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33,906 results

Showing: 3921 - 3940 of 33906 results

  2. pUC-GFP-MCC

    Plasmid
    #133307
    Purpose
    "Burdensome" GFP expressing plasmid carrying microcin-V bacteriocin for plasmid stabilisation
    Depositor
    Chris Barnes
    Publication
    Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1
    Insert
    microcin-V bacteriocin cassette (cvaC E. coli)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    N112D (please see depositors comments)
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    133307-plasmid-map-sequence-id-272413

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  4. pGGF_RT_FAST_Venus

    Plasmid
    #136980
    Purpose
    Goldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescence
    Depositor
    Klaus Theres
    Insert
    seed coat marker, replaced RFP w/ Venus. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator
    Expression
    Bacterial
    Mutation
    BsaI site domestication in pOLE1:OLE1-RFP 688 G &…
    Available since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    136980-plasmid-map-sequence-id-269080

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  5. KE-005

    Plasmid
    #133440
    Purpose
    Human elF4A1 residues 1-406, N-term His tag, thrombin cleavage, in pET21 vector backbone.
    Depositor
    Gerhard Wagner
    Insert
    EIF4A1 (EIF4A1 Human)
    Expression
    Bacterial
    Available since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133440-plasmid-map-sequence-id-267708

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  6. pSLC-255

    Plasmid
    #124292
    Purpose
    Expression of vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.
    Depositor
    Swaine Chen
    Publication
    Eshaghi et al Angew Chem Int Ed Engl. 2015 Nov 16;54(4
    Insert
    vsfGFP-9
    Expression
    Bacterial
    Available since
    Sept. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    124292-plasmid-map-sequence-id-243084

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  7. pET30a-Tn5

    Plasmid
    #127917
    Purpose
    Prokaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.
    Depositor
    Silin Zhong
    Publication
    Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:
    Insert
    Tn5
    Tags
    Mxe intein - Chitin-binding domain
    Expression
    Bacterial
    Mutation
    E54K, L372P
    Promoter
    T7
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    127917-plasmid-map-sequence-id-254855

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  8. MP28444 – pFUSE2ss-aRD114_CHIg-mIgG2A

    Plasmid
    #110555
    Purpose
    pFUSE backbone with an IL2 signal sequence, expressing the variable heavy chain of anti-RD114 binder fused to murine IgG2A constant heavy chain.
    Depositor
    Martin Pule
    Publication
    Mekkaoui et al J Gen Virol. 2019 Nov 8. doi: 10.1099/jg
    Insert
    Anti-RD114 heavy chain
    Tags
    mIgG2A
    Expression
    Bacterial and Mammalian
    Promoter
    EF-1 alpha core promoter
    Available since
    Aug. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    110555-plasmid-map-sequence-id-212943

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  14. SiriusGFP

    Plasmid
    #123201
    Purpose
    We have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.
    Depositor
    Joseph Santos-Sacchi
    Publication
    Zhong et al J Neurosci Methods. 2019 Feb 1;313:68-76
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SiriusGFP
    Use
    Mouse Targeting
    Tags
    N/A
    Expression
    Bacterial and Mammalian
    Mutation
    eGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…
    Promoter
    CMV
    Available since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    123201-plasmid-map-sequence-id-239192

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  16. pET His6 MBP TEV HDAC8_374

    Plasmid
    #122174
    Purpose
    Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag
    Depositor
    David Christianson
    Publication
    Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-22
    Insert
    His6 MBP TEV HDAC8_374 (HDAC8 Human)
    Tags
    hexahistidine tag and tobacco etch virus protease…
    Expression
    Bacterial
    Mutation
    Residues Met1–Pro7 and H375-V377 have been delete…
    Promoter
    T7
    Available since
    March 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    122174-plasmid-map-sequence-id-237469

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  18. pMAL-SH3(2)-5R

    Plasmid
    #112089
    Purpose
    Bacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.
    Depositor
    Michael Rosen
    Publication
    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi
    Insert
    SH3(2)-5R (NCK1 Human)
    Tags
    His6 and MBP
    Expression
    Bacterial
    Mutation
    construct contains only repeats of the second SH3…
    Promoter
    Lac
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112089-plasmid-map-sequence-id-236082

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  20. pMM643

    Plasmid
    #112533
    Purpose
    pZE2-PLhrtO-lux-hrtR-RBS3-chuA - HrtR RBS variant of plasmid pMM627 (Strength 33545.5 AU), ColE1 origin, KanR
    Depositor
    Timothy Lu
    Publication
    Mimee et al Science. 2018 May 25;360(6391):915-918.
    Inserts
    HrtR
    ChuA
    luxCDABE
    Expression
    Bacterial
    Mutation
    A17P
    Promoter
    J23107, PL(HrtO), and ProD
    Available since
    Feb. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    112533-plasmid-map-sequence-id-216001

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Showing: 3921 - 3940 of 33906 results