30,744 results

1. pCMV-Spike

   Plasmid

   #173921

   Purpose

   Expresses the original SARS-Cov-2 Spike Protein under a CMV Promoter

   Depositor

   Andreas Stuermer

   Insert

   SARS-2 Spike Protein (S Human, Synthetic, SARS-COV-2)
   

   Tags

   no tags, native protein

   Expression

   Bacterial and Mammalian

   Promoter

   standard CMV

   Available Since

   April 11, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pET28_6xHis-PARP1-CAT

   Plasmid

   #173943

   Purpose

   Bacterial expression of isolated PARP1 CAT domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-CAT (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBTK1009 (pSL9)

   Plasmid

   #191004

   Purpose

   Expresses E2 Crimson on a broad host range pBBR1 origin plasmid

   Depositor

   Jeffrey Barrick

   Article

   Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

   Insert

   E2-Crimson
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   CP25

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET-45b-mEGFP-HMGB1-WT

   Plasmid

   #194543

   Purpose

   Bacterial Expression of 6xHis mEGFP-HMGB1 WT Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

   Insert

   HMGB1 (HMGB1 Human)
   

   Tags

   mEGFP

   Expression

   Bacterial

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HHM-iATPSnFR1.0

   Plasmid

   #102546

   Purpose

   expresses ATP sensor in bacteria

   Depositor

   Baljit Khakh

   Article

   Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

   Insert

   iATPSnFR1.0
   

   Expression

   Bacterial

   Available Since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PET-B-Sniper SpCas9-NLS-6xHis

   Plasmid

   #207377

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-Sniper SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F539S, M763I, K890N, amino acids 1005-1013 replac…

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. His-PIMT

   Plasmid

   #34852

   Depositor

   Bruce Downie

   Article

   Dinkins et al Plant J. 2008 Jul;55(1):1-13.

   Insert

   PIMT (PCMT1 Human)
   

   Tags

   6XHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

8. IBMc396

   Plasmid

   #161954

   Purpose

   Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHH

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T
   

   Expression

   Bacterial

   Promoter

   PBAD

   Available Since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX6-Smad3 WT

   Plasmid

   #27010

   Depositor

   Joan Massague

   Article

   Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

   Insert

   Smad3 (SMAD3 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

10. PUC57_BAG3wt-eGFP

    Plasmid

    #98182

    Purpose

    Codes for full length, GFP tagged, human wildtype Bag3

    Depositor

    Robert Bryson-Richardson

    Article

    Ruparelia et al Acta Neuropathol. 2014 Dec;128(6):821-33

    Insert

    BAG3wt-eGFP (BAG3 Human)
    

    Tags

    GFP

    Expression

    Bacterial

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pZ8-Ptac

    Plasmid

    #74064

    Purpose

    IPTG inducible version of pZ8-1 plasmid, to which lacIq was added, KanR

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    ptac

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pATetO 6XHis-MMLV RT

    Plasmid

    #178797

    Purpose

    Inducible expression of MMLV RT in E. coli

    Depositor

    Andrew Ellington

    Article

    Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

    Insert

    MMLV RT
    

    Expression

    Bacterial

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJBL7010

    Plasmid

    #136942

    Purpose

    Constitutive sfGFP expression (high), bacterial promoter

    Depositor

    Julius Lucks

    Article

    Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GB1-A11(2-196, D40G)-Strep

    Plasmid

    #196210

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, D40G)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Mutation

    D40G

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET mOrange LIC cloning vector (u-mOrange)

    Plasmid

    #29770

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    mOrange

    Expression

    Bacterial

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pK27Sumo_His-SUMO-nsp7 (SARS-CoV-2)

    Plasmid

    #169186

    Purpose

    To express His-SUMO-nsp7 (SARS-CoV-2) in E. coli

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    14His-SUMO-nsp7 (ORF1ab SARS-CoV-2)
    

    Tags

    14His-SUMO (Ulp1-cleavable)

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SIRT1.1

    Plasmid

    #13735

    Depositor

    John Denu

    Article

    Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

    Insert

    SIRT1 (SIRT1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pProEx RalA(G23C) CL

    Plasmid

    #224263

    Purpose

    Protein purification of RalA (G23C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    RalA (RALA Human)
    

    Expression

    Bacterial

    Mutation

    G23C

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTriEx-mCherry-PA-Rac1-C450A

    Plasmid

    #22028

    Purpose

    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
    

    Tags

    6xHis and mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV(Leu404-Leu546, C450A). Rac1 starts at Ile4 an…

    Promoter

    CMV, p10

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMGE-T7

    Plasmid

    #153021

    Purpose

    Single-plasmid multi-gene expression system. Each genes under the control of a T7 and the lacO promoter sequences.

    Depositor

    Vito Valiante

    Article

    Kufs et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge