30,261 results

1. pQE30-mBFP

   Plasmid

   #112682

   Purpose

   Expression of NADPH-dependent blue fluorescent protein

   Depositor

   Geun-Joong Kim

   Article

   Hwang et al Biochem Biophys Res Commun. 2012 Mar 23;

   Insert

   His-mBFP
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-21(+)Tm9D8* TrpB

   Plasmid

   #207517

   Purpose

   Expression of Tm9D8* TrpB for the synthesis of 7-Aza-L-Tryptophan (7AW) from 7-Azaindole (7AI) and serine

   Depositor

   Thomas Huber

   Article

   Abdelkader et al ACS Sens. 2023 Oct 27. doi: 10.1021/acss

   Insert

   Tryptophan synthase β-subunit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCDM4

   Plasmid

   #49796

   Purpose

   ePathBrick vector for pathway engineering in E. coli.

   Depositor

   Mattheos Koffas

   Article

   Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

   Type

   Empty backbone

   Use

   Tags

   S-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNR276

   Plasmid

   #79577

   Purpose

   TP901 attB-attP. GFP will express if sites recombine.

   Depositor

   Timothy Lu

   Article

   Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

   Insert

   GFPmut3b
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNR239

   Plasmid

   #79576

   Purpose

   A118 attB-attP. GFP will express if sites recombine.

   Depositor

   Timothy Lu

   Article

   Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

   Insert

   GFPmut3b
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET50_WT MDA5

   Plasmid

   #216802

   Purpose

   Expresses wild type (wt) melanoma differentiation-associated protein 5

   Depositor

   Sun Hur

   Insert

   melanoma differentiation-associated protein 5 (IFIH1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET151-GyrB

   Plasmid

   #216233

   Purpose

   S. aureus USA300 GyrB Codon-optimized for E. coli

   Depositor

   Eric Brown

   Article

   French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

   Insert

   DNA gyrase
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJSC114 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant

   Plasmid

   #101215

   Purpose

   Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant K848A/K1003A/R1060A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   K848A, K1003A and R1060A

   Promoter

   T7

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSG66-KcsA-Kv1.3-sfGFP

   Plasmid

   #102457

   Purpose

   Expresses a fusion protein of KcsA-Kv1.3 and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.

   Depositor

   Richard Murray

   Article

   Guo et al bioRxiv 104455

   Insert

   KcsA-Kv1.3
   

   Use

   Tags

   His tag and sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   OR2-OR1-Pr

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHLUM (version 2)

    Plasmid

    #64166

    Purpose

    harbours marker genes HIS3, LEU2, URA3 and MET17

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHH0103_TP53BP2-1/1

    Plasmid

    #91503

    Purpose

    Protein expression and purification of human SH3 domain construct TP53BP2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    TP53BP2-1/1 (TP53BP2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDuet TDP-43 M337V

    Plasmid

    #27464

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Coexpression

    Tags

    Expression

    Bacterial

    Mutation

    Methionine 337 mutated to Valine (M337V)

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

13. Keasling-2337

    Plasmid

    #100168

    Purpose

    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

    Depositor

    Jay Keasling

    Article

    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

    Insert

    lacIq-Ptrc-ADS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST-hisMBP-AsCpf1-EC

    Plasmid

    #79007

    Purpose

    Expressing his-MBP tagged AsCpf1 (E.coli codon optimized)

    Depositor

    Jin-Soo Kim

    Article

    Hur et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Insert

    AsCpf1
    

    Use

    Tags

    6xHis-MBP-NLS and NLS-HA

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLG007 (Ec86 retron)

    Plasmid

    #157885

    Purpose

    Expresses the Ec86 retron defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    Ec86 retron
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNMT

    Plasmid

    #42578

    Depositor

    James Hurley

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

    Insert

    N-myristoyl tranferase (NMT1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    two silent mutations T690C and T942C

    Promoter

    T7

    Available since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pET24a-LaM4-EGFP

    Plasmid

    #182641

    Purpose

    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to EGFP. LaM4-EGFP also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

    Insert

    anti-mCherry nanobody fused to a T7, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, EGFP, HA, His6, and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-SET

    Plasmid

    #234708

    Purpose

    expresses His-tagged human SET in E coli

    Depositor

    Lienhard Schmitz

    Article

    Seibert et al J Cell Biol. 2019 Apr 1;218(4):1164-1181

    Insert

    SET (SET Human)
    

    Use

    Tags

    (His)6

    Expression

    Bacterial

    Mutation

    Promoter

    lacI

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. Venus-pBAD

    Plasmid

    #54859

    Purpose

    Localization: Protein Expression Vector , Excitation: 515, Emission: 528

    Depositor

    Michael Davidson, Atsushi Miyawaki

    Article

    Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

    Type

    Empty backbone

    Use

    Tags

    6xHis and Venus

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pQE-80L MBP-SspB Nano

    Plasmid

    #60409

    Purpose

    E. coli expression of SspB Nano

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    SspB nano
    

    Use

    Tags

    His-MBP-TEV

    Expression

    Bacterial

    Mutation

    Y11K and A15E

    Promoter

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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