30,753 results

1. pRSET his-mCherry

   Plasmid

   #113552

   Purpose

   For bacterial expression and purification of mCherry

   Depositor

   Jeanne Stachowiak

   Article

   DeGroot et al Biophys J. 2018 Mar 27;114(6):1377-1388.

   Insert

   his-mCherry
   

   Expression

   Bacterial

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-MH6-RspWYL1

   Plasmid

   #108306

   Purpose

   Expresses E. coli codon optimized RspWYL1 in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

   Insert

   RspWYL1
   

   Use

   CRISPR

   Tags

   mH6 tag

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. NylCK_pET28a

   Plasmid

   #215422

   Purpose

   Expression construct for NylCK gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylCK
   

   Expression

   Bacterial

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDSG375

   Plasmid

   #115603

   Purpose

   surface-displayed Nb2; medium copy

   Depositor

   Ingmar Riedel-Kruse

   Article

   Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

   Insert

   regulated adhesin construct
   

   Expression

   Bacterial

   Available Since

   Oct. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pY3

   Plasmid

   #50606

   Purpose

   Origin Of Replication: p15a, Selection Markers: Amp, Promoters: PLacUV5

   Depositor

   Jay Keasling

   Article

   Juminaga et al Appl Environ Microbiol. 2012 Jan;78(1):8

   Inserts

   tyrB
   tyrA
   aroC
   aroA
   aroL
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   lacUV5

   Available Since

   Nov. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSEVA221pmCDA1-T7RNAP

   Plasmid

   #167978

   Purpose

   Expresses fusion protein pmCDA1-T7RNAP in E. coli

   Depositor

   Luis Ángel Fernández

   Article

   Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

   Insert

   pmCDA1-T7RNAP (T7p07 Synthetic)
   

   Tags

   T7 tag and T7RNAP

   Expression

   Bacterial

   Promoter

   TetR-Ptet

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBSV2G_PflaB-iRFPBb

   Plasmid

   #118237

   Purpose

   Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven iRFP

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

   Insert

   flagellin promoter driven iRFP
   

   Expression

   Bacterial

   Available Since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS-QUE7mu

   Plasmid

   #129311

   Purpose

   Low concentration ATP measurement

   Depositor

   Hiroyuki Noji

   Article

   Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

   Insert

   QUEEN-7mu
   

   Expression

   Bacterial

   Available Since

   Oct. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQE81XN-mStayGold(J)

   Plasmid

   #225445

   Purpose

   Expresses Hisx6-Tagged mStayGold(J) in bacteria

   Depositor

   Benjamin Glick

   Insert

   mStayGold(J)
   

   Tags

   6X-His

   Expression

   Bacterial

   Available Since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNIC28-Bsa4-TF

    Plasmid

    #61689

    Purpose

    E. coli expression vector based on pNIC28-Bsa4 (addgene #26103) with the TEE and trigger factor sequences inserted

    Depositor

    Brian Smith

    Type

    Empty backbone

    Tags

    HIS and Trigger Factor

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pH3U3-mcs

    Plasmid

    #12609

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

    Insert

    Multiple cloning site in His3 Ura3 reporter plasmid
    

    Expression

    Bacterial

    Available Since

    Sept. 26, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. Nampt-PET28a

    Plasmid

    #25630

    Depositor

    Cynthia Wolberger

    Article

    Wang et al Nat Struct Mol Biol. 2006 Jul . 13(7):66

    Insert

    Nampt (Nampt Mouse)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Aug. 9, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pB1H1-Zif268

    Plasmid

    #12612

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

    Insert

    Zif268 in pB1H1 (Egr1 Mouse)
    

    Expression

    Bacterial

    Available Since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX Socs3-SH2

    Plasmid

    #46506

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Socs3 (SOCS3 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 22-142

    Promoter

    Tac

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMTTH+bromo5

    Plasmid

    #157803

    Purpose

    Expression of synthetic protein composed of 5 repeats of BRD4 bromodomain #1

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    bromo5
    

    Tags

    His6 and MBP

    Expression

    Bacterial

    Promoter

    pTAC

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRMCD70

    Plasmid

    #167557

    Purpose

    Plasmid pWSK29 encoding E. coli LacZ' for making reporter fusion proteins for topology mapping

    Depositor

    Renato Morona

    Article

    Daniels et al Mol Microbiol. 1998 Jun;28(6):1211-22. d

    Type

    Empty backbone

    Tags

    LacZ'

    Expression

    Bacterial

    Available Since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. GST PTBP1 isoform 4

    Plasmid

    #108594

    Purpose

    Recombinant GST tagged protein production

    Depositor

    Chris Smith

    Article

    Coelho et al EMBO J. 2015 Mar 4;34(5):653-68. doi: 10

    Insert

    PTBP1 isoform 4 (PTBP1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    isoform 4 (isoform a)

    Promoter

    tac

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pE-SUMO CRYGS

    Plasmid

    #80753

    Purpose

    Expression of human gamma S-crystallin in E. coli

    Depositor

    Larry David

    Insert

    CRYGS (CRYGS Human)
    

    Tags

    6xHIS and Smt3 SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJJGL001

    Plasmid

    #180605

    Purpose

    Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Insert

    DpbCasX
    

    Use

    CRISPR

    Tags

    10x His and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHH0103 IQGAP1 WW domain

    Plasmid

    #104279

    Purpose

    Bacterial expression of WW domain from IQGAP1

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    IQGAP1 WW domain (IQGAP1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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