29,740 results

1. pAJM.336

   Plasmid

   #108528

   Purpose

   LacIAM + PTac-YFP Reporter. IPTG sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   LacIAM + PTac-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LacI Q18M A47V F161Y

   Promoter

   Available since

   July 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTD103-ARG1

   Plasmid

   #106475

   Purpose

   Expresses acoustic reporter genes or gas vesicles (ARG1) in Salmonella typhimurium

   Depositor

   Mikhail Shapiro

   Article

   Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

   Insert

   ARG1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLux

   Available since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPROEX-HTc-Flag3-Nrf2

   Plasmid

   #21553

   Depositor

   Yue Xiong

   Article

   Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

   Insert

   nuclear factor (erythroid-derived 2)-like 2 (NFE2L2 Human)
   

   Use

   Tags

   6xHis and Flag3

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 1, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pET28a-His-hAos1

   Plasmid

   #53135

   Purpose

   E.coli expression of full length human Aos1 (SAE1) with Nterminal His-tag

   Depositor

   Frauke Melchior

   Article

   Pichler et al Cell. 2002 Jan 11;108(1):109-20.

   Insert

   Aos1 (SAE1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRE-Tn7-142

   Plasmid

   #118558

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   sGFP2
   

   Use

   Minitn7 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLG032 (ppl)

   Plasmid

   #157910

   Purpose

   Expresses the ppl defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   ppl (PHP-ATPase)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS hShh (CT#401)

   Plasmid

   #13996

   Depositor

   Cliff Tabin

   Article

   Marigo et al Genomics. 1995 Jul 1. 28(1):44-51.

   Insert

   Shh (SHH Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPSV39-dddAI

   Plasmid

   #186557

   Purpose

   Broad host range regulated expression of the DddA immunity determinant (DddAI)

   Depositor

   Joseph Mougous

   Article

   Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

   Insert

   dddAI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. PET-WT SpCas9-NLS-6xHis

   Plasmid

   #207373

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   WT SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBJ4

    Plasmid

    #167133

    Purpose

    A kanamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

    Depositor

    Akira Mine

    Article

    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

    Insert

    luxCDABE
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Kanamycin promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKERep

    Plasmid

    #211826

    Purpose

    Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKESa

    Plasmid

    #211828

    Purpose

    Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBbA6c_PsulA_sfGFP

    Plasmid

    #224587

    Purpose

    SOS response reporter

    Depositor

    Ophelia Venturelli

    Article

    Cheng et al Mol Syst Biol. 2023 Mar 9;19(3):e11406.

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PsulA

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-2T_EV-D68_VP1

    Plasmid

    #202072

    Purpose

    Bacterial expression of VP1 protein from enterovirus D68 (EV-D68). Contains N-terminal GST-tag and C-terminal His6-tag.

    Depositor

    Vesa Hytönen

    Article

    Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

    Insert

    EV-D68 VP1 protein with Histag
    

    Use

    Tags

    6xHis-tag and GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. 6920 pPROEXHsp104b

    Plasmid

    #1230

    Depositor

    Susan Lindquist

    Article

    Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

    Insert

    Hsp104 (HSP104 Budding Yeast)
    

    Use

    Tags

    his tag (tev cleavable)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 15, 2005

    Availability

    Academic Institutions and Nonprofits only

16. pSCW02

    Plasmid

    #72301

    Purpose

    Starting substrate for in vitro DNA mismatch repair substrate monitored by FauI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

    Depositor

    Peggy Hsieh

    Article

    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

    Type

    Empty backbone

    Use

    Derivative of puc19

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. M57 pPET28a-TEV-full-length human beta catenin

    Plasmid

    #17198

    Depositor

    Randall Moon

    Insert

    beta catenin (CTNNB1 Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pWSK129-stm2585

    Plasmid

    #174401

    Purpose

    S. Typhimurium stm2585 (sarA/steE) flanked with endogenous regulatory sequences (786bp on 5' and 28bp on 3' end) for bacterial expression.

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    sarA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. KLHL2

    Plasmid

    #39060

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    KLHL2 (KLHL2 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pTHSSe_46

    Plasmid

    #109244

    Purpose

    pSC101 var4 BbsI cloning vector

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23105

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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