30,756 results

1. pCK351

   Plasmid

   #129696

   Purpose

   For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120034435

   Depositor

   John Heap

   Article

   Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m

   Inserts

   PrhaBAD
   yfp
   rhaS
   Terminator ECK120034435
   

   Expression

   Bacterial

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-PTBP1CCtoSS

   Plasmid

   #89157

   Purpose

   Recombinant expression of PTBP1 C250S,C251S in E.coli

   Depositor

   Frédéric Allain

   Article

   Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

   Insert

   Polypyrimidine Tract Binding Protein 1 mutant Cys250Ser, Cys251Ser (PTBP1 Human)
   

   Tags

   hexa His

   Expression

   Bacterial

   Mutation

   mutations: Cys 250 to Ser, Cys 251 to Ser

   Promoter

   T7

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVoPo-mSc

   Plasmid

   #187842

   Purpose

   Constitutive mScarlet-I-expression vector for Fusobacterium nucleatum

   Depositor

   Jörg Vogel

   Article

   Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

   Insert

   mScarlet-I
   

   Expression

   Bacterial

   Available Since

   Oct. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBHM2617

   Plasmid

   #234496

   Purpose

   NATR-CnU6-empty sgRNA

   Depositor

   Hiten Madhani

   Article

   Nalley et al bioRxiv 2025.02.14.638277

   Insert

   NAT-PCnU6-sgRNA_scaffold
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Available Since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSP1D1-Strep-II

   Plasmid

   #224209

   Purpose

   Membrane scaffold protein (MSP1D1) with Strep-II tag. pMSP1D1, 7xHis tag swapped for a Strep-II tag.

   Depositor

   Anne Robinson

   Article

   Pettersen et al Biophys J. 2025 Jan 21;124(2):440-457. d

   Insert

   MSP1D1-Strep-II
   

   Tags

   Strep-II Tag

   Expression

   Bacterial

   Mutation

   Substitution of 7xHis tag found in pMSP1D1 for a …

   Promoter

   T7

   Available Since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-6P-acatenin

   Plasmid

   #186458

   Purpose

   Expresses human alpha-catenin with an N-terminal GST tag and a PRescission Protease cleavage site

   Depositor

   Tina Izard

   Article

   Rangarajan et al Commun Biol. 2023 Mar 16;6(1):276. doi:

   Insert

   CTNNA1 (CTNNA1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a(+)-SUMO-A'

   Plasmid

   #209115

   Purpose

   Bacterial expression of CC-GEMS ligand SUMO-A'

   Depositor

   Tom de Greef

   Article

   Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

   Insert

   SUMO-A'
   

   Expression

   Bacterial

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETM33_CLIP_CAP_Gly

   Plasmid

   #178470

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   CLIP_CAP_Gly (CLIP1 Synthetic, Human)
   

   Tags

   His-GST

   Expression

   Bacterial

   Promoter

   T7/LacO

   Available Since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET30-RT1306

   Plasmid

   #131521

   Purpose

   Expresses engineered RT-1306 with His-tag

   Depositor

   Bryan Dickinson

   Article

   Zhou et al Nat Methods. 2019 Dec;16(12):1281-1288.

   Insert

   RT-1306
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTD-CTwinStrep

    Plasmid

    #45939

    Purpose

    use to create C-terminal Twin-Strep-tag fusion proteins

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Tags

    Twin-Strep

    Expression

    Bacterial

    Promoter

    Trc (lactose/IPTG inducible)

    Available Since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET His6 Mocr TEV LIC cloning vector (1O)

    Plasmid

    #29658

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis, Mocr, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSC27

    Plasmid

    #117478

    Purpose

    mobilizable, SRB-stable, kanamycin-resistant cloning vector

    Depositor

    Judy Wall

    Article

    Rousset et al Plasmid. 1998;39(2):114-22. doi: 10.1006

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEQ111m

    Plasmid

    #40190

    Depositor

    Paul Modrich

    Article

    Wright et al J Biol Chem. 1989 Jul 15;264(20):11816-2

    Insert

    EcoRI endonuclease & EcoRI methylase
    

    Expression

    Bacterial

    Mutation

    changed Glutamic Acid 111 to Glutamine

    Promoter

    Lambda PL

    Available Since

    Nov. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pRSFDuet-1_VNp-mNeongreen_OmpA-mCherry

    Plasmid

    #182390

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen and ompA-mCherry fusions

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-mNeongreen-His
    OmpA-mCherry
    

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21c(+)-mtLeuRS

    Plasmid

    #31079

    Depositor

    Linda Spremulli

    Article

    Bullard et al Biochim Biophys Acta. 2000 Feb 29. 1490(

    Insert

    Mature form human mitochondrial leucyl-tRNA synthetase (LARS2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Includes coding sequence for the mature form. Nt …

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pVoPo-02

    Plasmid

    #187837

    Purpose

    Backbone for translational reporter system in Fusobacterium nucleatum

    Depositor

    Jörg Vogel

    Article

    Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRAM18dSFA[MCS]

    Plasmid

    #84685

    Purpose

    Can be used for the transformation of rickettsia

    Depositor

    Ulrike Munderloh

    Article

    Burkhardt et al PLoS One. 2011;6(12):e29511. doi: 10.137

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGDP3 apmA

    Plasmid

    #112881

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    apmA (apmA Staphylococcus aureus)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    Pbla

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-5X1 Flag PA1

    Plasmid

    #15560

    Depositor

    Kai Ge

    Article

    Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

    Insert

    PA1 (PAGR1 Human)
    

    Tags

    Flag and GST

    Expression

    Bacterial

    Available Since

    Aug. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pSABAD14-172

    Plasmid

    #61976

    Purpose

    PhoRadAC6 intein

    Depositor

    Hideo Iwai

    Article

    Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

    Insert

    PhoRadAC6 intein
    

    Tags

    GB1-H6

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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