32,672 results
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3xFLAG-CUL1-pCMV7.1
Plasmid#155019PurposeN-terminal 3xFLAG-tagged CUL1 in a pCMV7.1 vectorDepositorAvailable SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRMCD70
Plasmid#167557PurposePlasmid pWSK29 encoding E. coli LacZ' for making reporter fusion proteins for topology mappingDepositorTypeEmpty backboneTagsLacZ'ExpressionBacterialAvailable SinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPIPL12-FnCas12a
Plasmid#199563PurposeTetracycline-Inducible Fncas12a gene expression in E. coli and clostridiaDepositorInsertFncas12a
UseCRISPR and Synthetic Biology; Tetracycline induci…ExpressionBacterialPromoterCOtetR-PIPL12Available SinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPfetFn-Target_v2
Plasmid#199564PurposegRNA expression for FnCas12a in E. coli and clostridiaDepositorInsertSacB
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPthl_fUAvailable SinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETMutS
Plasmid#13245DepositorInsertMutS
ExpressionBacterialMutationNoneAvailable SinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET32a-TRXtag
Plasmid#11516DepositorInsertNone - thioredoxin is vector encoded (trxA )
TagsHis, His (out of frame), and TrxExpressionBacterialMutationvector modified; removed N-terminal His tag by di…Available SinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)_rPD-L1nb
Plasmid#226715PurposeHeterologous expression and purification of recombinant PD-L1 nanobody (rPD-L1nb)DepositorInsertRecombinant PD-L1 nanobody
TagsHA tag and Histidine tagExpressionBacterialPromoterT7Available SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+) pCopA RBS30 sfgfp
Plasmid#226371PurposePlasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with RBS30DepositorInsertsfGFP
Tags6x HisTagExpressionBacterialPromotercopA promoter (pCopA) and RBS30 regulationAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIS003
Plasmid#194014PurposeThis plasmid is designed for use in a procedure to synthesize single-stranded DNA containing only the insert.DepositorInsertC16 Ex4 XTR f1
ExpressionBacterialAvailable SinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Trx
Plasmid#36944DepositorAvailable SinceMay 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-hLIG3
Plasmid#81055Purposebacterial expression of human LIG3DepositorAvailable SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCB’ CbbL Y72R
Plasmid#162707PurposeCarboxysome operon with point mutation to CbbL, prk; Substitution of CsoS2-binding tyrosine with arginine prevents rubisco encapsulation creating rubisco-less carboxysomesDepositorInsertpHnCB10 derived carboxysome operon, prk
ExpressionBacterialMutationCbbL Y72R; Substitution of CsoS2-binding tyrosine…PromoterPLtet0-1 promoterAvailable SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFE-Rrub-FII-prk
Plasmid#162695PurposeExpresses R. rubrum Form II rubisco and S. elongatus PrkDepositorInsertsRubrum Form II rubisco
S. elongatus PRK
ExpressionBacterialPromoterPLtet0-1 promoter and under same promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SpdCas9-LBiT_Strep-Tag-II
Plasmid#198751PurposeExpresses S. Pyogenes dCas9 fused to LargeBiT fragment of split NanoLuc luciferaseDepositorInsertSpdCas9 - LargeBiT
TagsStrep-tag IIExpressionBacterialMutationD10A, H840A (nuclease inactivating mutations in &…PromoterT7Available SinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET22b-mEB3-mCherry-6xHis
Plasmid#130985PurposeBacterial expression of mouse EB3-mCherry-6xHis for protein purification.DepositorAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMApra-Para
Plasmid#218362PurposeEmpty backbone with araC-ParaBAD expression systemDepositorTypeEmpty backboneExpressionBacterialAvailable SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
PJEBAN6
Plasmid#203684PurposeExpresses red fluorescent marker DsRed-Express in Listeria monocytogenesDepositorInsertDsRed-Express
ExpressionBacterialAvailable SinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
plRL58
Plasmid#166886PurposeOptimized M. tuberculosis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634)DepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterTet-OnAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
COVID-SARS2 NSP14/10
Plasmid#159613PurposeBacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10DepositorInsertsTagsHis6, TEV cleavage site and noneExpressionBacterialMutationCodon-optimized for E. coli expressionPromoter(Bicistronic) and T7 - LacOAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only
