33,906 results
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Plasmid#99020PurposeBacterial expression of SF2DepositorAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pCG004
Plasmid#87377PurposeORF part assembly entry vector derived from pHT01.DepositorInsertGFP dropout part
ExpressionBacterialAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19-T7-3WJdB-T
Plasmid#87308PurposeExpresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the T7 promoter.DepositorInsertThree-way junction dimeric Broccoli aptamer
UseSynthetic BiologyExpressionBacterialPromoterT7Available sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBZCas13b-HEPN
Plasmid#89901PurposeBacterial expression for bzCas13b and crRNA with both HEPN domains mutated. New spacers can be cloned by digesting with BsaI.DepositorInsertCas13b
ExpressionBacterialMutationR116A/H121A/R1177A/H1182APromoterLacAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPbcsx28
Plasmid#89909PurposeExpresses csx28 from P. buccae ATCC 33574 in bacteria. Expression driven from the Lac promoterDepositorInsertcsx28
ExpressionBacterialPromoterLacAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTcI38-Wasabi
Plasmid#86117PurposemWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI38DepositorInsertsTcI38
mWasabi
Tags6xHisExpressionBacterialMutationA67T, M1V, L65S, K68R, F115L, D126G, D188GPromoterpR/pL and pR/pL, LacIAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET32bplus_trxAChEwt
Plasmid#83916PurposeEncodes an E. coli codon optimized version of wild type human acetylcholinesteraseDepositorInsertShort form of hAChE, codon optimised for E.coli expression (ACHE Synthetic, Human)
TagsHis, S-tag, and TRXExpressionBacterialMutationcodon optimised for E.coli expressionPromoterT7 promoterAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-ligase-cTF
Plasmid#87743PurposeIPTG-inducible expression of ligase-cTF fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
Tags6xHis and cTF (chimeric transcription factor base…ExpressionBacterialPromoterT5-lacAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET30-2-PHGDH
Plasmid#83907Purposestable overexpressionDepositorInsertPhosphoglycerate dehydrogenase (PHGDH Human)
Tags6x His tagsExpressionBacterialPromoterT7Available sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD/His-miRFP709
Plasmid#79986PurposeMonomeric near-infrared fluorescent protein miRFP709 in bacterial plasmidDepositorInsertmiRFP709
ExpressionBacterialPromoteraraBAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET22b_EcSSB_1
Plasmid#78203PurposeExpression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensorDepositorInsertssb (G26C)
ExpressionBacterialMutationG26C (please see depositor comment below)PromoterT7Available sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3XFLAG-wtGATA6-3XAU1
Plasmid#72612PurposeGateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged human wtGATA6DepositorInsertGATA binding protein 6 (GATA6 Human)
UseGateway CloningTags3XAU1 and 3XFLAGExpressionBacterialPromoterTRE tight promoterAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSPORT1[attB/Ins1/NbT promoter/glGFP/bglobin 3'-UTR/Ins2]
Plasmid#74102Purposeplasmid driven under neuronal beta-tubulin promoter, bearing attB and two insulator sequences (opposite directions pointing inward towards the reporter gene)DepositorInsertsXenopus laevis beta-tubulin gene promoter region and 5'UTR
Green Lantern Green Fluorescent Protein
Rabbit beta 1-globin gene 3'UTR
ExpressionBacterialAvailable sinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
RHOA
Plasmid#73231PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRHOA (RHOA Human)
TagsHis-6-TEVExpressionBacterialMutationpfam00071:Ras 7-180PromoterT7Available sinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Grp1-PH pGEX2TK
Plasmid#71379Purposebacterial expression of PH domain for phosphoinositide binding assayDepositorInsertGrp1-PH (Cyth3 Mouse)
TagsGSTExpressionBacterialMutationPleckstrin homology (PH) domain; aa 264-391PromotertacAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCORE-UH
Plasmid#72239PurposeContains the CORE cassette with KlURA3 and hyg markersDepositorPublicationInsertsKlURA3
hyg
ExpressionBacterialAvailable sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
+36GFP-LPETG
Plasmid#71754Purpose+36GFP-LPETG for expression in E.ColiDepositorInsert(GGS)9_(pos36GFP)_6XHis_LPETG
Tags6XHis and Sortase (LPETG)ExpressionBacterialAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HsSENP2
Plasmid#71466PurposeEncodes human full length SENP2 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 2 (SENP2 Human)
TagsHexahistidineExpressionBacterialMutationGene optimised for E. coli expression (Genscript)PromoterT7Available sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-HsSENP1
Plasmid#71465PurposeEncodes human full length SENP1 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 1 (SENP1 Human)
TagsHexahistidineExpressionBacterialMutationGene optimised for E. coli expression (Genscript)PromoterT7Available sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFGM1
Plasmid#64949Purposesource of GmR FRT cassetteDepositorPublicationInsertFRT-GmR-FRT
ExpressionBacterialAvailable sinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by