30,753 results
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Plasmid#190610PurposeExpresses the RamR transcription factor in E. coliDepositorInsertRamR
ExpressionBacterialAvailable SinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
GST-thr-RAF1-RBD
Plasmid#86033PurposeExpresses the RAS-binding domain of RAF1 for in vitro analysis of RAS activationDepositorInsertRAF1 RBD (Ras-binding domain) (RAF1 Human)
TagsGSTExpressionBacterialMutationContains residues 1-149PromoterT7Available SinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
BK Dunlop
Plasmid#25466DepositorInserthuman papovavirus BK Dunlop
ExpressionBacterialAvailable SinceSept. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-HA
Plasmid#119756PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal HA tag.DepositorTypeEmpty backboneTagsGST and HAExpressionBacterialPromotertac promoterAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSWAP_Entry_T2
Plasmid#184864PurposepSwap plasmid part containing the T2 sgRNA module for the Swap and Drop recombination system.DepositorInsertlacZalpha,ccdB
ExpressionBacterialAvailable SinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSWAP_Entry_T1
Plasmid#184863PurposepSwap plasmid part containing the T1 sgRNA module for the Swap and Drop recombination system.DepositorInsertlacZalpha,ccdB
ExpressionBacterialAvailable SinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET11a-SUMO3
Plasmid#53143PurposeE.coli expression of untagged mature human SUMO3 (amino acids 1-93)DepositorAvailable SinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOPINS-USP21 (USP, aa 196-565)
Plasmid#61585PurposeExpresses human USP21 (USP domain) in E. coli.DepositorInsertUSP21 (Ubiquitin carboxyl-terminal hydrolase 21) (USP21 Human)
TagsHis6-SUMOExpressionBacterialMutationDeleted aa 1-195.PromoterT7Available SinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSG15-pBAD-tetO-deGFP
Plasmid#102450PurposeExpresses deGFP fluorescent protein in E. coli. The expression is controlled by a pBAD-tetO combinatorial promoter.DepositorInsertdeGFP
ExpressionBacterialPromoterpBAD-tetOAvailable SinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMJ841
Plasmid#39318DepositorInsertCas9
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationAspartate 10 to Alanine (D10A) and Histidine 840 …PromoterT7Available SinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDF-Mm2
Plasmid#197100PurposeTo express the variant of Methanosarcina mazei PylRS specific for pyrrolysine derivatives (Boc-Lys, Az-ZLys, AzAmZLys) and its cognate Pyl tRNA and allow UAG codon to be translated into these derivatiDepositorInsertPylRS variant, Pyl tRNA, kanamycin resistance gene
ExpressionBacterialMutationLys61, Glu131, Ala306, Phe384, Glu444 (PylRS)Available SinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDSG290
Plasmid#115609Purposesurface-displayed Nb3, used in composability experiments; low copyDepositorInsertregulated adhesin construct
ExpressionBacterialAvailable SinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
human p53-(94-312)
Plasmid#24866DepositorAvailable SinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pECGMC3
Plasmid#75441PurposePlasmid for mf-lon mediated mCherry degradation in Lactococcus lactis. Contains mcherry-pdt3DepositorInsertmf-lon
ExpressionBacterialPromoternisA promoterAvailable SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHN1002
Plasmid#178831PurposeBacterial expression for human Saposin ADepositorInsertartificial gene encoding human Saposin A (AR Human)
TagsHis8, 3CExpressionBacterialMutationCodon-usage was optimized for bacterial expressio…PromoterT7Available SinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMS73
Plasmid#110629PurposeCRISPR/Cas9 vector for mutliplexed genome editing in Ustilago maydis. Expresses U. maydis codon-optimized Cas9 under the hsp70 promoter, contains a short version of U6 promoter, is self-replicatingDepositorInsertsshort U6 promoter
cas9
tnos terminator
UseCRISPR; Self-replicating in ustilago maydis, conf…TagsN-terminal NLS, C-terminal HA-tag +NLSExpressionBacterialMutationStreptococcus pyogenes gene codon-optimized for U…PromoterU. maydis hsp70 promoter (Kronstad and Leong, 198…Available SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAS1yl
Plasmid#73226PurposeConstitutive expression of Cas9 and sgRNA in Yarrowia lipolytica cellsDepositorInsertsCas9
sgRNA
UseCRISPR; In y. lipolyticaExpressionBacterialPromoterunknownAvailable SinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1 Hepatitis C Virus NS3/NS4A
Plasmid#61696Purposeinducible dual expression of NS3 protease along with its cofactor NS4ADepositorInsertsNS4A
NS3
TagshisExpressionBacterialPromoterT7Available SinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-RspCas13d
Plasmid#108305PurposeExpresses E. coli codon optimized RspCas13d in the pET28a backbone.DepositorInsertRspCas13d
UseCRISPRTagsmH6 tagExpressionBacterialPromoterlacAvailable SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen-Etanercept
Plasmid#182421PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen-Etanercept fusionDepositorInsertVNp-mNeongreen-Etanercept (TNFRSF1B Human)
TagsVesicle Nucleating peptide (VNp) & mNeongreenExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only