32,370 results
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Plasmid#198468PurposeE. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.DepositorInsert10His-pG-Tn5 (E54K, L372P)
Tags10His-pGExpressionBacterialMutationE54K, L372PPromoterConsensus φ10 T7 PromoterAvailable SinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET22b-6h-GST-TEVp
Plasmid#172887PurposeCodon optimized (E. coli) TEVp fused with GST and his-tagged for purificationDepositorInsertTEV protease
TagsGST solubility tag and HHHHHHExpressionBacterialPromoterT7 promoterAvailable SinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pG108 - K
Plasmid#178039PurposeBacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markersDepositorInsertKanamycin resistance
ExpressionBacterialMutationErmAM has been replaced with Kanamycin for select…Available SinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTurquoise2-pBAD
Plasmid#54844PurposeLocalization: Protein Expression Vector , Excitation: 434, Emission: 474DepositorTypeEmpty backboneTags6x His, T7 tag, and TEV siteExpressionBacterialPromoteraraBADAvailable SinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHO4d-Cas9
Plasmid#67881PurposeCas9nlsHis6 Bacterial Expression constructDepositorInsertCas9
UseCRISPRTagsHis6 and T7 epitopeExpressionBacterialPromoterT7Available SinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLumi-LgBiT
Plasmid#188319PurposeExpression of LgBiT via sigma 70 promoter in E. coliDepositorInsertLgBiT
UseLuciferase and Synthetic BiologyExpressionBacterialPromotersigma 70 promoterAvailable SinceSept. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
LM627
Plasmid#191551PurposeLM627_MSG-AGGA-promoter-ccdb-TTCC-SNAC-hisDepositorInsertccdb
TagsSNAC-tag, HIS-tagExpressionBacterialAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
SepRS(2)/pSertRNA(B4)/EF-Sep
Plasmid#173897PurposeThe plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli.DepositorInsertsSepRS(2)
pSertRNA
EF-Sep
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPomyces-2
Plasmid#61737PurposeStreptomyces expression of codon-optimized Cas9 and custom gRNADepositorInsertssSpCas9
gRNA cassette
UseCRISPRExpressionBacterialMutationBbsI-flanked lacZ cassette inserted in place of s…Promotergapdhp(EL) and rpsL(XC)-BbsIAvailable SinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
HLTV-hTRIM21
Plasmid#104973PurposePurification of His-Lipoyl-hTRIM21 protein from bacteriaDepositorInsertTripartite Motif containing 21 (TRIM21 Human)
TagsHis-LipoylExpressionBacterialPromoterT7Available SinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-RT003-GPM
Plasmid#163383PurposeTo express sGFP module in the G-baToN systemDepositorInsertGFP-PDGFRtm-2A-mCherry (GPM)
UseLentiviralTagsmCherryExpressionBacterial and MammalianPromoterEF1aAvailable SinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NbALFA
Plasmid#159987PurposeFor the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmCherry-NbALFA
Tags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mCherry and NbALFAAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
His6-TEV-CRBN-midi
Plasmid#215330PurposeCRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader designDepositorInsertCRBN (CRBN Human)
Tags6xHis and TEVExpressionBacterialMutationcontains amino acids 41-187 followed by a Gly-Ser…PromoterlacIAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-Sfp
Plasmid#159617PurposeExpression plasmid for Sfp phosphopantetheinyl transferaseDepositorInsertSfp
Tags6HISExpressionBacterialAvailable SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA.3.1.ADRB2-NP
Plasmid#134376PurposeNanoluc complementation assay. Expression of adrenoceptor beta 2 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of signal sequence and Flag epitope at N terminus of ADRB2.DepositorInsertADRB2-NP (ADRB2 Human)
TagsFlag, natural peptide of nanoluciferase, and sign…ExpressionBacterial and MammalianPromoterT7Available SinceAug. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-RhoA
Plasmid#12202DepositorAvailable SinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pPSU2
Plasmid#89566PurposeHigh copy number plasmid 2 to prepare Penn State DNA molecular weight laddersDepositorInserts750 bp EcoRV fragment
3000 bp EcoRV fragment
4000 bp EcoRV fragment
50 bp PstI fragment
100 bp PstI fragment
200 bp PstI fragment
300 bp PstI fragment
400 bp PstI fragment
500 bp PstI fragment
600 bp PstI fragment
1500 bp Psti fragment
4100 bp PstI fragment
ExpressionBacterialAvailable SinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNC02
Plasmid#233644PurposeFor bacterial expression of sfGFP c-terminally tagged with VitelloTagB.DepositorInsertsfGFP with c-terminal VitelloTagB
Tags6-HisExpressionBacterialAvailable SinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJR98
Plasmid#187239PurposeCR3 constant region - hU6 sgRNA promoter flanked by BsmBI sitesDepositorInsertCR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites
ExpressionBacterialAvailable SinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEM526
Plasmid#248740PurposeTranscriptional reporter backbone/entry vector with mGL and constitutive mScarlet-I3 in pNBU2 in pNBU2 integrative Bacteroides plasmidDepositorInsertPBfP1E6-RBS1PLP-mScarletI3-rrnBT2-[GG insertion site]
ExpressionBacterialAvailable SinceJan. 9, 2026AvailabilityAcademic Institutions and Nonprofits only