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Showing: 10951 - 11000 of 29646 results

  3. L57R

    Plasmid
    #34557
    Depositor
    Kevin Truong
    Insert
    L57 (CASP7 Human, A.Sativa (LOV2))
    Use
    Tags
    mRFP
    Expression
    Bacterial and Mammalian
    Mutation
    catalytic domain of caspase-7 was truncated at am…
    Promoter
    CMV, T7
    Available since
    April 24, 2012
    Availability
    Academic Institutions and Nonprofits only

  4. pdCas9-M-4A6

    Plasmid
    #73434
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4A6.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 4A6 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73434-plasmid-map-sequence-id-135334

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  7. pRL542

    Plasmid
    #70282
    Purpose
    ColK-based vector; provides a nicking function to permit conjugal transfer and bears a polylinker for insertion of methylase-encoding genes
    Depositor
    Peter Wolk
    Article
    Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70282-plasmid-map-sequence-id-125718

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  9. pMT-MCS-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80058
    Purpose
    MCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80058-plasmid-map-sequence-id-152592

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  10. pCoofy41

    Plasmid
    #55184
    Purpose
    Insect expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC.
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Use
    Tags
    His10-Stop-OneStrep-Stop-S-tag-Stop-CBP-HPC4-Stop…
    Expression
    Bacterial
    Mutation
    Promoter
    pPH (Polyhedrin promoter)
    Available since
    July 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    55184-plasmid-map-sequence-id-464534

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  12. pBBR1-Rha-ETh_tji49-kan

    Plasmid
    #166669
    Purpose
    Genome engineering of Burkholderia
    Depositor
    Jun Fu
    Insert
    ETh_tji4
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166669-plasmid-map-sequence-id-329553

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  16. pRS_SN_400

    Plasmid
    #170472
    Purpose
    To amplify transcription template containing LacI operators
    Depositor
    Laura Finzi
    Insert
    T7A1, Os, O-, Lambda t1 in lacZ fragment
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7A1
    Available since
    Sept. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    170472-plasmid-map-sequence-id-336808

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  19. pExp-DsbA(SS)

    Plasmid
    #112563
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-DsbA(SS) and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112563-plasmid-map-sequence-id-217221

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  20. SPIN4

    Plasmid
    #73239
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SPIN4 (SPIN4 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73239-plasmid-map-sequence-id-136205

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  25. pDIV489

    Plasmid
    #177697
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.
    Depositor
    Uffe Mortensen
    Article
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    RNR2p (Debaryomyces hansenii ) and TDH3p (Candida…
    Available since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    177697-plasmid-map-sequence-id-352587

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  26. pECT5

    Plasmid
    #75451
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt5 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt5
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    none
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75451-plasmid-map-sequence-id-146479

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  29. pDB026a

    Plasmid
    #109260
    Purpose
    Accessory plasmid for PACE of MjTyrRS variants
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp_pIII(P29*); luxAB; PproK_TyrT(Opt, CUA);
    Use
    Tags
    Expression
    Bacterial
    Mutation
    amber codon at position 29 gIII
    Promoter
    Ppsp; PproK
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    109260-plasmid-map-sequence-id-210477

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  35. pFGL822

    Plasmid
    #58225
    Purpose
    For fungal transformation via ATMT. Transformants to be selected by bialaphos resistance.
    Depositor
    Naweed Naqvi
    Article
    Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,
    Insert
    phosphinothricin acetyl transferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans trpC promoter
    Available since
    June 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    58225-plasmid-map-sequence-id-115839

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  36. pSC201

    Plasmid
    #129387
    Purpose
    A variant of the pSCrhaB2 vector with the oriR6K and mob genes from pGPΩ-TP. Used for homologous recombination of the rhamnose-inducible system into the chromosome of Burkholderia cenocepacia K56-2.
    Depositor
    Silvia Cardona
    Article
    Ortega et al J Bacteriol. 2007 May;189(9):3639-44. do
    Insert
    oriR6K and oriT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N/A
    Promoter
    N/A
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    129387-plasmid-map-sequence-id-257366

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  40. NUDT7 (NUDT7A-c002)

    Plasmid
    #98235
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    NUDT7A (NUDT7 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98235-plasmid-map-sequence-id-193415

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  47. ZP36 Pax2

    Plasmid
    #16906
    Depositor
    Randall Moon
    Insert
    pax2 (pax2a Zebrafish)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2008
    Availability
    Academic Institutions and Nonprofits only

  50. pAL-pgi_T

    Plasmid
    #74066
    Purpose
    pAL374 plasmid carrying the pgi (T) sgRNA, targeting the template strand of pgi, SpecR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    sgRNA pgi (T)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    ptac
    Available since
    April 12, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 10951 - 11000 of 29646 results