32,671 results
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Plasmid#224927PurposeTniQ expression plasmid for the co-purification of TnsC-TniQ complexDepositorInsertTniQ
UseCRISPRExpressionBacterialAvailable SinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80RFP-pXD68Kan2-RFP
Plasmid#225329PurposeRSF1010-based broad host-range plasmid engineered from pXD68Kan2 encoding RFP, kanamycin resistanceDepositorInsertRFP
ExpressionBacterialAvailable SinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
QUAS-5-T7-TetO-GFP_ColE1
Plasmid#171660PurposeQUAS five base pairs upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli according to the Q and Tet systems. Contains the ColE1 origin of replication.DepositorInsertQUAS-5-T7-TetO-GFP ssra-T7 Stop
TagsssrA degradation tag (DAS+4)ExpressionBacterialPromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-QF-T7-TetO-TetR_p15A
Plasmid#171661PurposeExpresses QF and/or TetR in BL21(DE3) E. coli according to the presence of aTc. There is no T7 terminator sequence between the QF- and TetR-containing circuits. Contains a p15A origin of replication.DepositorInsertQF-T7-TetO-TetR
ExpressionBacterialPromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTL2-IncB-GFP11x7
Plasmid#113031PurposeExpresses IncB tagged with 7 copies of GFP11 under a tetracycline inducible promoterDepositorInsertIncB (incB Chlamydia trachomatis)
TagsGFP11x7 and flagExpressionBacterialPromotertet inducibleAvailable SinceOct. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
ASC-CASP1 Octamer
Plasmid#164032PurposeBacterial expression vector encoding a 5GSS-linked ASC(CARD)-CASP1(CARD) construct to express an octamerDepositorTagsHis6-MBP-TEVExpressionBacterialMutationW169G (ASC), G20K (CASP1)Available SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
RNF168
Plasmid#25298PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 16, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXDC61
Plasmid#21841DepositorInsertblaM
ExpressionBacterialAvailable SinceSept. 2, 2009AvailabilityAcademic Institutions and Nonprofits only -
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human p53-(1-393)-L344P
Plasmid#24862DepositorAvailable SinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
H11-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato
Plasmid#180156PurposetdTomato cre reporter with Hipp11 recombineering homology armsDepositorInserttdTomato
UseCre/Lox and Mouse TargetingExpressionBacterial and MammalianAvailable SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
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AfNitB_pET29b
Plasmid#215401PurposeExpression construct for AfNitB gene, codon optimized for expression in E. coliDepositorInsertAfNitB
ExpressionBacterialAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPPC005
Plasmid#171140PurposeFor integration of XylS-Pm-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T methodDepositorInsertXylS-Pm
UseCRISPRExpressionBacterialPromoterPmAvailable SinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN51 (R40A)
Plasmid#80109PurposeLate1-O3-33-myc-PH(R40A)DepositorInsertEPN-51 (R40A)
ExpressionBacterial and MammalianAvailable SinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-YWHAE
Plasmid#31562DepositorInserttyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon (YWHAE Human)
TagsHis and TEVExpressionBacterialAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-51b-HaloTag10
Plasmid#175520PurposeExpression of HaloTag10 in bacterial cellsDepositorInsertHaloTag10
TagsHisExpressionBacterialMutationHaloTag10 = HaloTag7-Q165WAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pET30-T7-His-sfGFP
Plasmid#180755PurposepET30 vector missing lacO with sfGFP-S-tag inserted between the Bgl II and T7 terminatorDepositorInsertsuperfolder green fluorescent protein
Tagsstrep tagExpressionBacterialAvailable SinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEB1-Sapphire
Plasmid#103976PurposePlasmid encoding SapphireDepositorInsertSapphire
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (S72A, Y145F,…PromoterproCAvailable SinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJF3H
Plasmid#63897PurposeFor phage display of cDNA librariesDepositorInsertcDNA cloning region
UsePhage displayTagsFosExpressionBacterialPromoterlacZAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
fSAP24-GST
Plasmid#11202DepositorAvailable SinceJan. 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMVA2
Plasmid#119951PurposeExpresses recombinant MVA pathway in Escherichia coliDepositorInsertsmvaE
mvaS
mvaK1
mvaK2
mvaD
idi
ExpressionBacterialMutationA110GAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIYN3
Plasmid#197099PurposeTo express the variant of M. jannaschii TyrRS specific for halogenated tyrosines and Nap3 amber suppressor tRNA and allow UAG codon to be translated into these derivatives.DepositorInsertTyrRS variant, Nap3 tRNA
ExpressionBacterialMutationAla70, Thr158, Ser159, Arg286 (TyrRS)Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKSS
