30,744 results

1. pET22b-PelB-6H-Ag43-160N-sfGFP

   Plasmid

   #172886

   Purpose

   Display sfGFP protein on E. coli cell surface

   Depositor

   Urartu Seker

   Article

   Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

   Inserts

   SfGFP
   Ag43, partial (flu )
   

   Tags

   HHHHHH and TEV recognition site (ENLYFQG)

   Expression

   Bacterial

   Mutation

   Deleted 1-160 (N terminal)

   Promoter

   T7 Promoter

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSB223

   Plasmid

   #164127

   Purpose

   New measurement standard

   Depositor

   Christopher Voigt

   Article

   Shao et al Nat Commun. 2021 Mar 5;12(1):1475. doi:

   Insert

   YFP-PP7
   

   Expression

   Bacterial

   Promoter

   J23101

   Available Since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAJM.771

   Plasmid

   #108534

   Purpose

   NahRAM +PSalTTC-YFP Reporter. Sal sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   NahRAM + PSalTTC-YFP
   

   Expression

   Bacterial

   Mutation

   NahR: I25V P72H V118A R248C

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. 6His-MBP-TEV-huLbCpf1

   Plasmid

   #90096

   Purpose

   Bacterial expression plasmid for protein purification

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Insert

   huLbCpf1
   

   Tags

   3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

   Expression

   Bacterial

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21-pelB-LaMIgG-14

   Plasmid

   #228994

   Purpose

   For bacterial expression of anti-mouse IgG nanobody LaMIgG-14, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaMIgG-14
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21-pelB-LaTdT-39

   Plasmid

   #228980

   Purpose

   For bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaTdT-39
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBL090J

   Plasmid

   #195045

   Purpose

   cCadC-CCB3.5 under the control of PBAD

   Depositor

   Tina Wang

   Article

   Lee et al ACS Synth Biol. 2022 Oct 21;11(10):3198-

   Insert

   cCadC-CCB3.5
   

   Expression

   Bacterial

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOPINE

   Plasmid

   #26043

   Depositor

   Ray Owens

   Article

   Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

   Insert

   His-lacZ-KHHHHHH
   

   Tags

   KHHHHHH and MAHHHHHHSSG

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   May 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET32a-LIC

   Plasmid

   #62310

   Purpose

   E. coli expression vector with N-terminal Trx-tag, 6X His-tag, thrombin site, S-tag, and TEV

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Tags

   6xHis, S-tag, and Trx-Tag

   Expression

   Bacterial

   Promoter

   T7-lacO

   Available Since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAJM.657

    Plasmid

    #108525

    Purpose

    CymRAM + PCymRC-YFP Reporter. Cuma sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    CymRAM + PCymRC-YFP
    

    Expression

    Bacterial

    Mutation

    CymR: S110G A171V

    Available Since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-28a-hPKM2

    Plasmid

    #44242

    Depositor

    Matthew Vander Heiden, Lewis Cantley

    Article

    Christofk et al Nature. 2008 Mar 13;452(7184):181-6. doi

    Insert

    Pyruvate Kinase M2 (PKM Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu

    Plasmid

    #216421

    Purpose

    Empty backbone to express gene specific gRNA and carry the homology repair template for CRISPR editing of Plasmodium berghei using the PbHiT system.

    Depositor

    Ellen Bushell

    Article

    Jonsdottir et al bioRxiv 2024.04.20.590404

    Type

    Empty backbone

    Use

    CRISPR; Expression in plasmodium berghei

    Tags

    3xmyc

    Expression

    Bacterial

    Available Since

    April 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPbU6_hdhfr/yfcu

    Plasmid

    #216422

    Purpose

    Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter for traditional CRISPR editing.

    Depositor

    Ellen Bushell

    Article

    Jonsdottir et al bioRxiv 2024.04.20.590404

    Type

    Empty backbone

    Use

    CRISPR; Expression in plasmodium berghei

    Expression

    Bacterial

    Available Since

    April 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTP1174

    Plasmid

    #104162

    Purpose

    Bacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (1x Cysteine)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-mouse kappa chain specific nanobody TP1170 (1xCysteine)
    

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. MBP-CHC(1-1074)-His6

    Plasmid

    #100748

    Purpose

    Bacterial expression of MBP-tagged clathrin heavy chain large fragment including N-terminal domain, his tagged at C-terminus for purification.

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    CLTC (CLTC Human)
    

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    Residues 1-1074

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. p006-Banana-Late

    Plasmid

    #112251

    Purpose

    ATF1 from Saccharomyces cerevisiae under osmY stationary phase promoter; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    ATF1 under osmY promoter
    

    Expression

    Bacterial

    Promoter

    osmY

    Available Since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNCS mRFP

    Plasmid

    #91765

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Red Fluorescent Protein1 (mRFP1)

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2002 Jun 11;99

    Insert

    monomeric Red Fluorescent Protein1
    

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNCST-AvicFP1

    Plasmid

    #129504

    Purpose

    Expresses AvicFP1 constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AvicFP1
    

    Expression

    Bacterial

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pACM4

    Plasmid

    #49797

    Purpose

    ePathBrick vector for pathway engineering in E. coli.

    Depositor

    Mattheos Koffas

    Article

    Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

    Type

    Empty backbone

    Tags

    S-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDiGi

    Plasmid

    #59323

    Purpose

    Inducible expression of dsRed and GFP in bacteria

    Depositor

    Sophie Helaine, David Holden

    Article

    Helaine et al Proc Natl Acad Sci U S A. 2010 Feb 23;10

    Inserts

    EGFP
    dsRed
    

    Expression

    Bacterial

    Promoter

    Pbad and Plac

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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