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pTRC-APEX2

Plasmid

#72558

Purpose

Expression of cytosolic APEX2 in bacteria

Depositor

Alice Ting

Publication

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

APEX2

Tags

His6

Expression

Bacterial

Mutation

A134P relative to APEX

Available since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pGRB

Plasmid

#71539

Purpose

for expression of gRNA in E. coli

Depositor

Tao Chen

Publication

Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

Type

Empty backbone

Expression

Bacterial

Promoter

J231196

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMYC-GFP

Plasmid

#42142

Depositor

Olufunmilayo Olopade

Publication

Chen et al BMC Cancer. 2011 Jun 13;11:246. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

c-Myc (MYC Human)

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)

Plasmid

#35152

Depositor

Jay Keasling, Taek Soon Lee

Publication

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA

Use

Synthetic Biology

Expression

Bacterial

Mutation

R364S in Mevalonate Kinase

Promoter

LacUV5, pTrc

Available since

May 16, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET GFP LIC cloning vector (2GFP-T)

Plasmid

#29716

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, SiriusGFP, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pFLAG-XBP1u-FLuc

Plasmid

#31239

Depositor

Jimtong Horng

Publication

Jheng et al Cell Microbiol. 2010 Jun . 12(6):796-813

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FLAG-XBP1u-FLuc (XBP1 Human)

Tags

Flag and Fluc

Expression

Bacterial, Insect, and Mamm…

Available since

Aug. 16, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pET23b-Casp9-His

Plasmid

#11829

Depositor

Guy Salvesen

Publication

Stennicke et al J Biol Chem. 1999 Mar 26. 274(13):8359-6

Insert

Caspase 9 (CASP9 Human)

Tags

6xHis

Expression

Bacterial

Available since

May 12, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles
Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)

Plasmid

#199604

Purpose

Expression of cell permeable spCas9 in E. Coli.

Depositor

Junwei Shi

Publication

Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

Insert

Cas9-T6N

Use

CRISPR

Expression

Bacterial

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEvol-pAzFRS.1.t1

Plasmid

#73547

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAG

Depositor

Farren Isaacs

Publication

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Inserts

pAzFRS.1.t1
pAzFRS.1.t1

Expression

Bacterial

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHO4d-Cas9

Plasmid

#67881

Purpose

Cas9nlsHis6 Bacterial Expression construct

Depositor

Michael Nonet

Publication

Fu et al Nucleic Acids Res. 2014 Dec 16;42(22):13

Insert

Cas9

Use

CRISPR

Tags

His6 and T7 epitope

Expression

Bacterial

Promoter

T7

Available since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pET15-MHL

Plasmid

#26092

Purpose

SGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.

Depositor

Cheryl Arrowsmith

Type

Empty backbone

Tags

6xHis and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

Sept. 6, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

5 Articles

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pRK1035

Plasmid

#8832

Depositor

David Waugh

Publication

Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

Insert

TVMV protease

Tags

His and MBP (cleaved)

Expression

Bacterial

Mutation

synthetic gene with codons optimized for E. coli

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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GST-RH▲-MNase

Plasmid

#136292

Purpose

Expression of catalytic inactive RNase H fused to micrococcal nuclease

Depositor

Kavitha Sarma

Publication

Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

Insert

RNase HI-D10R-E48R and MNase

Expression

Bacterial

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pUC18-mini-Tn7T-Gm-lux

Plasmid

#64963

Purpose

mini-Tn7 luxCDABE transcriptional fusion vector

Depositor

Herbert Schweizer

Publication

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

lux operon

Expression

Bacterial

Available since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pUC18T-mini-Tn7T-Gm-dsRedExpress

Plasmid

#65032

Purpose

GmR mini-Tn7 vector, mobilizable, for RFP tagging bacteria

Depositor

Herbert Schweizer

Publication

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

dsRedExpress

Expression

Bacterial

Available since

Aug. 14, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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pSUMO1

Plasmid

#52258

Purpose

Bacterial expression of human 6His-tagged SUMO1, SUMO-E1 and SUMO-E2

Depositor

Primo Schaer

Publication

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Inserts

SUMO-1 (SUMO1 Human)
UBE2l (UBE2I Human)
SAE-2 (UBA2 )
SAE-1 (SAE1 Human)

Tags

6His-tag

Expression

Bacterial

Mutation

C-terminal gly-gly

Promoter

T7 and rbs

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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Pet28a_Human_H3.1

Plasmid

#42631

Depositor

Joe Landry

Insert

Human H3.1 (H3C1 Human)

Tags

Untagged

Expression

Bacterial

Promoter

T7

Available since

Feb. 25, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pCMV/SB10

Plasmid

#24551

Depositor

Perry Hackett

Publication

Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

Insert

Sleeping Beauty

Expression

Bacterial

Available since

Oct. 25, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pGEX-4T1 SHP2 WT

Plasmid

#8322

Depositor

Ben Neel

Publication

O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

Insert

SHP2 (PTPN11 Human)

Tags

GST

Expression

Bacterial

Available since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pET28c-F30-Broccoli

Plasmid

#66788

Purpose

Expresses Broccoli in the F30 scaffold in bacterial cells

Depositor

Samie Jaffrey

Publication

Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

Insert

Broccoli in the F30 scaffold

Expression

Bacterial

Promoter

T7

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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