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Bacterial Expression

29,729 results

Showing: 3381 - 3400 of 29729 results

  1. Biomolecular FeedBack (FB)

    Plasmid
    #109391
    Purpose
    This construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.
    Depositor
    Tom Ellis
    Article
    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463
    Insert
    pJ23100mut-dCas9-T- phtpG1-sgRNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    109391-plasmid-map-sequence-id-210784

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  6. pUCmEos3.2:cat

    Plasmid
    #78100
    Purpose
    Plasmid template that encodes the photoconvertible fluorescent protein mEos3.2 next to chloramphenicol resistance gene (cat). This template was used to copy out mEos3.2-cat for genetic tagging.
    Depositor
    Peng Chen
    Article
    Chen et al Nat Commun. 2015 Jul 6;6:7445. doi: 10.1
    Insert
    mEos3.2-cat
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 7, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. pUS250

    Plasmid
    #198322
    Purpose
    Cumate-inducible gene expression in diverse gram negative bacteria. Features also include mobilisation via oriT, a gigantic multiple cloning site, and blue/white screening via amilCP chromoprotein
    Depositor
    Nicholas Coleman
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    cumate-inducible
    Available since
    April 12, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    198322-plasmid-map-sequence-id-390064

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  13. pET23a_mod_HuD_FL

    Plasmid
    #65751
    Purpose
    plasmid for bacterial overexpression of HuD
    Depositor
    Thomas Tuschl
    Insert
    ELAVL4 (ELAVL4 Human)
    Use
    Tags
    6X His and FLAG HA
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65751-plasmid-map-sequence-id-114929

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  14. pUB-MSP1D1

    Plasmid
    #161609
    Purpose
    Bacterial expression of mutated membrane scaffold protein ("ultra-bright" MSP1D1) for Nanodisc formation
    Depositor
    Stephen Sligar
    Article
    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do
    Insert
    UD-MSP1D1
    Use
    Tags
    7-His tag
    Expression
    Bacterial
    Mutation
    mutant of MSP1D1 ( Q138W, L181W, Y236W)
    Promoter
    T7
    Available since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    161609-plasmid-map-sequence-id-317666

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  15. D. vulgaris sp2 CRISPR/pACYCDuet-1

    Plasmid
    #81186
    Purpose
    Expresses CRISPR array containing 3 copies of Desulfovibrio vulgaris spacer 2 and flanking repeats in E. coli
    Depositor
    Jennifer Doudna
    Article
    Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:
    Insert
    Synthetic CRISPR array (3 copies of D. vulgaris spacer #2 flanked by repeats)
    Use
    CRISPR
    Tags
    None
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    81186-plasmid-map-sequence-id-162820

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  16. pET23b-2E2-HA-mEGFP-6xHis

    Plasmid
    #129594
    Purpose
    The encoded protein is the anti-HA frankenbody variant fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
    Use
    Tags
    6xHis-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    129594-plasmid-map-sequence-id-261157

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  17. pOPARA1

    Plasmid
    #200416
    Purpose
    Golden Gate assembly of expression constructs containing pBAD promoter (carbenicillin resistance)
    Depositor
    Kamil Witek
    Article
    Lesley et al bioRxiv
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200416-plasmid-map-sequence-id-394143

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  19. A1-LCD+7R+12D

    Plasmid
    #178678
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added, twelve Glu residues added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD+7R+12D (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    seven Arg residues added, twelve Glu residues add…
    Promoter
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    178678-plasmid-map-sequence-id-357023

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Showing: 3381 - 3400 of 29729 results