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Bacterial Expression

29,754 results

Showing: 4201 - 4220 of 29754 results

  1. EC71174

    Plasmid
    #154067
    Purpose
    Level 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPER
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Inserts
    HS Cre recombinase
    Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
    HYGROMYCIN Resistance Cassette
    Use
    Cre/Lox and Synthetic Biology; Golden gate
    Tags
    Expression
    Bacterial and Plant
    Mutation
    The Arabidopsis thaliana U5 small nuclear ribonuc…
    Promoter
    35S promoter, Hordeum vulgare HSP 17 promoter use…
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    154067-plasmid-map-sequence-id-313091

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  2. pJMH143

    Plasmid
    #186646
    Purpose
    aadA.co-TaadA-PJ23117-eYFP.co between homology arms for pilA
    Depositor
    Adam Arkin
    Article
    Jacob M. et al bioRxiv
    Insert
    aadA.co-TaadA-PJ23117-eYFP.co
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186646-plasmid-map-sequence-id-377048

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  4. pNR220

    Plasmid
    #79541
    Purpose
    Input plasmid: expresses three serine recombinases (TP901, BxbI, and A118) downstream of three inducible promoters.
    Depositor
    Timothy Lu
    Article
    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.
    Inserts
    TP901
    BxbI
    A118
    Use
    Tags
    His tag and LAA degradation tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    79541-plasmid-map-sequence-id-148753

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  5. pYC2085

    Plasmid
    #158721
    Purpose
    CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using hygromycin as a selection marker.
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364
    Insert
    Sth1 sgRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    158721-plasmid-map-sequence-id-320843

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  8. p4305

    Plasmid
    #198150
    Purpose
    bacterial expression of sifA::HaloTag fusion
    Depositor
    Michael Hensel
    Insert
    PsifA sifA::L16::HaloTag::HA (sifA Salmonella enterica serovar Typhimurium)
    Use
    Tags
    HaloTag-HA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198150-plasmid-map-sequence-id-388083

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  10. pQE80L-IL2 C125S

    Plasmid
    #104348
    Purpose
    Expresses human IL-2 (C125S)
    Depositor
    Alexander McLellan
    Insert
    Human IL-2 (C125S) (IL2 Human)
    Use
    Tags
    MRGSHHHHHGS-tag
    Expression
    Bacterial
    Mutation
    C125S mutation (TGT -> TCC)
    Promoter
    T5
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    104348-plasmid-map-sequence-id-201216

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  16. pExp-Sumo

    Plasmid
    #112574
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Sumo fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112574-plasmid-map-sequence-id-217243

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  19. pPP381

    Plasmid
    #158798
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    dCasPhi-2
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    RuvC-inactivating mutation D394A
    Promoter
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158798-plasmid-map-sequence-id-309523

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Showing: 4201 - 4220 of 29754 results