29,765 results

1. pET28a-Ec.coaD (pESC106)

   Plasmid

   #50388

   Purpose

   Heterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.

   Depositor

   Tadhg Begley, Erick Strauss

   Article

   Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

   Insert

   PPAT
   

   Use

   Tags

   6xHis-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28-AviTag-DogTag-MBP

   Plasmid

   #171773

   Purpose

   Expresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   AviTag-DogTag-MBP
   

   Use

   Tags

   His6 and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Peptide tag added to N-terminus of MBP via a GSGE…

   Promoter

   T7

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-Tag4A-MITF-M (wt)

   Plasmid

   #31151

   Depositor

   Yardena Samuels

   Article

   Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

   Insert

   MITF-M (MITF Human)
   

   Use

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Mutation

   WT

   Promoter

   Available since

   July 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. EYFP-Talin1-FERM

   Plasmid

   #110565

   Purpose

   bacterial expresion of EYFP-tagged Talin 1

   Depositor

   Tina Izard

   Insert

   Talin 1 (Tln1 Mouse)
   

   Use

   Tags

   (His)6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pXW117Ptet2-gfp

   Plasmid

   #160818

   Purpose

   aTc-responsive single-layered circuit

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

   Insert

   PJ23117-30tetR-t-Ptet2-30gfp-t
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23117, Ptet2

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRF-BetI

   Plasmid

   #49357

   Depositor

   Christopher Voigt

   Article

   Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

   Insert

   BetI repressor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET24a MDH_9CREN

   Plasmid

   #204243

   Purpose

   Bacterial expression of I. islandicus malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   MDH_9CREN
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   Promoter

   Lac

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJBL7005

   Plasmid

   #136944

   Purpose

   Malachite green aptamer with stability hairpin, T7 promoter

   Depositor

   Julius Lucks

   Article

   Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

   Insert

   Malachite green aptamer
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. +36GFP-LPETG

   Plasmid

   #71754

   Purpose

   +36GFP-LPETG for expression in E.Coli

   Depositor

   David Liu

   Article

   Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

   Insert

   (GGS)9_(pos36GFP)_6XHis_LPETG
   

   Use

   Tags

   6XHis and Sortase (LPETG)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBS hBAF155

    Plasmid

    #17876

    Depositor

    Jerry Crabtree

    Article

    Wang et al Genes Dev. 1996 Sep 1. 10(17):2117-30.

    Insert

    BAF155 (SMARCC1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pET-Sac-Abeta(MC1-40)

    Plasmid

    #127152

    Purpose

    Expresses N-terminal cysteine Abeta(1-40)

    Depositor

    James Nowick

    Insert

    Abeta(MC1-40) (APP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a(+)- PC-MjMAT

    Plasmid

    #179372

    Purpose

    PC-MjMAT is an engineered methionine adenosyltransferase that catalyses the chemo-enzymatic generation of AdoMet analogs carrying photocaging groups.

    Depositor

    Andrea Rentmeister

    Article

    Peters et al Chembiochem. 2021 Oct 4. doi: 10.1002/cb

    Insert

    PC-MjMAT
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    L147A, I351A

    Promoter

    T7

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. hDHFR

    Plasmid

    #184707

    Purpose

    bacterial expression of human DHFR

    Depositor

    Peter Wright

    Article

    Bhabha et al FEBS Lett. 2011 Nov 16;585(22):3528-32.

    Insert

    DHFR (DHFR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEND-184_Cacnes_mCherry

    Plasmid

    #225610

    Purpose

    Replicative vector for red fluorescent reporter protein mCherry strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCherry.

    Depositor

    Marc Güell

    Article

    Guillermo et al Cell Systems, 2025

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    V5-6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLK

    Plasmid

    #64186

    Purpose

    harbours marker genes LEU2 and LYS2

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Aug. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET-HIS-Sangamo

    Plasmid

    #40786

    Purpose

    for e. coli expression, Golden Gate Compatible TALEN incorporated with N Terminal 6xHIS Tag, Codon Optimized FokI

    Depositor

    Gang Bao

    Insert

    Transcription Activator Like Effector Nuclease
    

    Use

    TALEN

    Tags

    N Terminal 6xHIS

    Expression

    Bacterial

    Mutation

    +63 C Terminus, Codon Optimized FokI Endonuclease

    Promoter

    T7

    Available since

    Dec. 12, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pLG021 (AVAST type 5)

    Plasmid

    #157899

    Purpose

    Expresses the AVAST type 5 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    AVAST type 5 (STAND ATPase with N-terminal SIR2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBA1015

    Plasmid

    #189589

    Purpose

    T7gp1.7 deletion HR vector 250bp HR Arms (T7 phage editing)

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Insert

    T7gp1.7 Locus 250bp Homology Arms
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Encodes for a full deletion of T7gp1.7

    Promoter

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AWP-031

    Plasmid

    #213967

    Purpose

    Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for constitutive CRISPR array expression.

    Depositor

    Alexander Westermann

    Article

    Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121

    Insert

    Cas12a (cas12a Segatella bryantii, Synthetic)
    

    Use

    Tags

    3XFLAG

    Expression

    Bacterial

    Mutation

    D875A to inactivate target nuclease activity

    Promoter

    PcfxA, IPTG inducible

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP-Dynamin 2 K44A

    Plasmid

    #22301

    Depositor

    Pietro De Camilli

    Article

    Ochoa et al J Cell Biol. 2000 Jul 24. 150(2):377-89.

    Insert

    Dynamin 2 K44A (Dnm2 Rat)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    K44A

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only