31,059 results

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pQLink-GST-4xCTD

Plasmid

#138471

Purpose

Expresses GST-tagged 4xCTD heptapeptide repeat in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

Rpb1 (POLR2A Human)

Tags

GST

Expression

Bacterial

Mutation

4x CTD repeat

Promoter

T5

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pHIS2-TIP47 (Delta 1-190)

Plasmid

#17640

Depositor

James Hurley

Article

Hickenbottom et al Structure. 2004 Jul . 12(7):1199-207.

Insert

Tip47 (Delta 1-190) (Plin3 Mouse)

Tags

His

Expression

Bacterial

Mutation

Delta 1-190

Available Since

April 3, 2008

Availability

Academic Institutions and Nonprofits only
pHEN-CoV2-nanobody-E2

Plasmid

#194590

Purpose

Bacterial expression of E2 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Gerald McInerney

Article

Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

Insert

E2 (S )

Expression

Bacterial

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pDSG419

Plasmid

#115600

Purpose

surface-displayed Ag2; medium copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Available Since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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Hchk2 (kin-)-PUNI10

Plasmid

#41908

Depositor

Stephen Elledge

Article

Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

Insert

CHK2 (CHEK2 Human)

Expression

Bacterial

Mutation

kinase dead (D347A)

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only
pET28a_MBP_RBFOX2

Plasmid

#224292

Purpose

MBP-RBFOX2 protein purification from bacteria

Depositor

Chuan He

Article

Dou et al Nat Cell Biol. 2023 Sep;25(9):1359-1368.

Insert

RNA binding fox-1 homolog 2 (RBFOX2 Human)

Tags

6xHis and MBP

Expression

Bacterial

Promoter

T7 promoter

Available Since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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YPet-pBAD

Plasmid

#54860

Purpose

Localization: Protein Expression Vector , Excitation: 513, Emission: 527

Depositor

Patrick Daugherty, Michael Davidson

Article

Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

Type

Empty backbone

Tags

6xHis and YPet

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pUC57-Curlcake1

Plasmid

#139340

Purpose

Expresses Curlcake1 (CC1) in E.coli

Depositor

Eva Maria Novoa

Article

Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

Insert

Curlcake1 with T7 promoter

Expression

Bacterial

Promoter

T7

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-Curlcake2

Plasmid

#139341

Purpose

Expresses Curlcake2 (CC2) in E.coli

Depositor

Eva Maria Novoa

Article

Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

Insert

Curlcake2 with T7 promoter

Expression

Bacterial

Promoter

T7

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-Curlcake4

Plasmid

#139343

Purpose

Expresses Curlcake4 (CC4) in E.coli

Depositor

Eva Maria Novoa

Article

Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

Insert

Curlcake4 with T7 promoter

Expression

Bacterial

Promoter

T7

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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GST-GRP78-Full Length (pGEX4T1)

Plasmid

#238421

Purpose

Expresses GRP78 Full Length fused to the GST Tag

Depositor

Amy Shiu Lee

Article

Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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73-21 KRAS PANCS-binder posAP

Plasmid

#240119

Purpose

KRAS G12D PANCS-binder positive AP

Depositor

Bryan Dickinson

Article

Styles et al bioRxiv [Preprint]. 2025 Jan 17:2025.01.

Insert

KRas-RNAPC Pos AP fusion

Expression

Bacterial

Promoter

custom

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pRPCN

Plasmid

#193726

Purpose

Expresses Cas9 from RK2 origin with RFP-marked sgRNA insertion site and kanR/sacB selection/counterselection

Depositor

Martin Buck

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Feb. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pTAKN2_J23119-sgRNA

Plasmid

#108552

Purpose

Bacterial sgRNA expression vector

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Insert

2x Streptococcus pyogenes sgRNA

Expression

Bacterial

Promoter

J23119

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pY002 (pFnCpf1_min)

Plasmid

#69975

Purpose

Expresses FnCpf1 and spacers 1-4 of CRISPR array under artificial promoter. Cas genes are removed.

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

FnCpf1 locus without Cas genes nd artificial promoter

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pET-28b-T7-henAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1935)

Plasmid

#114073

Purpose

T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a-HF1(E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag

Tags

NLS(nucleoplasmin) and 6xHis

Expression

Bacterial

Mutation

E174R, S542R, K548R and N282A

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pET30-T7-sfGFP

Plasmid

#180757

Purpose

pET30 vector missing lacO and N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminator

Depositor

Michael Black

Article

Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

Insert

superfolder green fluorescent protein

Tags

strep tag

Expression

Bacterial

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pET28C-SARS-CoV-2-Nucleocapsid

Plasmid

#204402

Purpose

E. coli expression of full-length SARS-CoV-2 nucleocapsid

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

SARS-CoV-2 nucleocapsid (N SARS-CoV-2)

Tags

6x-His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHEN-CoV2-nanobody-F1

Plasmid

#194592

Purpose

Bacterial expression of F1 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Gerald McInerney

Article

Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

Insert

F1 (S )

Expression

Bacterial

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pQCascade-IS1

Plasmid

#140624

Purpose

Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS1. The crRNA-IS1 targets IS1 loci in Escherchia coli.

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS1

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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