Plasmid#39272DepositorTypeEmpty backboneUsePositive-selection cloning vectorExpressionBacterialAvailable SinceSept. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PAK2 wt
Plasmid#31671DepositorAvailable SinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pED14xS-4
Plasmid#134802PurposeIPTG inducible expression of S-TAGA-Evo3 qtRNADepositorInsertS-TAGA-Evo3 qtRNA
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher002 EQ
Plasmid#102830PurposeBacterial expression of unreactive version of SpyCatcher002DepositorInsertSpyCatcher002 EQ
TagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationE77Q mutation prevents isopeptide bond formationPromoterT7Available SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet1_6xHis-TEV-GABARAP-Gly
Plasmid#190930PurposePlasmid for the expression and purification of GABARAP. Internal Reference: SMC894DepositorAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSUMO-LIC-CHIKV-nsP2h
Plasmid#127935PurposeExpresses CHIKV nsP2h (1-465 aa) in bacterial cellsDepositorInsertCHIKV nsP2h
TagsHis6-Sumo-TEVExpressionBacterialPromoterT7 promoterAvailable SinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
6CCR (GID4)
Plasmid#119960PurposeBacterial expression for structure determinationDepositorInsertGID4 (C17orf39 Human)
Tags6xHIS, thrombin,SUMO and TEV cleavageExpressionBacterialMutationcontains amino acids 116-300PromoterT7Available SinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDuex2-iBB-GECO1
Plasmid#194216PurposeFar-red fluorescent Ca2+ ion indicator for mammalian cell and bacterial expression.DepositorInsertiBB-GECO1
Tags6xHisExpressionBacterial and MammalianPromoterCMVAvailable SinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_H3
Plasmid#171927PurposeBacterial expression of nanobody VHH_H3, which binds to receptor binding domain of the spike protein of SARS-CoV-2DepositorInsertVHH_H3
TagsHis6ExpressionBacterial and MammalianAvailable SinceSept. 23, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pST39-Hrs-STAM1 core
Plasmid#21497DepositorTagsHisExpressionBacterialMutationnoneAvailable SinceFeb. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET28aAviHisCD72cCTLD
Plasmid#129066Purposegeneration of biotinylated mouse CD72c CTLD protein using bacteriaDepositorInsertCD72c (Cd72 Mouse)
ExpressionBacterialAvailable SinceMay 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-hTRPT1-iso1
Plasmid#184239PurposeFor purification of N-terminally His-tagged TRPT1 from bacteriaDepositorAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRC072
Plasmid#225982PurposeJ23110_buj_sfGFP_buj_mRFPDepositorInsertsfGFP (with buj RBS), mRFP (with buj RBS)
ExpressionBacterialPromoterJ23110Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
ZFYVE16
Plasmid#39099PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKLlR5
Plasmid#149464PurposeBroad-Host range expression vector, RK2 and pUC origin, LacI repressor, PLlacO-1 promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertLacI-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPLlacO-1Available SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-MRPP1
Plasmid#67862Purposebacterial expression of TRMT10C, full coding sequence (mitoch. form) + N-term. His tag (thrombin site)DepositorInsertMRPP1 (TRMT10C Human)
TagsHisExpressionBacterialMutationmitoch. form (contains aa 40-403)PromoterT7Available SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSL4529 (pTnpA)
Plasmid#210869PurposeExpresses GstTnpA from pLac promoterDepositorInsertGstTnpA
ExpressionBacterialAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSL5200 (pDonor_ISGst3)
Plasmid#210875PurposeHarbors a native ISGst3 transposon derived form Geobacillus stearothermophilus DSM 458DepositorInsertISGst3
ExpressionBacterialAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEPSA5Cas9syn2
Plasmid#206963PurposeS. aureus genome editing vector removed these two motifs AGGNNNNNGAT, CCAYNNNNNNTGT, CCAYNNNNNNGTA, GGANNNNNNNNTCG, and GWAGNNNNNNGATDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
5TTW (EED)
Plasmid#101254PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceOct. 30, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET30b_T7Sav
Plasmid#138588PurposeT7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 oriDepositorInsertT7-tagged streptavidin variant with native C-terminus
UseSynthetic BiologyTagsT7-tagExpressionBacterialPromoterPT7Available SinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
MDA5A
Plasmid#42394PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
ETV1_pet28a
Plasmid#131644PurposeBacterial expression of ETS gene ETV1DepositorAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST cdc25c
Plasmid#10969DepositorAvailable SinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT21.RY
Plasmid#201006PurposepLv1-pUC18R6KT-miniTn7-Gm-mCherry-sYFP2, GmR, ApRDepositorInsertsmcherry
sYFP2
ExpressionBacterialPromoterJ23104Available SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT21.YB
Plasmid#201008PurposepLv1-pUC18R6KT-miniTn7-Gm-sYFP2-TagBFP, GmR, ApRDepositorInsertssYFP2
TagBFP
ExpressionBacterialPromoterJ23104Available SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only