepositorInsertE2-Crimson
ExpressionBacterialAvailable SinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD-Citroff1
Plasmid#134301PurposeBacterial expression of inverse-response citrate sensor with high binding affinityDepositorInsertCitroff1
Tags6-His tagExpressionBacterialPromoterpBADAvailable SinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only