31,624 results
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Plasmid#135188PurposeIntegration and excision of chloromaphenicol cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection.DepositorInsertCmR
UseSynthetic BiologyExpressionBacterialMutationNonePromotercatAvailable SinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEK39
Plasmid#210599PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Bpro-Hshph-G4S-HsmRuby3-ScADH1ter
TagsmRuby3ExpressionBacterial and YeastPromoterSpH2BproAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
dCas9-CRY2Clust-BRD4ΔN
Plasmid#200968Purposeinduction of phase-separated condensate mediated by BRD4dN-IDRDepositorInsertBRD4dN
ExpressionBacterial and MammalianAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-32a(+)-TGFB1m
Plasmid#120286PurposeProduce human recombinant mTGFB1 in DE3 cellsDepositorInsertmonomeric Transforming Growth Factor-β1
Tags6x His and S-TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 PromotorAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCW-LIC-N8C_Fab3a
Plasmid#203689PurposeExpresses N8C_Fab3aDepositorInsertN8C_Fab3a
ExpressionBacterialAvailable SinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET30a-YedK
Plasmid#86884PurposeBacterial expression of E. coli SRAP ortholog YedKDepositorAvailable SinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK792
Plasmid#8830DepositorInsertTEV protease, S219P mutant
TagsHis, MBP (cleaved), and polyarginineExpressionBacterialMutationS219P mutationAvailable SinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET-EIN-FTO(32-505)
Plasmid#186466PurposeExpresses human RNA demythelase protein FTO 32-505 amino acid sequence with N-terminal fusion with the N-domain of EI ( e.coli protein)DepositorInsertFat mass obesity associated protein (FTO Human)
Tags6 Histidine-EIN (N-terminal domain of E.coli Enzy…ExpressionBacterialMutationamino acid 32-505PromoterT7Available SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHS71_10xHis_MBP_TEV_WT-Cas9_2xNLS
Plasmid#244833PurposeBacterial expression of SpyCas9 (WT) with 2xSV40 nuclear localization sequence for protein purificationDepositorInsertSpyCas9 (WT)
UseCRISPRTags10xHis-MBP and 2xSV40 NLSExpressionBacterialPromoterT7Available SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHS1_10xHis_MBP_TEV_WT-Cas9
Plasmid#244825PurposeBacterial expression of SpyCas9 (WT) for protein purificationDepositorInsertSpyCas9 (WT)
UseCRISPRTags10xHis-MBPExpressionBacterialPromoterT7Available SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
NPY-GFP
Plasmid#74629Purposehuman neuropeptide Y fused to GFPDepositorAvailable SinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK172-6xHis-0N4R-P301S-tau-HiBiT
Plasmid#187835PurposeExpression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.DepositorInsert6XHis-0N4R-P301S-tau-HiBiT
Tags6xHistidine and HiBiTExpressionBacterialPromoterT7Available SinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET Flag TEV LIC cloning vector (1L)
Plasmid#29662DepositorTypeEmpty backboneTagsFlag and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCCM’
Plasmid#162709PurposepCCM reconstructed after selection for CCMB1 growth on glycerol under ambient airDepositorInsertSecond CCM operon cloned from H. neapolitanus
ExpressionBacterialMutationp15A origin replaced with high-copy colE1 originPromoterPLtet0-1 promoterAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-cMSP1E3D1
Plasmid#199772PurposeExpresses split-intein fused MSP1E3D1 for circularization during expression (external His-Tag)DepositorInsertcMSP1E3D1
TagsHis6-TagExpressionBacterialMutationExtended cMSP1D1; external His-Tags flanking the …Available SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisD-moxMaple3
Plasmid#120871PurposeBacterial expression of moxMaple3DepositorInsertmoxMaple3
TagsHisExpressionBacterialMutationfrom mMaple3, moxMaple3 was engineered by replaci…Available SinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP3
Plasmid#79875PurposeBacillus subtilis sgRNA expression vector; integrates into thrCDepositorInsertsgRNA RR1
UseCRISPRExpressionBacterialPromotervegAvailable SinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
HMGCS2
Plasmid#38984PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSB219
Plasmid#164125Purposep15A backbone with PhlF binding sitesDepositorInsertsfGFP
ExpressionBacterialPromoterJ23105Available SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1-Optimized UBE1L
Plasmid#165099Purposeecoli optimized expression of GST-fusion of human Ube1LDepositorInsertUbeE1L (UBA7 Human)
TagsGST from plasmid.ExpressionBacterialMutationcodon optimized for E. coli expressionAvailable SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only