32,161 results
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Rb-379-928-pGex2TK
Plasmid#21749DepositorInsertRb (RB1 Human)
TagsGSTExpressionBacterialMutationdeletion mutant ORF contains aa 379-928 of wt Rb …Available SinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTYB2-eIF1A
Plasmid#37218DepositorInserteIF1A
ExpressionBacterialPromoterT7Available SinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNP1 eforRed trigger
Plasmid#107357PurposeTrigger sequence for pNP1 eforRed toehold switchDepositorInserttoehold trigger 1
ExpressionBacterialPromoterT7Available SinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBsVchCAST-pyrE
Plasmid#203813PurposeExpression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coliDepositorInsertVchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(pyrE)
UseCRISPRExpressionBacterialAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBbA5c-MevT-MBI (JBEI-3085)
Plasmid#87950Purposethe overproduction of the universal building blocks to make isoprenoids (i.e. IPP and DMAPP).DepositorInsertMevT-MBI
ExpressionBacterialAvailable SinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE162
Plasmid#118510Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertsGFP2
ExpressionBacterialAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a Gx-NL
Plasmid#169105PurposeExpresses protein G fused to NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - NanoLuc
Tagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available SinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pccGFPGpA
Plasmid#73651PurposeFluorescent reporter for transmembrane protein dimerization positive control.DepositorInsertglycophorin A
ExpressionBacterialAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK642
Plasmid#72391PurposeProduces Escherichia coli glutamine synthetase, N-terminal His6 tag and Y397F mutation that prevents adenylation (H6EcGlnA-Y397F)DepositorInsertglutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
TagsHis6ExpressionBacterialMutationMutated tyrosine-397 to phenylalanine to block ad…PromoterT7Available SinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOPS0381
Plasmid#133231PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter PsNifH (pOGG043), sfGFP (pOGG037) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKCyR6
Plasmid#149493PurposeBroad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Spectinomycin Resistance Marker.DepositorInsertCymR(AM)-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPCymRCAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpyC-GFP
Plasmid#220191PurposesgRNA expression vector - pBG42 promoter with GFP in sgRNA siteDepositorInsertpBG42 promoter with GFP in sgRNA site
UseCRISPRExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG42Available SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-LacI-dCas9
Plasmid#220194Purposemini-Tn7 dCas9 expression vector - LacI/pLac promoterDepositorInsertPseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
UseCRISPRExpressionBacterialMutationPseudomonas aeruginosa codon optimizedPromoterLacI/pLacAvailable SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
PET-B-Sniper SpCas9-NLS-6xHis
Plasmid#207377PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-Sniper SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationF539S, M763I, K890N, amino acids 1005-1013 replac…PromoterT7Available SinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
bfloGFPa1
Plasmid#60749PurposeBacterial expression of Amphioxus GFPa1DepositorInsertGFPa1
Tags6xHisExpressionBacterialPromoterT7Available SinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBK-EcTrpRS(H14)-EcTyrRS(VSMA,D165G)
Plasmid#231130PurposeExpresses mutant E. coli TrpRSTyrRS from a glnS promoters for proteome-wide incorporation of tryptophan/tyrosine analogues.DepositorInsertE. coli tyrosyl tRNA synthetase
ExpressionBacterialMutationEcTrpRS: S8A, V144G, V146C; EcTyrRS: Y37V, D165G,…Available SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_Uricase
Plasmid#182400PurposeBacterial expression of uricaseDepositorInserturicase
ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQCascade-array1
Plasmid#140623PurposeExpresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-array1. The crRNA-array1 targets 8 different loci in Escherchia coli.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, crRNA-array1
UseCRISPR; TransposonExpressionBacterialAvailable SinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-human LIC1 C-terminal half
Plasmid#74599Purposeused for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 389-523DepositorInserthuman dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
TagsN-terminal GST and Strep IIExpressionBacterialMutationamino acids 389-523 of LIC1PromoterT7Available SinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUX19
Plasmid#36328DepositorTypeEmpty backboneUseSuicide vector for r. rubrumExpressionBacterialPromoterreduce strength lacZAvailable SinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
C14orf169
Plasmid#39114PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
6920 pPROEXHsp104b
Plasmid#1230DepositorAvailable SinceAug. 15, 2005AvailabilityAcademic Institutions and Nonprofits only -
pUCSh5vio-2300
Plasmid#170987PurposeEncodes sgRNA target 2300 and Gentamicin resistance - violacein pathway transposon.DepositorInsertSh-CAST sgRNA 2300 violacein
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterplacIAvailable SinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pET28a(+)/FbGH30
Plasmid#86463PurposeExpression of the Beta-1,6-Exo-Laminarinase FbGH30 in E.coliDepositorInsertFbGH30
TagsHis-TagExpressionBacterialPromoterT7Available SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbS8c-ddcpf1-rfp
Plasmid#153038PurposeFor CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array targeting the mrfp reporter gene.DepositorInsertAscpf1
ExpressionBacterialMutationE993AAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
MiCy-pBAD
Plasmid#54565PurposeLocalization: Protein Expression Vector , Excitation: 472, Emission: 495DepositorTypeEmpty backboneTags6xHis and MiCyExpressionBacterialAvailable SinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAH-CTX1-rhadCas9
Plasmid#129391PurposeDerived from pAH-CTX1-rha. The Burkholderia cenocepacia codon-optimized dCas9 cloned downstream of PrhaBAD. Hence, dCas9 is controlled by the rhamnose-inducible promoter system.DepositorInsertBurkholderia cenocepacia codon-optimized dCas9
UseCRISPRExpressionBacterialMutationEntire dCas9 codon optimized for the GC-rich B. c…PromoterPrhaBADAvailable SinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1mCNPRS
Plasmid#174718PurposetRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-4-pyridyl)alanine (mCNP), into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only
