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GST-PRMT9 WT

Plasmid

#67607

Purpose

bacterial expression of wild type human PRMT9

Depositor

Steven Clarke

Publication

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Tags

GST

Expression

Bacterial

Mutation

wild type

Promoter

tac

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pZ8-P_dCas9

Plasmid

#74063

Purpose

pZ8-1 plasmid carrying dcas9 driven by the propionate-inducible prpD2 promoter (PprpD2), KanR

Depositor

Timothy Lu

Publication

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

dcas9

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Propionate inducible promoter (prp)

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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Reb_1 aka PAS619

Plasmid

#71224

Purpose

Expresses wild type R bodies from C. taeniospiralis in pETM11 backbone

Depositor

Pamela Silver

Publication

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Tags

none

Expression

Bacterial

Mutation

RebA G53A

Promoter

T7

Available since

April 7, 2016

Availability

Academic Institutions and Nonprofits only
pBSJL1

Plasmid

#71261

Purpose

E. coli-Veillonella shuttle plasmid

Depositor

Felicia Qi

Publication

Liu et al Appl Environ Microbiol. 2012 May;78(9):3

Type

Empty backbone

Use

E. coli-veillonella shuttle plasmid

Expression

Bacterial

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only
pMAL-c5X-ELP[V-60]

Plasmid

#67013

Purpose

Expresses malE-ELP[V-60] in E. coli

Depositor

Ashutosh Chilkoti

Publication

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-ELP[V-60]

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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I48 ELP

Plasmid

#68937

Purpose

Elastin-like polypeptide (VPGxG) with 48 repeats of Isoleucine guest residue

Depositor

Andrew MacKay

Publication

Shi et al J Control Release. 2013 Nov 10;171(3):33

Insert

I48 ELP

Expression

Bacterial

Promoter

T7

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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CYP2C9 in vector pCW ori+

Plasmid

#69554

Purpose

Modification of CYP2C9 and 2C19 s for bacterial expression

Depositor

Joyce Goldstein

Insert

CYP2C9 (CYP2C9 Human)

Expression

Bacterial

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSNAP-CLC-EYFP

Plasmid

#38011

Depositor

Xiaowei Zhuang

Publication

Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

Insert

Clathrin, light polypeptide (LCa) (Clta Mouse)

Tags

EYFP and SNAP26m

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Sept. 29, 2015

Availability

Academic Institutions and Nonprofits only

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MP2

Plasmid

#69628

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Publication

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926

Expression

Bacterial

Promoter

E. coli PBAD

Available since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pBSK

Plasmid

#67504

Purpose

Express the tRNALys scaffold

Depositor

Luc Ponchon

Publication

Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

Insert

modified tRNALys human

Expression

Bacterial

Promoter

lpp

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pUC18T-mini-Tn7T-Tp-eyfp

Plasmid

#65035

Purpose

TpR mini-Tn7 vector, mobilizable, for YFP tagging bacteria

Depositor

Herbert Schweizer

Publication

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

eyfp

Expression

Bacterial

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pJKR-O-mphR

Plasmid

#62570

Purpose

Macrolide biosensor. Medium copy plasmid that expresses GFP proportional to macrolide concentration. Compatible with all other pJKR plasmids.

Depositor

George Church

Publication

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

mCherry

Expression

Bacterial

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_Nkx6.2

Plasmid

#64846

Purpose

A lentiviral vector for the expression of mouse Nkx6.2 under doxycyline control

Depositor

Paul Tesar

Publication

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Nkx6.2 (Nkx6-2 Mouse)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBAD_GFP11_T7LysH17A

Plasmid

#59591

Purpose

Expression vector for solubility screening of proteins or protein fragments using a split-GFP assay.

Depositor

Richard Kingston

Publication

Bulloch et al Anal Biochem. 2014 Jul 10. pii: S0003-26

Type

Empty backbone

Tags

6xHis-tag and GFP11

Expression

Bacterial

Promoter

pARA

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD1 (OTU+UIM, aa 287-481)

Plasmid

#61406

Purpose

Expresses human OTUD1 (OTU+UIM domains) in E. coli.

Depositor

David Komander

Publication

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD1 (OTUD1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-286.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pIntegrase_8

Plasmid

#60579

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Publication

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_8

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pTRC-LIC

Plasmid

#62343

Purpose

Used for tac promoter regulated high-level expression of cloned, untagged genes in E. Coli.

Depositor

Cheryl Arrowsmith

Type

Empty backbone

Expression

Bacterial

Promoter

tac promoter from pKK233-2

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pPEP1

Plasmid

#61046

Purpose

BglFusion compatible integration vector for Streptococcus pneumoniae

Depositor

Jan-Willem Veening

Publication

Sorg et al ACS Synth Biol. 2014 Jun 4.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTE105

Plasmid

#59400

Purpose

MethyloBrick expression vector, tuf promoter; TcR

Depositor

Tobias Erb

Publication

Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptuf

Available since

Dec. 30, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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DJ-1 pET15b

Plasmid

#60678

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Publication

Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

Insert

DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only