29,729 results

1. AP1

   Plasmid

   #179678

   Purpose

   Accessory plasmids

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   [SD8] gIII
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a_SYK

   Plasmid

   #224240

   Purpose

   Expression of human SYK Kinase in E. coli

   Depositor

   Andrew Mesecar

   Insert

   syk (SYK Human)
   

   Use

   Tags

   6xHis and 6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promotor

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a_LYN

   Plasmid

   #224238

   Purpose

   Expression of human LYN Kinase in E. coli

   Depositor

   Andrew Mesecar

   Insert

   lyn (LYN Human)
   

   Use

   Tags

   6xHis and 6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promotor

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNHA5

   Plasmid

   #223647

   Purpose

   TurboID protein purification

   Depositor

   François-Xavier Campbell-Valois

   Article

   Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

   Insert

   TurboID
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   Promoter

   LacI

   Available since

   Oct. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pNHA6

   Plasmid

   #223648

   Purpose

   BioID protein purification

   Depositor

   François-Xavier Campbell-Valois

   Article

   Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

   Insert

   BioID
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   Promoter

   LacI

   Available since

   Oct. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pNHA7

   Plasmid

   #223649

   Purpose

   birA protein purification

   Depositor

   François-Xavier Campbell-Valois

   Article

   Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

   Insert

   BioID_G118R
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   G118R

   Promoter

   LacI

   Available since

   Oct. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pSR1058

   Plasmid

   #208743

   Purpose

   Characterization plasmid for PHbs (sfgfp expressed from PHbs on the S. aureus pC194 replicon)

   Depositor

   Lauren Andrews

   Article

   Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

   Insert

   PHbs
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PHbs

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETM-60-NusA-PfAgo_F

   Plasmid

   #146270

   Purpose

   Bacterial Expression of PfAgo

   Depositor

   Elisa Izaurralde

   Insert

   PfAgo
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   one non silent mutation A907G (I303V) and one sil…

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAD29_hIRG1_4-461_pvp008

   Plasmid

   #124843

   Purpose

   Expresses human CAD (Irg1) in E.coli

   Depositor

   Konrad Buessow

   Article

   Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

   Insert

   cis-aconitate decarboxylase (ACOD1 Human)
   

   Use

   Tags

   StrepTag and TEV site

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-3 and 462-end.

   Promoter

   T7

   Available since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSFDuet-1_VNp-His6

    Plasmid

    #182386

    Purpose

    Bacterial expression of Vesicle Nucleating peptide

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-His
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBbA5k-acrAB-sfGFP

    Plasmid

    #195516

    Purpose

    Inducible expression vector for transcriptionally fused acrAB and sfGFP.

    Depositor

    Mary Dunlop

    Article

    Wen et al Sci Rep. 2018 Oct 11;8(1):15120. doi: 10

    Insert

    acrAB
    

    Use

    Synthetic Biology

    Tags

    sfgfp

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. PYCR1

    Plasmid

    #38842

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PYCR1 (PYCR1 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pMAL-XPG

    Plasmid

    #39215

    Depositor

    Aziz Sancar

    Article

    Matsunaga et al J Biol Chem. 1995 Sep 1;270(35):20862-9.

    Insert

    XPG (ERCC5 Human)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Note that XPG starts at amino acid 5

    Promoter

    Ptac

    Available since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJBEI-6410

    Plasmid

    #47049

    Purpose

    BglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli

    Depositor

    Taek Soon Lee

    Article

    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

    Insert

    Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
    

    Use

    Synthetic Biology; Bglbrick

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacUV5

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-HisYFP-Sp100

    Plasmid

    #53141

    Purpose

    E.coli expression of HisYFP tagged human Sp100 fragment

    Depositor

    Frauke Melchior

    Article

    Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

    Insert

    Sp100A fragment (SP100 Human)
    

    Use

    Tags

    His-YFP

    Expression

    Bacterial

    Mutation

    fragment (amino acids 71-480); deletion of L75

    Promoter

    T7

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis

    Plasmid

    #62373

    Purpose

    Expression of (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
    

    Use

    Tags

    (-30)dGFP, 6xHis tag, Cathepsin cleavage site, an…

    Expression

    Bacterial

    Mutation

    Y67S amino acid substitution in (-30)GFP renders …

    Promoter

    T7

    Available since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHN764

    Plasmid

    #178819

    Purpose

    Expression of EndoH in E. coli

    Depositor

    Kazuhiro Abe

    Insert

    synthetic gene encoding endo-β-N-acetylglucosaminidase H
    

    Use

    Tags

    HIs8 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSET-4xTR-TUBE

    Plasmid

    #110312

    Purpose

    E. coli expression and purification of 4xTR-TUBE

    Depositor

    Yasushi Saeki

    Article

    Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

    Insert

    4xTR-TUBE (UBQLN1 Human)
    

    Use

    Tags

    N-terminal His6-T7 tag

    Expression

    Bacterial

    Mutation

    All Arg residues in the UBA domain are mutated to…

    Promoter

    T7

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET15b CnA CnB

    Plasmid

    #11787

    Depositor

    Anjana Rao

    Article

    Mondragon et al Biochemistry. 1997 Apr 22. 36(16):4934-4

    Insert

    CnA CnB (PPP3R1, PPP3CA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 4, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pET28-5'TwStrep

    Plasmid

    #208620

    Purpose

    Empty vector for prokaryotic expression, insert a Twin Strep tag between NdeI and BamHI site of pET28a (+), both site retained, T7 tag replaced, BamHI site in-frame with Twin Strep tag.

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Use

    Tags

    Twin Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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