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pET3-ARF6-His6(CO)

Plasmid

#201887

Purpose

Used for bacterial expression of human protein ARF6

Depositor

Richard Kahn

Insert

ARF6 (ARF6 Human)

Tags

6xHis (GSGSGSHHHHHH)

Expression

Bacterial

Mutation

Codon optimized for bacterial expression

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_GNAI2_G-alpha

Plasmid

#109791

Purpose

Protein expression and purification of GNAI2_G-alpha

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNAI2_G-alpha (GNAI2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pQLinkH

Plasmid

#13667

Depositor

Konrad Buessow

Article

Scheich et al Nucleic Acids Res. 2007;35(6):e43. doi:

Insert

multi cloning site

Use

Co-expression

Tags

His, TEV, and attB1

Expression

Bacterial

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

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pLQ-Pxyl/tet-cas9-Pj23119-sgRNA

Plasmid

#92121

Purpose

CRISPR-Cas9 based efficient genome editing in S. aureus

Depositor

Sheng Yang

Article

Liu et al Acta Biochim Biophys Sin (Shanghai). 201

Insert

Cas9-Pxyltet-sgRNA-pj23119

Use

CRISPR

Expression

Bacterial

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pN565

Plasmid

#49990

Purpose

expresses an attenuated T7 RNAP under an isopropyl β-d-1-thiogalactopyranoside (IPTG) inducible control

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

T7 RNAP*

Use

Synthetic Biology

Tags

umuD degradation tag

Expression

Bacterial

Mutation

R632S

Promoter

pTac-symmetric

Available Since

Jan. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-161

Plasmid

#118541

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSB233

Plasmid

#164128

Purpose

PP7-CFP and PhlF-RFP expression plasmid

Depositor

Christopher Voigt

Article

Shao et al Nat Commun. 2021 Mar 5;12(1):1475. doi:

Insert

PP7-CFP

Expression

Bacterial

Promoter

Ptet

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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PUC57_BAG3wt-eGFP

Plasmid

#98182

Purpose

Codes for full length, GFP tagged, human wildtype Bag3

Depositor

Robert Bryson-Richardson

Article

Ruparelia et al Acta Neuropathol. 2014 Dec;128(6):821-33

Insert

BAG3wt-eGFP (BAG3 Human)

Tags

GFP

Expression

Bacterial

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pET mOrange LIC cloning vector (u-mOrange)

Plasmid

#29770

Depositor

Scott Gradia

Type

Empty backbone

Tags

mOrange

Expression

Bacterial

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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hsDCX-EGFP-pHAT

Plasmid

#83918

Purpose

human Doublecortin, N-terminal 6-His tag, C-terminal EGFP-Strep-tag II

Depositor

Gary Brouhard

Article

Bechstedt et al Dev Cell. 2012 Jul 17;23(1):181-92. doi:

Insert

Doublecortin isoform b (DCX NM_178152.2, Human)

Tags

6-His and EGFP+Strep-tag II

Expression

Bacterial

Available Since

Dec. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pH6HTC_HSPA9-Halo

Plasmid

#175332

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HSPA9 (HSPA9 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pSLIK CA RhoA

Plasmid

#112894

Purpose

promoter-inducible expression of constitutively-active RhoA

Depositor

Sanjay Kumar

Article

MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

Insert

CA RhoA (RHOA Human)

Use

Lentiviral

Expression

Bacterial

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pMM656

Plasmid

#68887

Purpose

Rhamnose-inducible NanoLuc expression, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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His-TEV-PRC1

Plasmid

#167539

Purpose

For expression of His6-TEV-PRC1 in bacteria.

Depositor

Tarun Kapoor

Article

Subramanian et al Cell. 2013 Jul 18;154(2):377-90. doi: 10

Insert

PRC1 (PRC1 Human)

Tags

His6 tag

Expression

Bacterial

Promoter

T7

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pQTEV-PSMD11

Plasmid

#31333

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

proteasome (prosome, macropain) 26S subunit, non-ATPase, 11 (PSMD11 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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SAP145

Plasmid

#11239

Depositor

Robin Reed

Article

Gozani et al Genes Dev. 1996 Jan 15. 10(2):233-43.

Insert

SAP145 (SF3B2 Human)

Expression

Bacterial

Mutation

First 7 amino acids are missing compared to NP_00…

Available Since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only

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SPLICSFAST-NU-MT-P2A

Plasmid

#214898

Purpose

Expresses equimolar Nucleus- and Mitochondria- targeted split-FAST fragments for Short-range NU-MT Contact sites

Depositor

Tito Calì

Insert

SPLICSFAST-NU-MT-P2A

Tags

none

Expression

Bacterial and Mammalian

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor14-AcHELIX-KanR (CJT96)

Plasmid

#181794

Purpose

pDonor for AcHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

KanR, R6K origin of replication

Expression

Bacterial

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pMC_MS2_DNA_VLP

Plasmid

#179156

Purpose

Expression of maturation protein and his-tagged coat protein dimer from MS2 phage

Depositor

Paul Freemont

Article

Crone et al GEN Biotechnol. 2022 Dec 1;1(6):496-503.

Inserts

Maturation Protein
Coat Protein Dimer

Expression

Bacterial

Promoter

T7

Available Since

Sept. 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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phage UbiC G3BP1-SNAP

Plasmid

#119949

Purpose

Lentiviral vector expressing G3BP1-SNAP fusion protein

Depositor

Jeffrey Chao

Article

Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

G3BP1 (G3BP1 Human)

Use

Lentiviral

Tags

SNAP

Expression

Bacterial and Mammalian

Promoter

UbiC

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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