30,744 results

1. pQFT-mNG

   Plasmid

   #203675

   Purpose

   pQFT with mNeonGreen under control of PQJ

   Depositor

   Urs Jenal

   Article

   Klotz et al Appl Environ Microbiol. 2023 May 18:e002

   Insert

   mNeonGreen
   

   Expression

   Bacterial

   Promoter

   PQJ

   Available Since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PVS10

   Plasmid

   #104398

   Purpose

   polycistronic vector for expression of E. coli core RNAP

   Depositor

   Irina Artsimovitch

   Article

   Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

   Insert

   rpoA-rpoB-rpoC-6xHis and rpoZ (rpoA, rpoB, rpoC, rpoZ E. coli)
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZP06B

   Plasmid

   #232321

   Purpose

   Make sacB a counterselection marker to replace galK

   Depositor

   Chenggang Wu

   Insert

   PrpsJ-sacB
   

   Expression

   Bacterial

   Available Since

   March 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAM5409

   Plasmid

   #132662

   Purpose

   RSF1010-based broad host-range plasmid with RK2 bom site expressing YFP and Sp/Sm resistance

   Depositor

   Susan Golden

   Article

   Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

   Inserts

   aadA
   YFP
   

   Expression

   Bacterial

   Promoter

   PaadA and PconII

   Available Since

   Nov. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSET-B mCherry

   Plasmid

   #108857

   Purpose

   Encodes for the fluorescent protein mCherry

   Depositor

   Kalina Hristova

   Article

   Sarabipour et al Anal Biochem. 2014 Mar 15;449:155-7. doi

   Insert

   mCherry
   

   Expression

   Bacterial

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMLS7-egfp

   Plasmid

   #112917

   Purpose

   Fluorescence microscopy

   Depositor

   Miguel Valvano

   Article

   Lefebre et al Appl Environ Microbiol. 2002 Dec;68(12):

   Insert

   eGFP
   

   Expression

   Bacterial

   Promoter

   PS7 promoter and Ps7

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. His-FGE

   Plasmid

   #75147

   Purpose

   Expression vector for His(6) tagged Formylglycine Generating Enzyme (FGE) in bacterial host.

   Depositor

   Richard Fishel

   Article

   Liu et al Sci Rep. 2015 Nov 19;5:16883. doi: 10.10

   Insert

   FGE
   

   Tags

   Histidine tag and Thrombin

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   T7

   Available Since

   July 8, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pBR322-Pdp1_NTD-GFP-HiBiT

   Plasmid

   #198317

   Purpose

   Loads HiBiT-tagged GFP into PVCs using Pdp1 packaging domain; also contains Pnf

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   Pdp1_NTD-GFP-HiBiT
   

   Expression

   Bacterial

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEM-Cas9HF1-recA56

   Plasmid

   #89962

   Purpose

   Modified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Inserts

   Cas9HF1
   recA56
   

   Expression

   Bacterial

   Mutation

   N497A/R661A/Q695A/Q926A

   Promoter

   pTet and proD

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. PTP1B human

    Plasmid

    #102719

    Purpose

    human PTP1B catalytic domain (residues 1-301) in RP1B vector

    Depositor

    Wolfgang Peti

    Article

    Choy et al Mol Cell. 2017 Feb 16;65(4):644-658.e5.

    Insert

    Protein Tyrosine Phosphatase 1B (PTP1B) (PTPN1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFC330

    Plasmid

    #87842

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and pyrG selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    pyrG
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Promoter

    Aspergillus nidulans tef1 promoter and native pro…

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOPINM

    Plasmid

    #26044

    Depositor

    Ray Owens

    Article

    Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

    Insert

    His-MBP-3C-lacZ
    

    Tags

    MAHHHHHHSSG_MBP_LEVLFQGP

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOPINB

    Plasmid

    #41142

    Depositor

    Ray Owens

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    March 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28CLY1

    Plasmid

    #21760

    Depositor

    Maarten Merkx

    Article

    Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

    Insert

    ECFP -EYFP linked by flexible one SGGSGG repeats
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Dec. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. GST-TACC3-His6

    Plasmid

    #69106

    Purpose

    For bacterial expression of recombinant human TACC3 tagged with GST and His.

    Depositor

    Stephen Royle

    Article

    Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

    Insert

    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    

    Tags

    GST and His6

    Expression

    Bacterial

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pProEx Rac1(G12C) CL

    Plasmid

    #224269

    Purpose

    Protein purification of Rac1 (G12C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    Rac1 (RAC1 Human)
    

    Expression

    Bacterial

    Mutation

    G12C

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETDuet-1∆HisTag_hNMT_Nef

    Plasmid

    #66078

    Purpose

    Single vector system expressing hNMT1 (81-496 amino acids) and NEF allowing myristoylation of recombinant protein produce in Ecoli system

    Depositor

    Dieter Willbold

    Article

    Gluck et al PLoS One. 2010 Apr 9;5(4):e10081. doi: 1

    Inserts

    NMT (NMT1 Human)
    Nef
    

    Expression

    Bacterial

    Mutation

    Deletion of first 80 amino acids

    Promoter

    T7-lacO

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAWP78-PVCpnf_pvc13-antiMouseMHCIINb

    Plasmid

    #198292

    Purpose

    Produces PVCs retargeted with VHH7 anti-mouse MHC class II nanobody

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc13-antiMouseMHCIINb
    

    Expression

    Bacterial

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pK27Sumo_His-SUMO-nsp12 (SARS-CoV-2)

    Plasmid

    #169188

    Purpose

    To express His-SUMO-nsp12 (SARS-CoV-2) in E. coli

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    14His-SUMO-nsp12 (ORF1ab SARS-CoV-2, Synthetic)
    

    Tags

    14His-SUMO (Ulp1-cleavable)

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHybrid-Electra1

    Plasmid

    #179474

    Purpose

    Expresses Electra1 in bacterial cells and Electra1 and mScarlet in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Inserts

    Electra1
    mScarlet-I
    

    Expression

    Bacterial and Mammalian

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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