34,200 results
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Plasmid#64846PurposeA lentiviral vector for the expression of mouse Nkx6.2 under doxycyline controlDepositorAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pBAD_GFP11_T7LysH17A
Plasmid#59591PurposeExpression vector for solubility screening of proteins or protein fragments using a split-GFP assay.DepositorTypeEmpty backboneTags6xHis-tag and GFP11ExpressionBacterialPromoterpARAAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD1 (OTU+UIM, aa 287-481)
Plasmid#61406PurposeExpresses human OTUD1 (OTU+UIM domains) in E. coli.DepositorInsertOTUD1 (OTUD1 Human)
TagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-286.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pIntegrase_8
Plasmid#60579Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_8
ExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTRC-LIC
Plasmid#62343PurposeUsed for tac promoter regulated high-level expression of cloned, untagged genes in E. Coli.DepositorTypeEmpty backboneExpressionBacterialPromotertac promoter from pKK233-2Available sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPEP1
Plasmid#61046PurposeBglFusion compatible integration vector for Streptococcus pneumoniaeDepositorPublicationTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTE105
Plasmid#59400PurposeMethyloBrick expression vector, tuf promoter; TcRDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPtufAvailable sinceDec. 30, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTSara
Plasmid#60720PurposeExpresses both fragments of T7 RNAP split at position 179. Both fragments are driven by the arabinose inducible promoter PBAD. Also contains a constitutive araC ORFDepositorInsertsN Terminal fragment of T7 RNAP split betwen 179-180
N Terminal fragment of T7 RNAP split betwen 179-180
ExpressionBacterialMutationT7 RNAP split between positions 179-180 and wt T7…PromoterPBADAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10)
Plasmid#55209DepositorTypeEmpty backboneTags10xAsn, 10xHis, MPB, and TEV cleavage siteExpressionBacterialPromoterT7Available sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET StrepII-StrepII TEV LIC cloning vector (2RRT)
Plasmid#55215DepositorTypeEmpty backboneTags2xStrep II and TEV cleavage siteExpressionBacterialPromoterT7Available sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCCL-ReDNAP (Y427A)
Plasmid#52346PurposeCEN6/ARS4 plasmid, REV1 promoter>Recoded TP-DNAP1 (Y427A)DepositorInsertRecoded TP-DNAP
ExpressionBacterial and YeastPromoterREV1Available sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-HA-ERK2 K54R
Plasmid#53170PurposeExpresses GST-HA tagged human ERK2 K54R from bacterial expression vectorDepositorAvailable sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pXylA-agrCA-I
Plasmid#53437Purposexylose-dependent expression of the type-I agrC and agrA genes of S. aureusDepositorInsertagrCA locus
UseSynthetic BiologyTagsnoneExpressionBacterialMutation"MVQTS" sequence added to the N-terminu…PromoterPxylAAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSM3
Plasmid#49799PurposeePathBrick vector for pathway engineering in E. coli.DepositorTypeEmpty backboneTagsS-tagExpressionBacterialPromoterT7Available sinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRa20_992
Plasmid#49673DepositorInsertECF20_992
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-HIS-Sangamo
Plasmid#40786Purposefor e. coli expression, Golden Gate Compatible TALEN incorporated with N Terminal 6xHIS Tag, Codon Optimized FokIDepositorInsertTranscription Activator Like Effector Nuclease
UseTALENTagsN Terminal 6xHISExpressionBacterialMutation+63 C Terminus, Codon Optimized FokI EndonucleasePromoterT7Available sinceDec. 12, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTD-C_sfYFPTwinStrep
Plasmid#45943Purposeexpresses P.putida codon optimized super-folder sfYFP-Twin-Strep fusion proteinDepositorInsertsynthetic sfYFP (codon usage adapted to P.putida KT2440)
TagsTwin-Strep-tagExpressionBacterialPromoterlacIq/PtrcAvailable sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAraGFPCDF
Plasmid#47516PurposeContains PBAD::GFP reporter, used for heterodimer competition assayDepositorInsertGFP
ExpressionBacterialAvailable sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWA PAS-E BAD K-GAFm
Plasmid#39866DepositorInsertsiRFP protein PAS domain
iRFP protein GAF domain (mutated)
TagsE coil and K coilExpressionBacterialMutationF165Y, D232Y, W308RPromoterarabinose inducible and rhamnose inducibleAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZE-PalkB-GFP
Plasmid#44381DepositorInsertsGFP
PalkB
UseSynthetic BiologyExpressionBacterialPromoterPalkBAvailable sinceApril 17, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits