30,753 results

1. Ecoli cpGFP

   Plasmid

   #215713

   Purpose

   Expresses cpGFP in Ecoli

   Depositor

   Katharine Diehl

   Article

   Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

   Insert

   cpGFP
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   circularly permuted GFP

   Promoter

   T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCold TDP43 Q331K

   Plasmid

   #27454

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Tags

   Factor Xa and His6

   Expression

   Bacterial

   Mutation

   Glutamine 331 mutated to Lysine (Q331K)

   Available Since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pHnCBS1D

   Plasmid

   #52065

   Purpose

   expresses nine genes from the H. neapolitanus carboxysome operon and CSOS1D; IPTG-inducible

   Depositor

   David Savage

   Article

   Bonacci et al Proc Natl Acad Sci U S A. 2012 Jan 10;10

   Insert

   CbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B, CSOS1D
   

   Expression

   Bacterial

   Available Since

   April 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX6P1-hcGAS(157-522)

   Plasmid

   #108676

   Purpose

   For expression in E.coli and affinity purification of N-terminally GST-tagged human cGAS amino acid 157-522

   Depositor

   Andrew Jackson, Martin Reijns

   Article

   Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

   Insert

   cGAS amino acid 157-522 (CGAS Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains amino acid 157-522

   Promoter

   tac

   Available Since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTECH-MmPylRS(IPYE)

   Plasmid

   #104073

   Purpose

   Expression plasmid encoding MmPylRS(IPYE) and pylT

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Plpp MmPylRS(IPYE); PproK pyl
   

   Expression

   Bacterial

   Mutation

   Contains activating mutations V31I, T56P, H62Y, A…

   Promoter

   Plpp and PproK

   Available Since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMT85_PS-mEos3.2_GentaR

   Plasmid

   #173895

   Purpose

   Transposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the Spiralin promoter

   Depositor

   Yonathan Arfi

   Article

   Rideau et al Microbiol Spectr. 2022 Jun 29;10(3):e006

   Insert

   mEos3.2
   

   Expression

   Bacterial

   Promoter

   Spiralin

   Available Since

   Jan. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET32a-HD16Q

   Plasmid

   #11487

   Depositor

   Pamela Bjorkman

   Article

   Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

   Insert

   huntingtin, exon 1, 16 glutamines (HTT Human)
   

   Tags

   His, His (out of frame), and Trx

   Expression

   Bacterial

   Mutation

   huntingtin exon 1 with 16 glutamines, vector m…

   Available Since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pAM5406

   Plasmid

   #132661

   Purpose

   RSF1010-based broad host-range plasmid with additional RK2 bom site and pUC origin

   Depositor

   Susan Golden

   Article

   Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCR-Zeo

   Plasmid

   #158709

   Purpose

   For cloning and constitutive expression of crRNAs in mycobacterium

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

   Insert

   crRNA
   

   Use

   CRISPR and Synthetic Biology; Mycobacteria expres…

   Expression

   Bacterial

   Promoter

   Hsp60

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. DT037A

    Plasmid

    #159398

    Purpose

    Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23101 (strong) promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.J23101-sfGFP
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Bacterial

    Promoter

    J23101 (strong) promoter

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSFDuet-sumo-h-cGASCD(K427E/K428E)

    Plasmid

    #127162

    Purpose

    expression of truncated human cGAS protein

    Depositor

    Thomas Tuschl

    Article

    Lama et al Nat Commun. 2019 May 21;10(1):2261. doi:

    Insert

    MB21D1 (CGAS Human)
    

    Tags

    His-SUMO tag

    Expression

    Bacterial

    Mutation

    None

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJL33

    Plasmid

    #227430

    Purpose

    Kanamycin resistant L5-integrative vector for CRISPR interference in M.tuberculosis with the constitutive expression of mWasabi under the control of the psmyc promotor (psmyc::mWasabi)

    Depositor

    Pierre Santucci

    Article

    Laudouze et al FEBS Lett. 2025 Feb;599(4):488-501. doi:

    Insert

    mWasabi
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJL34

    Plasmid

    #227431

    Purpose

    Kanamycin resistant L5-integrative vector for CRISPR interference in M.tuberculosis with the constitutive expression of tdTomato under the control of the psmyc promotor (psmyc::tdTomato)

    Depositor

    Pierre Santucci

    Article

    Laudouze et al FEBS Lett. 2025 Feb;599(4):488-501. doi:

    Insert

    tdTomato
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-4T-2/Rabaptin-5:R5BD

    Plasmid

    #168808

    Purpose

    Production of recombinant GST-R5BD (Rab5-binding domain) in bacteria for GST pulldown assays to identify binding partners for Rab5 subfamily members

    Depositor

    Guangpu Li

    Article

    Qi et al Methods Mol Biol. 2015;1298:259-70. doi:

    Insert

    Rab5-binding domain (aa739-862) of Rabaptin-5
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAM5411

    Plasmid

    #132663

    Purpose

    RSF1010-based broad host-range plasmid with RK2 bom site and pUC origin expressing YFP and Sp/Sm resistance

    Depositor

    Susan Golden

    Article

    Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

    Inserts

    aadA
    YFP
    

    Expression

    Bacterial

    Promoter

    PaadA and PconII

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZac2.1 gfaABC1D-Ezr-BioID2-HA

    Plasmid

    #176744

    Purpose

    Expresses Ezrin fused with BioID2 at the C-terminus in astrocytes

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    Ezr-BioID2-BioID2-HA
    

    Use

    AAV

    Tags

    HA

    Expression

    Bacterial

    Promoter

    gfaABC1D

    Available Since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFloat.T7_v1

    Plasmid

    #133070

    Purpose

    Entry vector for cloning genes under a T7 promoter for expression in bacteria. Version 1.0.

    Depositor

    Kacper Rogala, David Sabatini

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    3C-His6 and His6-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pScaf-7560.1

    Plasmid

    #111406

    Purpose

    pScaf phagemid with insert for generating DNA origami scaffold 7560 bases in length

    Depositor

    Shawn Douglas

    Article

    Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

    Insert

    pScaf-7560.1
    

    Expression

    Bacterial

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKP864

    Plasmid

    #167658

    Purpose

    Express colicinB-His6

    Depositor

    Kathleen Postle

    Insert

    cba-his6
    

    Expression

    Bacterial

    Mutation

    cba-his6

    Available Since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET15b-pA-Mnase

    Plasmid

    #124883

    Purpose

    To transform BL21-Gold(DE3) and induced by IPTG for His-proteinA-MNase fusion protein.

    Depositor

    Keji Zhao

    Article

    Ku et al Genome Res. 2021 Apr 14. pii: gr.260893.

    Insert

    protein A-MNase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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