29,729 results

1. pEB1029

   Plasmid

   #22066

   Depositor

   Emmanuelle Bouveret

   Article

   Battesti et al Proteomics. 2008 Nov . 8(22):4768-71.

   Type

   Empty backbone

   Use

   Tags

   T25-Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYTK050

   Plasmid

   #65157

   Purpose

   Encodes sgRNA Dropout as a Type 234 part to be used in the Dueber YTK system

   Depositor

   John Dueber

   Article

   Lee et al ACS Synth Biol. 2015 Apr 14.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. plRL58

   Plasmid

   #166886

   Purpose

   Optimized M. tuberculosis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634)

   Depositor

   Jeremy Rock

   Article

   Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tet-On

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-6xHis-(pos15)GFP

   Plasmid

   #89248

   Purpose

   [Edit] Expresses GFP with +15 theoretical charge in E.Coli

   Depositor

   David Liu

   Article

   Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

   Insert

   His6-(pos15)-GFP
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNHEJ-Cas12a

   Plasmid

   #158713

   Purpose

   CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis, expresses FnCpf1, NHEJ machinery of M. marinum

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   the MmNHEJ machinery (MMAR_4573, MMAR_4574, and MMAR_4575)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHH0103_TXK-1/1

   Plasmid

   #91539

   Purpose

   Protein expression and purification of human SH3 domain construct TXK-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   TXK-1/1 (TXK Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHH0103_TEC-1/1

   Plasmid

   #91252

   Purpose

   Protein expression and purification of human SH3 domain construct TEC-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   TEC-1/1 (TEC Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHH0103_ITK-1/1

   Plasmid

   #91253

   Purpose

   Protein expression and purification of human SH3 domain construct ITK-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   ITK-1/1 (ITK Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTmpSm

   Plasmid

   #176228

   Purpose

   A template plasmid for amplification of the cI-hok-strA cassette

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

   Insert

   cI of phage lambda, hok of the R1 plasmid, strA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Drp1-EGFP-StrepII

    Plasmid

    #174429

    Purpose

    Expresses human Drp1 Isoform 3 with EGFP at C-terminus in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1
    

    Use

    Tags

    EGFP-StrepII

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYTK-DN2

    Plasmid

    #180283

    Purpose

    Spacer cassette (TU3)

    Depositor

    Andreas Milias-Argeitis

    Article

    Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

    Insert

    ConL2 - Spacer - ConRE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 6his-WT gusA-pET28a+

    Plasmid

    #61156

    Purpose

    N -terminal His tagged wild-type GUS expression vector

    Depositor

    Ichiro Matsumura, Andrew Ellington

    Article

    Matsumura et al Nat Biotechnol. 1999 Jul;17(7):696-701.

    Insert

    gusA
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET-6xHis-(pos14)GFP

    Plasmid

    #89252

    Purpose

    Expresses GFP with +14 theoretical charge in E.Coli

    Depositor

    David Liu

    Article

    Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

    Insert

    His6-(pos16)-GFP
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMSP3545-dCas9Str

    Plasmid

    #153516

    Purpose

    Expresses dCas9str under nisin-inducible nisA promoter in pMSP3545 backbone

    Depositor

    Kimberly Kline

    Article

    Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

    Insert

    dCas9Str
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    nisA

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pQE32-pr-mEosFP (mEosFP-V69T)

    Plasmid

    #99213

    Purpose

    Bacterial expression of the primed conversion capable protein pr-mEosFP (mEosFP-V69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mEosFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    monomeric variant with mutation V69T for primed c…

    Promoter

    T5

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET41b+_Ufd1-His

    Plasmid

    #117107

    Purpose

    Production of recombinant Ufd1-His in E.coli

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    Ufd1 (UFD1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-21a(+)-AK1-His

    Plasmid

    #118977

    Purpose

    Bacterial expression plasmid for Adenylate kinase isoenzyme 1

    Depositor

    Sebastian Maerkl

    Article

    Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

    Insert

    adenylate kinase isoenzyme 1 AK1 (AK1 Chicken)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. KillerOrange /pQE-30

    Plasmid

    #74748

    Purpose

    KillerOrange gene for bacterial expression

    Depositor

    Konstantin Lukyanov

    Article

    Sarkisyan et al PLoS One. 2015 Dec 17;10(12):e0145287. d

    Insert

    KillerOrange
    

    Use

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    KillerRed with substitutions G3C Y66W D113S N145S…

    Promoter

    T5

    Available since

    Aug. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLG012 (DRT type 4)

    Plasmid

    #157890

    Purpose

    Expresses the DRT type 4 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    DRT type 4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLG013 (DRT type 5)

    Plasmid

    #157891

    Purpose

    Expresses the DRT type 5 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    DRT type 5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge